Traditional Posters: Miscellaneous

Methodology for MRS of Cells, Body Fluids, etc.

Hall B Wednesday 13:30-15:30

919. Vision and Oxygen Inhalation Affect Mitochontrial Activity: A 31P Magnetic Resonance Spectroscopy Study

Ren-hua Wu^1,2, Hui Wang³, Poublanc Poublanc², Karel terBrugge², David Mikulis<sup>2

1</sup>Medical Imaging, Shantou University, Shantou, Guangdong, China; ²Medical Imaging, University of Toronto, Toronto, ON, Canada; ³Learning Science Center, Southeast University, Nanjing, China

Our aim was to know the relationship between oxygen inhalation and mitochondrial activity, and relationship between vision and mitochondrial activity as well. Eleven healthy volunteers underwent 31P MRS examination. ¦ÃATP, ¦ÁATP, ¦ÂATP, and ¡°potential of hydrogen¡± (pH) were measured. We can observe increased ¦ÃATP, ¦ÁATP, and ¦ÂATP peaks on the 3rd scan breathing hyperoxic air with eyes opening. Brain mitochondrial activities were increased and more ATPs were produced after oxygen inhalation in healthy volunteers. More energy is needed in visual status.

920. Bacteria-Specific Biomarkers in Mouse-Models of Infections Investigated by NMR Spectroscopy

Verena Hoerr¹, Lori Zbytnuik², Paul Kubes², Hans Vogel<sup>1

1</sup>Department of Biological Sciences, University of Calgary, Calgary, Alberta, Canada; ²Department of Physiology and Biophysics, University of Calgary, Calgary, Alberta, Canada

In mouse-models of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa infections, serum was investigated by 1H NMR spectroscopy and distinguished by statistical pattern recognition techniques. By combining the results of the in vivo study with footprints of culture experiments, potential bacteria-specific biomarkers were identified. We also compared serum metabolite changes caused by lipopolysaccharides (LPS) treatment and E. coli infection in both wild-type and Toll-like receptor 4 (TLR4) deficient mice. In TLR4 deficient mice the immune response upon LPS treatment was suppressed. Taken together, our approach allows us to distinguish between innate immune and direct bacterial effects during an infection.

921. In Vivo Metabolic Analysis of Pseudomonas Aeruginosa Live Bacteria Using High Resolution Magic Angle Spinning NMR Spectroscopy

Valeria Righi^1,2, Caterina Constantinou³, Meenu Kesarwani³, Laurence G. Rahme³, A Aria Tzika<sup>1,2

1</sup>NMR Surgical Laboratory, Department of Surgery, Massachusetts General Hospital and Shriners Burns Institute, Harvard Medical School, Boston, MA, United States; ²Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Athinoula A. Martinos Center for Biomedical Imaging, Boston, MA, United States; ³Molecular Surgery Laboratory, Department of Surgery, Massachusetts General Hospital and Shriners Burns Institute, Harvard Medical School, Boston, MA, United States

We tested the feasibility of H1 High Resolution Magic Angle Spinning (HRMAS) NMR in determining metabolic profiles of live bacteria. We used Pseudomonas aeruginosa, a human opportunistic pathogen responsible for chronic and acute infections, and a major cause of morbidity and mortality in cystic fibrosis patients. We found that HRMAS is powerful technique for monitoring the metabolic fingerprint of in vivo models, including live bacterial cells. This technique may prove to be a helpful tool in gene function validation, the study of pathogenesis mechanisms and the testing of anti-bacterial agents.

922. Metabolic Aspects of N-3 PUFAs Supplementation to Rat Cardiomyocytes: A HR-MAS NMR and GC/MS Study

Valeria Righi^1,2, Mattia Di Nunzio^1,3, Francesca Danesi^1,4, Elisa Boschetti^1,4, Luisa Schenetti², Adele Mucci², Alessandra Bordoni^1,4, Vitaliano Tugnoli<sup>1

1</sup>Dipartimento di Biochimica "G. Moruzzi", Universita' di Bologna, Bologna, Italy; ²Dipartimento di Chimica, Universita' di Modena, Modena, Italy; ³Nutrition Research Center , Bologna, Italy; ⁴Nutrition Research Center, Bologna, Italy

Cardiovascular diseases (CVD) are responsible for significant morbidity and mortality throughout the world. We present a first investigation using HR-MAS NMR Spectroscopy in combination with GC/MS of neonatal rat cardiomyocytes supplemented with two different PUFAs, EPA and DHA, in order to understand the metabolic change occurring in these cells following the increase of their n-3 PUFA content. EPA and DHA are of special importance for human health, and fish oil feeding has been associated to reduced mortality in several studies.

923. A Metabonomic Analysis of Serum from Wilson’s Disease Rats Using ¹H NMR Spectroscopy and Pattern Recognition