Title:Streptavidin-functionalized capillary immune microreactor for highly efficient chemiluminescent immunoassay
Author affiliation:(1) State Key Laboratory of Analytical Chemistry for Life Science, Department of Chemistry, Nanjing University, Nanjing 210093, China; (2) College of Chemistry and Engineering, Yangzhou University, 88 South University Avenue, Yangzhou 225002, China; (3) Jiangsu Institute of Cancer Prevention and Cure, Nanjing 210009, China
Abbreviated source title:Anal. Chim. Acta
Publisher:Elsevier, P.O. Box 211, Amsterdam, 1000 AE, Netherlands
Abstract:A streptavidin functionalized capillary immune microreactor was designed for highly efficient flow-through chemiluminescent (CL) immunoassay. The functionalized capillary could be used as both a support for highly efficient immobilization of antibody and a flow cell for flow-through immunoassay. The functionalized inner wall and the capture process were characterized using scanning electron microscopy. Compared to conventional packed tube or thin-layer cell immunoreactor, the proposed microreactor showed remarkable properties such as lower cost, simpler fabrication, better practicality and wider dynamic range for fast CL immunoassay with good reproducibility and stability. Using α-fetoprotein as model analyte, the highly efficient CL flow-through immunoassay system showed a linear range of 3 orders of magnitude from 0.5 to 200ngmL-1 and a low detection limit of 0.1ngmL-1. The capillary immune microreactor could make up the shortcoming of conventional CL immunoreactors and provided a promising alternative for highly efficient flow-injection immunoassay. © 2011 Elsevier B.V.
Controlled terms:Cell immobilization - Chemiluminescence - Proteins - Scanning electron microscopy
Uncontrolled terms:Capillary - Fetoprotein - Immunoassay - Micro-reactor - Streptavidin
Classification code:461.8 Biotechnology - 461.9.1 Immunology - 741.1 Light/Optics - 804.1 Organic Compounds
Compilation and indexing terms, Copyright 2011 Elsevier Inc.
Accession number:20111913961702
Title:Comparison of the crystalline properties and structural changes of starches from high-amylose transgenic rice and its wild type during heating
Authors:Wei, Cunxu (1); Qin, Fengling (1); Zhou, Weidong (2); Xu, Bin (2); Chen, Chong (2); Chen, Yifang (2); Wang, Youping (1); Gu, Minghong (1); Liu, Qiaoquan (1)
Author affiliation:(1) Key Laboratories of Crop Genetics and Physiology of the Jiangsu Province, Plant Functional Genomics of the Ministry of Education, Yangzhou University, Yangzhou 225009, China; (2) Testing Center, Yangzhou University, Yangzhou 225009, China; (3) Agricultural College, Yangzhou University, Yangzhou 225009, Jiangsu, China
Corresponding author:Gu, M.(gumh@yzu.edu.cn)
Source title:Food Chemistry
Abbreviated source title:Food Chem.
Volume:128
Issue:3
Issue date:October 1, 2011
Publication year:2011
Pages:645-652
Language:English
ISSN:03088146
CODEN:FOCHDJ
Document type:Journal article (JA)
Publisher:Elsevier Ltd, Langford Lane, Kidlington, Oxford, OX5 1GB, United Kingdom
Abstract:A transgenic rice line (TRS) enriched amylose and resistant starch has been developed by antisense RNA inhibition of starch branching enzymes. In this study, gelatinisation and crystalline properties, swelling power, water solubility, morphological and structural changes of starches from TRS and its wild-type Teqing (TQ) were carefully investigated during heating. Compared to TQ, TRS starch showed higher gelatinisation temperatures, lower gelatinisation enthalpy and swelling power. Morphological and structural changes showed that TQ starch drastically swelled after 70 °C, then gradually disrupted with increasing heating temperature. The surrounding band of TRS starch restrained granule swelling, though the subgranules disrupted to form the cavity. The results of spectroscopic analyses indicated that A-type crystalline of TQ changed to amorphous starch after 75 °C, while C-type crystalline of TRS gradually changed to B-type crystalline after 75 °C, then became amorphous starch at 95 °C. These results add to our understanding of the effect of heating on the high-amylose rice starch. © 2011 Elsevier Ltd. All rights reserved.
Number of references:29
Main heading:Starch
Controlled terms:Crystalline materials - Enzyme inhibition - Gelation - Granulation - Heating - RNA - Spectroscopic analysis - Structural properties
Uncontrolled terms:Amorphous starch - Antisense RNA - Crystalline properties - Gelatinisation - Granule swelling - Heating temperatures - High-amylose starch granule - Morphological structures - Resistant starch - Rice - Rice starch - Starch-branching enzymes - Structural change - Swelling power - Transgenic rice - Water solubilities - Wild types
Classification code:933.1 Crystalline Solids - 804.1 Organic Compounds - 802.3 Chemical Operations - 951 Materials Science - 801 Chemistry - 461.2 Biological Materials and Tissue Engineering - 408 Structural Design - 643.1 Space Heating
DOI:10.1016/j.foodchem.2011.03.080
Database:Compendex
Compilation and indexing terms, Copyright 2011 Elsevier Inc.
