Proposal for dbt builder programme


Group 4. Prof.K.Thyagaraju, Department of Biochemistry, SVUniversity, Tirupati



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Group 4. Prof.K.Thyagaraju, Department of Biochemistry, SVUniversity, Tirupati

a. Rationale:

The department of biochemistry, at present, is working on isolation, purification and analysis of various plant various parts products of Tirumala hills on the regulation of microbes, oxidant stress, cell induction, diabetes and obesity of animals. This department is conducting work on production of wine and fermentation to produce bye products as secondary metabolites for the human use. Further the department has established marker proteins for toxicity effect determination in animal systems and use of various plant products as antioxidants to these affected systems. In addition to these further conducting of experiments on cell biology, growth regulation of spermatozoa (rats), embryos (chick), and DNA and protein analysis of cells. Considering the significance work performed of Biochemistry department the following objectives are selected in this programme.


b. Objectives:

  1. To isolate and analyze phytochemicals of selected plants from the hill parts of Tirumala

  2. To study the regulatory effect of phyto chemicals on obesity, diabetes, oxidative stress, toxicity in animals, rats and microbial contamination.

  3. To isolate the secondary products of plant leaves, roots, bark, flower, seed, fruit and tubers, if any and prepare the bulk products for their use in specific activity analysis.

  4. To screen each isolated compound for their spectral, molecular and structural analysis

  5. To prepare phytomedicines for establishment of their pharmacological uses on cells, organs and macromolecules.


c. Deliverables:

The experimental output of the above objectives generates various pure phytomedicines which are used in curing the diseases of skin problems, liver, kidney, heart, pancreas, eye, brain and blood metabolic problems. They also serve as antibiotics to various microbes. The phytochemicals work at the level of induction of immune system, cell regeneration, and removal of abnormal parts like fat, dead cells etc. These phytomedicines can also generate finance to the producer as pharmaceuticals depending on the function for which they are used and made into the forms as tablets, caplets, capsules, syrups and powders.



20. Existing level of collaborations, if any with

A.

Industry

Matrix Biosciences, Hyderabad

CPAquaculture Seed Private Ltd.,Chennai

Nector Vaptech Aquaculture Pvt.Ltd., Eluru

IBGroup of Companies, Chennai

S&S Litopaeneus vernami Hatchery, Mugalthuru,EG Dist

Surya Pharmaceuticals Pvt. Ltd., Varanasi.

Matrix Sea Food India P Ltd, Hyderabad

SRIVT, Hyderabad

SARRA, Bangalore


B.

Medical Colleges/Hospitals:

Sri Venkateswara Medial College (SVMC), Tirupati

Sri Venkateswara Institute of Medical Sciences (SVIMS), Tirupati

Nichi Stem Cell Research Centre, Chennai

KIMS Hospital, Hyderabad

SVRR Government General Hospital, Tirupati

Sri Venkateswara Maternity Hospital, Tirupati

Guntur Medical College (GMC), Guntur

Narayana Medical College (NMC), Nellore

NIMS, Hyderabad

Krishna Teja Dental College, Tirupati



C.

Agriculture colleges/Universities:

Sri Venkateswara Agricultural College, Tirupati

Regional Agricultural Research Station (RARS)

Frontier Technology Laboratory (FTL), Tirupati

Acharya N.G.Ranga Agricultural University, Hyderabad

ICRISAT, Hyderabad,

Indian Institute of Horticulture Research, Bangalore



D

Veterinary colleges/Universities:

University of Delhi, South Campus, Delhi

University of Hyderabad, Hyderabad

S.V.Veterinary College, Tirupati

Y.S.R.Veterinary Univeristy, Tirupati

IVRI, Bangalore, Madras Veterinary University, Chennai


E.

