Proposal for dbt builder programme
Group 4. Prof.K.Thyagaraju, Department of Biochemistry, SVUniversity, Tirupati
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Group 4. Prof.K.Thyagaraju, Department of Biochemistry, SVUniversity, Tirupati a. Rationale: The department of biochemistry, at present, is working on isolation, purification and analysis of various plant various parts products of Tirumala hills on the regulation of microbes, oxidant stress, cell induction, diabetes and obesity of animals. This department is conducting work on production of wine and fermentation to produce bye products as secondary metabolites for the human use. Further the department has established marker proteins for toxicity effect determination in animal systems and use of various plant products as antioxidants to these affected systems. In addition to these further conducting of experiments on cell biology, growth regulation of spermatozoa (rats), embryos (chick), and DNA and protein analysis of cells. Considering the significance work performed of Biochemistry department the following objectives are selected in this programme. b. Objectives:
c. Deliverables: The experimental output of the above objectives generates various pure phytomedicines which are used in curing the diseases of skin problems, liver, kidney, heart, pancreas, eye, brain and blood metabolic problems. They also serve as antibiotics to various microbes. The phytochemicals work at the level of induction of immune system, cell regeneration, and removal of abnormal parts like fat, dead cells etc. These phytomedicines can also generate finance to the producer as pharmaceuticals depending on the function for which they are used and made into the forms as tablets, caplets, capsules, syrups and powders. 20. Existing level of collaborations, if any with
21. Detailed Work Schedule, Time line & Targets (Year-wise):
22. Have you read the purpose of DBT scheme advertised in Newspapers/posted on the DBT WEBSITE? Through Web Site If yes, what transformation in future research, education and innovation you expect that the DBT support proposed in life sciences by your university can bring about or what is your vision in applying for the grant under this programme. Future research plan: Establishement of stem cell research centre, Development of clinical viral diagnostic laboratories, Development of digital data documentation and consevaration of rare and endangered species of plants and animals in Seshachalam forests, Development of drug research and docking laboratory. Education and Innovation expectation: Based on the outcome of the research results it is expected to propose an education centre on stem cells and their applications, development of drought tolerant plants and agricultural crops for the drought area of Rayalaseems region, to develop markers for various diseases and tools for rapid and low cost diagnostics of viral diseases and to produce various pharmacological products for the diagnized diseases of this region and their efficacy. Vision of the University: To strengthen the life sceicne courses of our university by modifying them into an advanced research centres with advanced equipment facilities to make an state of art laboratories to suit the applications of biotelchnology. 23. What are the expected limitations/risks in implementation in this programme in case of sanction of DBT proposal submitted by you?
Future limitations:
* Deduct the details of contribution from university and private industry. 24. Budget Particulars: BUDGET IN LAKHS Rs.
Total support sought from DBT: Rs. 2990.36lakhs Contribution from SVUniversity: Rs. 513.00lakhs The following Infrastructure will be provide to run the BUILDER Programme.
Approximate cost: Rs. 350.00 lakhs
and also first floor (1155 sft) Approximate cost of the building: Rs. 53.00 lakhs
AC sheets (4815 sft) Approximate cost: Rs. 35.00 lakhs
Detailed Justification of Budget item-wise: 1. State-of-the art Labs for Teaching 2. Common facilities 3. Equipment for Research (separate list may be enclosed) 1. State of the art labs for teaching and training: Stem Cell Biology Course and laboratory facility: SV is proposing to start M.Sc., Stem Cell Biology an interdisciplinary course which is essential to train the students for providing hands-on-skill experience to cater the needs of hospitals available in the drought prone area of Rayalaseema region which is useful to treat the patients suffering with various diseases/disorders. The Innovate course on Stem Cell Biology is essential for production of stem cells for animal models and study of regenerative capacity under in vivo conditions. The faculty and the man power proposed in the BUILDER proposal are necessary for both teaching and research on Stem Cells. The SVUniversity is also made an MoU with Nichi-Stem Cell Research Centre of Chennai, a branch of Japan which will help us to strengthen the course. This will be helpful to the university to strengthen the advanced research with multidisciplinary approach. The grant also be essential for modernization and establishment of digital and on line e class rooms, WiFi facility, e-governance of life science labs, which can be used to impart advanced training for young faculty, post-doctoral, doctoral and post graduate life sciences students of this campus and other institutes of this region. Requirements: Rs.600 lakshs