Accession number:20113814351489
Title:Banded spherulites of electrospun poly(trimethylene terephthalate)/carbon nanotube composite mats
Authors:Wu, Defeng (1); Yang, Tao (1); Sun, Yurong (1); Shi, Tiejun (2); Zhou, Weidong (3); Zhang, Ming (1)
Author affiliation:(1) School of Chemistry and Chemical Engineering, Yangzhou University, Jiangsu 225002, China; (2) Institute of Chemical Engineering, Heifei University of Technology, Anhui 230009, China; (3) Testing Centre, Yangzhou University, Jiangsu 225002, China
Corresponding author:Zhang, M.(dfwu@yzu.edu.cn)
Source title:Polymer International
Abbreviated source title:Polym. Int.
Volume:60
Issue:10
Issue date:October 2011
Publication year:2011
Pages:1497-1503
Language:English
ISSN:09598103
E-ISSN:10970126
Document type:Journal article (JA)
Publisher:John Wiley and Sons Ltd, Southern Gate, Chichester, West Sussex, PO19 8SQ, United Kingdom
Abstract:The crystallization of poly(trimethylene terephthalate) (PTT) composites containing carbon nanotubes (CNTs) were studied in this work. The electrospinning technology was employed successfully to fabricate thin film samples with well-embedded CNTs for spherulite observations using atom force microscopy. The results show that the composites present a higher overall crystallization rate than that of the neat PTT due to the nucleation effect of the CNTs. Banded spherulites can be observed on both the neat PTT and the composites. The presence of CNTs does not change the twisting mode of PTT crystal, but reduces band spacing and twist period. This is attributed to the enhanced fold staggering level of lamellae caused by the narrowed lamellae size and accelerated spherulite growth, which is further confirmed by analysis through secondary nucleation theory. © 2011 Society of Chemical Industry.
Number of references:36
Main heading:Carbon nanotubes
Controlled terms:Carbon - Composite materials - Crystallization - Electrospinning - Nucleation
Uncontrolled terms:Banded spherulite - Crystallization rates - Electrospuns - Force microscopy - Nanotube composites - Nucleation effect - Poly (trimethylene terephthalate) - Secondary nucleation - Spherulite growths
Classification code:933.1.2 Crystal Growth - 933.1 Crystalline Solids - 819.3 Fiber Chemistry and Processing - 951 Materials Science - 811 Cellulose, Paper and Wood Products - 761 Nanotechnology - 415 Metals, Plastics, Wood and Other Structural Materials - 804 Chemical Products Generally
DOI:10.1002/pi.3108
Database:Compendex
Compilation and indexing terms, Copyright 2011 Elsevier Inc.
Accession number:20112114000563
Title:Application of the SWAP model to simulate the field water cycle under deficit irrigation in Beijing, China
Authors:Ma, Ying (1); Feng, Shaoyuan (2); Huo, Zailin (2); Song, Xianfang (1)
Author affiliation:(1) Key Laboratory of Water Cycle and Related Land Surface Processes, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing, 100101, China; (2) Center for Agricultural Water Research in China, China Agricultural University, Beijing, 100083, China; (3) Yangzhou University, Yangzhou, 225009, China
Corresponding author:Feng, S.(fsy@cau.edu.cn)
Source title:Mathematical and Computer Modelling
Abbreviated source title:Math. Comput. Model.
Volume:54
Issue:3-4
Issue date:August 2011
Publication year:2011
Pages:1044-1052
Language:English
ISSN:08957177
CODEN:MCMOEG
Document type:Journal article (JA)
Publisher:Elsevier Ltd, Langford Lane, Kidlington, Oxford, OX5 1GB, United Kingdom
Abstract:The evaluation of the field water cycle under deficit irrigation plays an important role in studying mechanism of field water dynamics, optimization of agricultural water management strategies, and assessment of regional water resources. In this study, the agro-hydrological Soil-Water-Atmosphere-Plant (SWAP) model was used to evaluate the field water cycle for a winter wheat-summer corn double cropping system in Beijing, China under deficit irrigation. A carefully designed field experiment was carried out from 2007 to 2009 with six irrigation treatments. The SWAP model was calibrated with soil water contents of two treatments. The dataset of the main field water balance components including soil water content, profile water storage and water flux through the bottom of the root zone were used to validate the SWAP model. The average root mean square error (RMSE) and the mean relative error (MRE) values of predicted soil water contents were 2.4% and 8.0%, respectively. The dataset of predicted and measured values were close to the 1:1 scale line for both the profile water storage and soil water flux. As an application of the SWAP model, the optimal irrigation management practices for the hydrologic years of 75%, 50% and 25%, respectively, in the Beijing area were obtained. The simulated average amount of water saving and groundwater recharge under the optimal irrigation schedules were about 190 mm and 16.1 mm, respectively. This study indicates that the SWAP model can be used as a powerful tool to simulate the field water cycle and evaluate irrigation practices. © 2010 Elsevier Ltd.
