Electronic Posters: Cardiovascular
Tuesday 13:30-15:30 Computer 40
Yüklə 492,08 Kb.
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| Tuesday 13:30-15:30 Computer 40
13:30 3745. 3.0T MRI of Auto-Transplantation of Bone Marrow-Derived Stem-Progenitor Cells: Toward Cell-Based Repair of Injured Arteries Yanfeng Meng1,2, Feng Zhang1, Tiffany Blair1, Huidong Gu1, Hongqing Feng1, Jinnan Wang3, Chun Yuan1, Zhaoqi Zhang2, Bensheng Qiu1, Xiaoming Yang1 1Radiology, University of Washington, Seattle, WA, United States; 2Radiology, Beijing Anzhen Hospital, Beijing, China; 3Clinical Sites Research Program, Philips Research North America, Briarcliff Manor, NY, United States This study was to validate the feasibility of using clinical 3.0T MRI to monitor the migration of auto-transplanted bone marrow cells (BMC) to the injured arteries of near-human-sized animals. BMCs were extracted endogenously, labeled with Feridex and/or PKH26, and then auto-transplanted back to the same animal. Post-cell transplantation 3.0T T2-MRI showed Feridex-created MR signal voids along the injured iliofemoral artery segments, which were not seen in the control arteries. Histology, including Prussian blue and dextran immunofluorescent staining as well as PKH26 fluorescence, confirmed the MRI findings. This study establishes groundwork for clinical 3.0T MRI of cell-based repair of injured arteries. 14:00 3746. A Multi-Echo Technique for Positive Contrast Detection of SPIO-Labeled Cells at 9.4T Philip Lee1, Johannes Riegler2, Bingwen Zheng1, Anthony Price2, Mark F. Lythgoe2, Xavier Golay3 1Singapore Bioimaging Consortium, Biomedical Sciences Institute, Singapore, Singapore; 2Centre for Advanced Biomedical Imaging, University College London, London, United Kingdom; 3Institute of Neurology, University College London, London, United Kingdom Migration of super-paramagnetic labeled cells critically affects the success of therapeutic cell studies. Detection with T2* weighted MRI is normally implemented. But direct association of signal voids with SPIOs-labeled cells is erroneous, as they could originate from magnetic field inhomogeneities or partial volume effects. This study highlights the use of a multiple-echo ultra-short echo time (MUTE) sequence for positive contrast visualization of injected mononuclear cells. 5x105 and 2.5x105 of MNCs were directly injected into the left myocardium wall at the apex and mid-ventricle respectively and the heart was subsequently excised for MRI. Subtraction between the UTE (TE=0.208ms) and ECHO (TE=2.56ms) images exploited the transverse relaxation effect of iron, generating contrast-to-noise ratio of 19.6 and 22.7 respectively. 14:30 3747. In Vivo SWIFT Imaging of SPIO Labeled Stem Cells Grafted in the Heart Rong Zhou1, Djaudat Idiadilitum2, Curt Corum2, Hualei Zhang1, Jia Zhong1, Hui Qiao1, Steen Moeller2, Michael Garwood2 1Department of Radiology, University of Pennsylvania, Philadelphia, PA, United States; 2Center for Magnetic Resonance Research, University of Minnesota, Minneapolis, MN, United States We demonstrate the first in vivo cardiac image by ECG-gated SWeep Imaging with Fourier Transformation (SWIFT). Myocardium anatomies are well-visualized on 3D SWIFT magnitude images. The positive contrast on SWIFT imaginary image facilitates the detection of SPIO-containing cells while the magnitude image provides anatomical reference without requirement for additional reference image. These data suggest that SWIFT might be an alternative to currently available positive contrast methods, attractive especially in cardiovascular applications. 15:00 3748. High-Resolution MR Angiogenesis Mapping with Integrin-Targeted Ultralow Gadolinium-Manganese Nanocolloids Dipanjan Pan1, Anne Schmieder1, Angana Senpan1, Shelton D. Caruthers1, Samuel A. Wickline1, Gregory M. Lanza1 1C-TRAIN and Division of Cardiology, Washington University in St. Louis, Saint Louis, MO, United States High-resolution MR Angiogenesis Mapping with Integrin-targeted Ultralow Gadolinium-Manganese Nanocolloids Yüklə 492,08 Kb. Dostları ilə paylaş:
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