2. Common facilities to be required: The number of labs to be required under Builder programme: Rs.1200 lakhs 1. Cold room facility: Screening, isolation, identification of biomolecules. Rs. 50 lakhs Requirements: Cold room with illumination, humidity detectors, timers, working benches. 2. Animal cell culture laboratory: In vitro testing of the antimicrobial, anti-inflammatory and antibiotic efficacy of the biomolecules by using various animal cell lines and cultures. Requirements: CO2 incubator (air jacket), Inverted microscope, Biosafety level III cabinet, -800C vertical deep freezer, Temperature controlled Liquid Nitrogen container, FACS, Fluorescent microscope, Automated blood analyzer, Automated biochemical analyzer, Automated cell analyzer. Rs. 100 lakhs 3. Molecular biology laboratory: Isolation, characterization of genomes, proteins/enzymes and substrates. Rs. 100 lakhs Requirements: Gradient PCR machine, QPCR, PCR workstation, Microarray, In Situ hybridization high through out analyzer, High speed refrigerated table top centrifuge with variable rotors, Electrophoresis units, 2D electrophoresis unit and Blotting units- Western, Sothern, Hybridization oven, DNA cross linker, Speedvac, -800C vertical deep freezer, , -200C vertical deep freezer, Clean bench facility, Liquid handling facility, Gel doc system, Ultra-pure water system, HPLC/FPLC, Incubator shaker, Gel rockers, Sterilization facility- dry, wet and filter systems, Ultrasonicator. 4. Biotechnology lab: Gene expression studies of various bioactive molecules by using different cloning strategies. Rs. 150 lakhs Requirements: Gene gun/projectile, Vertical Laminar airflow unit, short nucleotide DNA sequencer, 37oCIncubator, Incubator shaker, Gradient PCR machine, Refrigerated Microfuge, Electrophoresis and Blotting units- Western, Sothern, Hybridization oven, DNA cross linker, Speedvac, -800C and -200C vertical deep freezers, Ultrasonicator, 5. Plant and tissue culture lab: Cultivation of various endangered species and in vitro synthesis of secondary metabolites, testing of plants for transgenic. Requirements: Tissue culture cabinet/chambers, Horizontal laminar flow bench, Dissection microscopes, Vertical microscope with high magnification, Microtomes, Incubators, Sterilization facility- dry, wet and filter systems. Rs. 50 lakhs 6. Virology laboratory: This laboratory is essential for isolation, cultivations, characterization and maintenance of various viruses and also to develop the immune and molecular based diagnostics. Requirements: Transmission Electron Microscope, DNA sequencer, Ultracentrifuge floor model, High speed refrigerated centrifuge with variable rotors, ELISA readers, Gel doc system, 37o C Incubator, Incubator shaker, Gradient PCR machine, Refrigerated Microfuge, Electrophoresis and Blotting units- Western, Sothern, Hybridization oven, DNA cross linker, Speedvac, -800C and -200C vertical deep freezers. Rs. 100 lakhs 7. Drug discovery and Pharmacology lab: The Drug discovery is useful to isolate molecules from various organisms and their analogues will be synthesized and prepared in the form of various medicinal constitutents. After the synthesis of all these molecules they will be tested using organzims like microbes, animals and humans in Pharmacology laboratory using the available tools of this laboratory. Rs. 100 lakhs Requirements: FTIR, LCMS, UV-Vis Sectrophotomter, HPLC, High speed centrifuge and other minor equipments. 8. Molecular modeling and Bioinformatics lab: In Silico modeling of lead molecules and study their functional capacity by using various bioinformatic tools. Rs. 100 lakhs Requirements: High speed computer systems- servers and various commercial soft wares and packages, data storage system, networking, online backup, highspeed internet facility. 9. Environmental toxicology laboratory: This lab is essential for analysis of environmental toxic compounds and their role on induction health disorders. Rs. 40 lakhs Requirements: Atomic absorption spectrophotometer, 10. Modernization of animal house facility: The existing animal house can be upgraded according to the animal ethical committee standards to cater the needs of the people who are working on animal models for various In vivo studies. Rs. 100 lakhs Requirements: Computer controlled temperature system, animal cages, Modern hygienic racks, Animal rearing systems, Animal slotter equipments. Yüklə 1,36 Mb. Dostları ilə paylaş:
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