Others:

Indian Immunologicals Pvt. Ltd, Hyderabad

Indian Institute of Science, Bangalore



21. Detailed Work Schedule, Time line & Targets (Year-wise):


Year

Group-1

Group-2

Group-3

Group-4

Animal Cell/Tissue culture and Regenerative biology

Viral Diagnostics

Behavior biology and Biodiversity

Bioactive Molecules /Compounds

2014-2015

Molecular Characterization of bio molecules under In vitro conditions, In silico modeling of their functional capacity.

Isolation, identification of viruses by using immunological and molecular methods

Floristic and Taxonomic Studies, Ethno medicinal Studies, Chemo Taxonomical, Growth

Screening of plants, Isolation and identification of plant products.

2015-2016

In vitro testing of anti-microbial and anti-inflammatory and anti-biotic efficacy of the bio molecules. Molecular and gene expression studies.

Cultivation and Molecular

Characterization of viruses



Developmental Studies, Secondary Metabolite Production and Medicinal Applications, Pharmacognostic Studies, Analysis of Phytoconstituents, Biological Evaluation of Herbal Drugs

Molecular characterization and establishement of medicinal properties of Bioactive compounds.

2016-2017

Development of Stem cells from animal models and Invivo / In vitro induction of various abnormal health conditions and their regulation by using Stem cells

Cloning and expression of viral proteins/ their products (non-structural/structural)

In heterologus systems



In-vitro and in- vivo Conservation of Rare and Endangered Slant Species (Micro Propagation and Tissue Culture), development of

Drought tolerant agricultural crops using gene manipulation and micropropagation.



Synthesis of Analgoues and production of compounds.

2017-2018

Evaluation of regenerative capacity of stem cells under in vivo modeling

Development of protein and nucleic acid based diagnostics.

Data Documentation of NTFP (Non Timber Forest Products)

Clinical trains of the active biomolecules and their analogues on various organisms.

2018-2019

Industrial

Production and procurement of lead molecules for commercialization.



Field evaluation of viral diagnostics to study their efficacy, patenting of viral diagnostics and finally transfer of developed technology to industrial linkage through the DBT.

Digitalization of Plant Resources, Biodiversity Conservation

Prepration of Bioactive compounds in the form of syrups, extracts, tablets, poweders etc.,


22. Have you read the purpose of DBT scheme advertised in Newspapers/posted on the DBT WEBSITE? Through Web Site

If yes, what transformation in future research, education and innovation you expect that the DBT support proposed in life sciences by your university can bring about or what is your vision in applying for the grant under this programme.



Future research plan: Establishement of stem cell research centre, Development of clinical viral diagnostic laboratories, Development of digital data documentation and consevaration of rare and endangered species of plants and animals in Seshachalam forests, Development of drug research and docking laboratory.

Education and Innovation expectation: Based on the outcome of the research results it is expected to propose an education centre on stem cells and their applications, development of drought tolerant plants and agricultural crops for the drought area of Rayalaseems region, to develop markers for various diseases and tools for rapid and low cost diagnostics of viral diseases and to produce various pharmacological products for the diagnized diseases of this region and their efficacy.

Vision of the University: To strengthen the life sceicne courses of our university by modifying them into an advanced research centres with advanced equipment facilities to make an state of art laboratories to suit the applications of biotelchnology.

23. What are the expected limitations/risks in implementation in this programme in case of sanction of DBT proposal submitted by you?

  • The University has capacity to implement and oversee the risks that may appear during the course of the programme.

  • The implementation of the programme is doen according the norms of the university.

Future limitations:

  • Faculty and technical personale recruited for this programme, BUILDER, may not be agreed to absorb to university by government.

  • To sustain the facility it is necessary to finance the recruited personale and maintenance by providing recurring grant from the end of the BUILDER programme.



* Deduct the details of contribution from university and private industry.

