Tez özetleri Astronomi ve Uzay Bilimleri Anabilim Dalı 2

Role of Galectın-1 on Dextran Sulphate Sodıum-Induced Colıtıs Model ın Mıce
Ulcerative colitis is an inflammatory bowel disease which is characterized by an effect on the mucosa and submucosal layer of the colon, peripheral and mucosal T-cell activation, the release of reactive oxygen metabolites from neutrophils and macrophages, and increase in the synthesis of pro-inflammatory cytokines. Galectin-1 (Gal-1) is a member of β-galactoside binding proteins which regulate T-cell mediated immune response and diverse biological processes such as cell growth, cell proliferation, cell migration, and wound healing.
The aim of this study was to reveal the role of Gal-1 on colon morphology, cell proliferation, oxidative stress, anti-oxidant system, inflammatory and anti-inflammatory mediators in the model of experimental acute colitis induced by dextran sulfate sodium (DSS) in mice.
A total of 32 adult male C57BL/6 mice used in this study were divided into four groups. I. group, control animals injected with PBS and given sterile tap water; II. group, mice administered orally 3 % DSS for 5 days to induce acute colitis; III. group, animals injected intraperitoneally with 1 mg/kg recombinant human Gal-1 once a day for 7 consecutive days; IV. group, mice both injected recombinant human Gal-1 and given 3 % DSS. During the experiment, change % in body weight of the mice and disease activity index (HAI) was determined in a daily manner. The effects of Gal-1 on DSS-induced colonic injury were determined using colitis scoring system by examining macroscopically and microscopically. Its role on cell proliferation was shown by Ki-67 immunohistohemistry. Parameters of anti-oxidant system such as glutathione (GSH), glutathione peroxidase (GSH-Px), catalase (CAT), superoxide dismutase (SOD), and malondialdehyde (MDA) level which is a marker of oxidative injury, were examined by spectrophotometry. Myeloperoxidase (MPO) levels as an indicator of leukocyte infiltration, tumor necrosis factor-α (TNF-α) and interleukin-10 (IL-10) levels in colon tissues determined by ELISA.
Administration of DSS resulted in a common injury in colon by causing degenerative changes, an increase in HAI, MDA, MPO and TNF-α levels; a decrease in bodily weight, colon length, cell proliferation index, GSH-Px, CAT and SOD activities, GSH and IL-10 levels. Gal-1 pretreatment prevented colonic injury by a significant reduction in HAI, MDA, MPO and TNF-α levels; an increase in body weight, colon length, cell proliferation, GSH-Px, CAT and SOD activities, GSH and IL-10 levels. As a result, this study showed that Gal-1 has proliferative, anti-oxidant, anti-inflammatory, and protective effects against DSS-induced acute colonic injury in mice. The results obtained from this study suggest that Gal-1 can be used as a therapeutic agent expected to contribute in preventing and treating of inflammatory bowel disease such as ulcerative colitis.

YILMAZ Öznur

Danışman : Doç. Dr. Füsun ÖZTAY

Anabilim Dalı : Biyoloji

Programı : Zooloji

Mezuniyet Yılı : 2014

Tez Savunma Jürisi : Doç. Dr. Füsun ÖZTAY

Prof. Dr. Şehnaz BOLKENT

Prof. Dr. Gül ÖNGEN

Prof. Dr. Cihan DEMİRCİ TANSEL

Prof. Dr. Kadriye AKGÜN DAR