Number of references:19
Main heading:Water content
Controlled terms:Geologic models - Irrigation - Optimization - Rating - Recharging (underground waters) - Soil moisture - Water conservation - Water management
Uncontrolled terms:Agricultural water management - Beijing , China - Beijing area - Data sets - Deficit irrigation - Double cropping - Field experiment - Field water cycle - Ground water recharge - Irrigation management - Irrigation practices - Irrigation schedule - Irrigation treatments - Mean relative error - Regional water resources - Root mean square errors - Root zone - Soil water content - Soil water fluxes - SWAP model - Water balance components - Water cycle - Water dynamics - Water flux - Water saving - Water storage
Classification code:921.5 Optimization Techniques - 902.2 Codes and Standards - 821.3 Agricultural Methods - 483.1 Soils and Soil Mechanics - 481.1 Geology - 444.2 Groundwater - 444 Water Resources
DOI:10.1016/j.mcm.2010.11.034
Database:Compendex
Compilation and indexing terms, Copyright 2011 Elsevier Inc.
Accession number:20112114000543
Title:Application of the SWAP model to simulate water-salt transport under deficit irrigation with saline water
Authors:Jiang, Jing (1); Feng, Shaoyuan (1); Huo, Zailin (1); Zhao, Zhicai (3); Jia, Bin (1)
Author affiliation:(1) Center for Agricultural Water Research in China, China Agricultural University, Beijing, 100083, China; (2) College of Hydraulic Science and Engineering, Yangzhou University, Yangzhou, 225009, China; (3) China Water Resources Beifang Investigation Design and Research Co. Ltd., Tianjin, 300222, China
Corresponding author:Feng, S.(fsy@cau.edu.cn)
Source title:Mathematical and Computer Modelling
Abbreviated source title:Math. Comput. Model.
Volume:54
Issue:3-4
Issue date:August 2011
Publication year:2011
Pages:902-911
Language:English
ISSN:08957177
CODEN:MCMOEG
Document type:Journal article (JA)
Publisher:Elsevier Ltd, Langford Lane, Kidlington, Oxford, OX5 1GB, United Kingdom
Abstract:The agro-hydrological Soil-Water-Atmosphere-Plant (SWAP) model was calibrated and validated to simulate water-salt transport based on field experiments in an arid region of China. The simulation results show lower soil water content but higher salt concentration under deficit irrigation. Soil water and salinity below 95 cm at 80% evapotranspiration (ETc) treatments and 65 cm at 60% ETc treatments were hardly affected by irrigation. With deficit irrigation, the maximum water uptake and salt accumulated layer moved upward. The SWAP model was also used to predict long-term deficit irrigation with saline water. The salinization process reached equilibrium after utilization of saline water for a few years. In summary, the numerical model proves to be a useful tool for studying water-salt transport under different scenarios and for evaluating irrigation practices for a long period. © 2010.
Number of references:26
Main heading:Saline water
Controlled terms:Arid regions - Geologic models - Irrigation - Soil moisture - Water content - Water supply
Uncontrolled terms:Deficit irrigation - Field experiment - Irrigation practices - Numerical models - Salinization process - Salt concentration - Simulation - Simulation result - Soil water - Soil water content - SWAP model - Water uptake
Classification code:444 Water Resources - 446.1 Water Supply Systems - 481.1 Geology - 483.1 Soils and Soil Mechanics - 821.3 Agricultural Methods
DOI:10.1016/j.mcm.2010.11.014
Database:Compendex
Compilation and indexing terms, Copyright 2011 Elsevier Inc.
Accession number:20110413627472
Title:Research progress on chemical modification of alginate: A review
Authors:Yang, Ji-Sheng (1); Xie, Ying-Jian (1); He, Wen (1)
Author affiliation:(1) School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, China
Corresponding author:Yang, J.-S.(jsyang@yzu.edu.cn)
Source title:Carbohydrate Polymers
Abbreviated source title:Carbohydr Polym
Volume:84
Issue:1
Issue date:February 11, 2011
Publication year:2011
Pages:33-39
Language:English
ISSN:01448617
CODEN:CAPOD8
Document type:Journal article (JA)
Publisher:Elsevier Ltd, Langford Lane, Kidlington, Oxford, OX5 1GB, United Kingdom
Abstract:This review summarizes results of the recent research on chemical modifications of alginate that are as yet relatively unexploited commercially. Alginate is a linear, anionic polysaccharide consisting of two kinds of 1,4-linked hexuronic acid residues, namely β-d-mannuronopyranosyl (M) and α-l-guluronopyranosyl (G) residues, arranged in blocks of repeating M residues (MM blocks), blocks of repeating G residues (GG blocks), and blocks of mixed M and G residues (MG blocks). Alginate has an abundance of free hydroxyl and carboxyl groups distributed along the polymer chain backbone, and it, therefore, unlike neutral polysaccharides has two types of functional groups that can be modified to alter the characteristics in comparison to the parent compounds. Methods used for modification of hydroxyl groups of alginate include oxidation, reductive-amination, sulfation, copolymerization and coupling of cyclodextrin units. Methods used for modification of carboxyl groups include esterification, use of the Ugi reaction, and amidation. Furthermore, the characteristics and applications of some alginate derivatives are also summarized. © 2010 Elsevier Ltd. All rights reserved.