24. Budget Particulars: BUDGET IN LAKHS Rs.

Items

1st Year

2nd Year

3rd Year

4th Year

5th Year

TOTAL

A. Non-recurring
1. State-of-the art Labs for Teaching
2. Common facilities 3.Equipment for Research (separate list may be enclosed)

600.00

1200.00


500.00

-

-

-

-

-

Sub-Total (A)

2300.00

-

-

-

-

2300.00

B. Recurring



















Manpower (faculty salaries – Assistant Professor-4, Fellowships- JRF-4+HRA, SRF-4+HRA, Research Associate-4+HRA, Technical Assistant-12 )

57.98

61.36

68.06

73.50

79.46

340.36

Consumables

50.00

50.00

50.00

50.00

50.00

250.00

Laboratory Training Workshops

10.00

10.00

10.00

10.00

10.00

50.00

Contingencies (including maintenance of equipment)

5.00

5.00

5.00

5.00

5.00

25.00

Books & Periodicals

5.00

5.00

5.00

5.00

5.00

25.00

Sub-Total (B)

127.98

131.36

138.06

143.50

149.46

690.36

*Total (A+B) in Crores Rs.

2427.98

131.36

138.06

143.50

149.46

2990.36

Total support sought from DBT: Rs. 2990.36lakhs

Contribution from SVUniversity: Rs. 513.00lakhs
The following Infrastructure will be provide to run the BUILDER Programme.


  1. Multi Seminar Complex built up area (28,000 sft) under construction

Approximate cost: Rs. 350.00 lakhs

  1. Animal House: RCC framed structure ground floor (2374 sft)

and also first floor (1155 sft) Approximate cost of the building: Rs. 53.00 lakhs

  1. Snail Rearing Centre: RCC framed structure (1070 sft) with

AC sheets (4815 sft) Approximate cost: Rs. 35.00 lakhs

  1. Extension of Natural Science Building western wing Approved cost Rs. 50.00 lakhs

  2. Glass house (1800 sft) Approximate Cost: Rs. 25.00 lakhs


Detailed Justification of Budget item-wise:

1. State-of-the art Labs for Teaching


2. Common facilities

3. Equipment for Research (separate list may be enclosed)


1. State of the art labs for teaching and training: Stem Cell Biology Course and laboratory facility: SV is proposing to start M.Sc., Stem Cell Biology an interdisciplinary course which is essential to train the students for providing hands-on-skill experience to cater the needs of hospitals available in the drought prone area of Rayalaseema region which is useful to treat the patients suffering with various diseases/disorders. The Innovate course on Stem Cell Biology is essential for production of stem cells for animal models and study of regenerative capacity under in vivo conditions. The faculty and the man power proposed in the BUILDER proposal are necessary for both teaching and research on Stem Cells. The SVUniversity is also made an MoU with Nichi-Stem Cell Research Centre of Chennai, a branch of Japan which will help us to strengthen the course. This will be helpful to the university to strengthen the advanced research with multidisciplinary approach. The grant also be essential for modernization and establishment of digital and on line e class rooms, WiFi facility, e-governance of life science labs, which can be used to impart advanced training for young faculty, post-doctoral, doctoral and post graduate life sciences students of this campus and other institutes of this region.
Requirements: Rs.600 lakshs


Clean room facility.

100.0

Biosafety level II cabinet.

100.0

Flow cytometry

100.0

Culture Mintanence Facility

100.0

Real time PCR-

30.0

CO2 incubator (air jacket)-

15.0

Table top cooling centrifuge-

15.0

Inverted Microscopes with fluorescence -

10.0

-800C vertical deep freezer

10.0

Temperature controlled Liquid Nitrogen container-

5.0

Other Minor Equipments

115.0


2. Common facilities to be required: The number of labs to be required under Builder programme: Rs.1200 lakhs

1. Cold room facility: Screening, isolation, identification of biomolecules. Rs. 50 lakhs

Requirements: Cold room with illumination, humidity detectors, timers, working benches.

2. Animal cell culture laboratory: In vitro testing of the antimicrobial, anti-inflammatory and antibiotic efficacy of the biomolecules by using various animal cell lines and cultures.

Requirements: CO2 incubator (air jacket), Inverted microscope, Biosafety level III cabinet, -800C vertical deep freezer, Temperature controlled Liquid Nitrogen container, FACS, Fluorescent microscope, Automated blood analyzer, Automated biochemical analyzer, Automated cell analyzer. Rs. 100 lakhs

3. Molecular biology laboratory: Isolation, characterization of genomes, proteins/enzymes and substrates. Rs. 100 lakhs

Requirements: Gradient PCR machine, QPCR, PCR workstation, Microarray, In Situ hybridization high through out analyzer, High speed refrigerated table top centrifuge with variable rotors, Electrophoresis units, 2D electrophoresis unit and Blotting units- Western, Sothern, Hybridization oven, DNA cross linker, Speedvac, -800C vertical deep freezer, , -200C vertical deep freezer, Clean bench facility, Liquid handling facility, Gel doc system, Ultra-pure water system, HPLC/FPLC, Incubator shaker, Gel rockers, Sterilization facility- dry, wet and filter systems, Ultrasonicator.

4. Biotechnology lab: Gene expression studies of various bioactive molecules by using different cloning strategies. Rs. 150 lakhs

Requirements: Gene gun/projectile, Vertical Laminar airflow unit, short nucleotide DNA sequencer, 37oCIncubator, Incubator shaker, Gradient PCR machine, Refrigerated Microfuge, Electrophoresis and Blotting units- Western, Sothern, Hybridization oven, DNA cross linker, Speedvac, -800C and -200C vertical deep freezers, Ultrasonicator,

5. Plant and tissue culture lab: Cultivation of various endangered species and in vitro synthesis of secondary metabolites, testing of plants for transgenic.

Requirements: Tissue culture cabinet/chambers, Horizontal laminar flow bench, Dissection microscopes, Vertical microscope with high magnification, Microtomes, Incubators, Sterilization facility- dry, wet and filter systems. Rs. 50 lakhs

6. Virology laboratory: This laboratory is essential for isolation, cultivations, characterization and maintenance of various viruses and also to develop the immune and molecular based diagnostics.

Requirements: Transmission Electron Microscope, DNA sequencer, Ultracentrifuge floor model, High speed refrigerated centrifuge with variable rotors, ELISA readers, Gel doc system, 37o C Incubator, Incubator shaker, Gradient PCR machine, Refrigerated Microfuge, Electrophoresis and Blotting units- Western, Sothern, Hybridization oven, DNA cross linker, Speedvac, -800C and -200C vertical deep freezers. Rs. 100 lakhs

7. Drug discovery and Pharmacology lab: The Drug discovery is useful to isolate molecules from various organisms and their analogues will be synthesized and prepared in the form of various medicinal constitutents. After the synthesis of all these molecules they will be tested using organzims like microbes, animals and humans in Pharmacology laboratory using the available tools of this laboratory. Rs. 100 lakhs

Requirements: FTIR, LCMS, UV-Vis Sectrophotomter, HPLC, High speed centrifuge and other minor equipments.

8. Molecular modeling and Bioinformatics lab: In Silico modeling of lead molecules and study their functional capacity by using various bioinformatic tools. Rs. 100 lakhs

Requirements: High speed computer systems- servers and various commercial soft wares and packages, data storage system, networking, online backup, highspeed internet facility.

9. Environmental toxicology laboratory: This lab is essential for analysis of environmental toxic compounds and their role on induction health disorders. Rs. 40 lakhs

Requirements: Atomic absorption spectrophotometer,

10. Modernization of animal house facility: The existing animal house can be upgraded according to the animal ethical committee standards to cater the needs of the people who are working on animal models for various In vivo studies. Rs. 100 lakhs

Requirements: Computer controlled temperature system, animal cages, Modern hygienic racks, Animal rearing systems, Animal slotter equipments.

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