Webmaster's Note: As Gerard says below, his Fourth Report came in two parts



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Frame = -3
Query: 543 VHEPPVQPDRCALSGNYRLESNPVRHDLSPLAAATGNRISRARYVGGATEFLKWWPNYGY 364

+HEPPVQPDRCALSGNYRLESNPVRHDLSPLAAATGNRISRARYVGGATEFLKWWPNYGY

Sbjct: 1 MHEPPVQPDRCALSGNYRLESNPVRHDLSPLAAATGNRISRARYVGGATEFLKWWPNYGY 60
Query: 363 TRRTVFGICALLKPVTFGKRVGSS 292

TRRTVFGICALLKPVTFGKRVGSS

Sbjct: 61 TRRTVFGICALLKPVTFGKRVGSS 84

--------------------

gnl|ti|818412214 name:POAA-aab33c07.b1

mate_pair:818411870

(881 letters)

Score E


Sequences producing significant alignments: (bits) Value

gb|AAB49621.1| vacuolar proton ATPase 100-kDa subunit [Dict... 77 8e-013

ref|XP_629892.1| vacuolar ATPase transmembrane subunit [Dic... 77 8e-013

emb|CAJ08612.1| vacuolar proton-ATPase-like protein, putati... 66 1e-009

ref|XP_814777.1| vacuolar proton translocating ATPase subun... 66 2e-009

gb|EAN95417.1| vacuolar proton translocating ATPase subunit... 66 2e-009

ref|XP_652779.1| vacuolar proton ATPase subunit, putative [... 65 3e-009

gb|AAX70459.1| vacuolar proton translocating ATPase subunit... 64 5e-009

ref|XP_653563.1| vacuolar proton ATPase subunit, putative [... 63 1e-008

emb|CAJ05081.1| vacuolar proton translocating ATPase subuni... 61 4e-008

ref|NP_179736.1| ATPase [Arabidopsis thaliana] >gi|20466448... 59 3e-007

>gb|AAB49621.1| vacuolar proton ATPase 100-kDa subunit [Dictyostelium discoideum]

Length = 815
Score = 77.0 bits (188), Expect = 8e-013

Identities = 38/87 (43%), Positives = 52/87 (59%)

Frame = +1
Query: 259 MSLGIFLSLLNYMKEKPPLNWINTLFQFIPQIVFLWCIFGYMCVLIVIKWCTDWGNGPCM 438

MS+GI SLLNY+ +K P+ +N L QF+PQ++FLW IFGYM VLI++KW +

Sbjct: 544 MSVGICFSLLNYLNQKGPIKIVNILTQFVPQMIFLWSIFGYMSVLIILKWVVPY------ 597
Query: 439 LFPFPLSFPLPPPPPPFPLPPCL*KFM 519

SF + PPF LP + F+

Sbjct: 598 -----RSFEVDKVDPPFILPTIIAMFL 619

------------

gnl|ti|818411870 name:POAA-aab33c07.g1

mate_pair:818412214

(927 letters)

Score E


Sequences producing significant alignments: (bits) Value

ref|XP_653563.1| vacuolar proton ATPase subunit, putative [... 52 2e-005

gb|EAN97434.1| vacuolar proton-ATPase-like protein, putativ... 52 2e-005

gb|AAM94005.1| vacuolar proton ATPase 100 kDa subunit [Grif... 52 3e-005

ref|XP_791302.1| PREDICTED: similar to Vacuolar proton tran... 52 4e-005

emb|CAB16306.2| Hypothetical protein C26H9A.1 [Caenorhabdit... 51 7e-005

ref|XP_814777.1| vacuolar proton translocating ATPase subun... 51 7e-005

gb|EAN95417.1| vacuolar proton translocating ATPase subunit... 51 7e-005

ref|XP_708125.1| PREDICTED: similar to ATPase, H+ transport... 50 9e-005

ref|XP_708124.1| PREDICTED: similar to ATPase, H+ transport... 50 9e-005

ref|XP_708123.1| PREDICTED: similar to ATPase, H+ transport... 50 9e-005

>ref|XP_653563.1| vacuolar proton ATPase subunit, putative [Entamoeba histolytica

HM-1:IMSS]

ref|XP_648633.1| vacuolar proton ATPase subunit, putative [Entamoeba histolytica

HM-1:IMSS]

gb|EAL48176.1| vacuolar proton ATPase subunit, putative [Entamoeba histolytica

HM-1:IMSS]

gb|EAL43247.1| vacuolar proton ATPase subunit, putative [Entamoeba histolytica

HM-1:IMSS]

Length = 803


Score = 52.4 bits (124), Expect = 2e-005

Identities = 34/92 (36%), Positives = 48/92 (52%)

Frame = -2
Query: 344 NKRTFRETYKLALCHAKIFYKIFVGHLSPFVHLRVIYLISLMNTKSFIIIMKKEVNFFCK 165

N ++ + L+L HA++ S F+ V YL+ MN I +

Sbjct: 704 NTASYLRLWALSLAHAQLG--------SVFLE-NVFYLLMEMNIFITIFV---------- 744
Query: 164 AYSVWAAATLAVLLVMETLSAFLHALRLHWYE 69

++VWA TLA+L+ ME+LSAFLH LRLHW E

Sbjct: 745 GFAVWALITLAILIGMESLSAFLHTLRLHWIE 776

-------------------

gnl|ti|818412215 name:POAA-aab33c08.b1

mate_pair:818411871

(902 letters)

Score E


Sequences producing significant alignments: (bits) Value

gb|EAL20548.1| hypothetical protein CNBE4680 [Cryptococcus ... 84 7e-015

gb|AAW43854.1| conserved hypothetical protein [Cryptococcus... 84 7e-015

ref|NP_082810.1| stromal membrane-associated protein 1 isof... 82 3e-014

dbj|BAB02056.1| unnamed protein product [Arabidopsis thaliana] 80 1e-013

ref|NP_188393.1| DNA binding [Arabidopsis thaliana] 80 1e-013

gb|AAG17004.1| ARF GAP-like zinc finger-containing protein ... 79 3e-013

gb|AAM67219.1| ARF GAP-like zinc finger-containing protein ... 79 3e-013

dbj|BAD31272.1| ARF GAP-like zinc finger-containing protein... 79 3e-013

ref|NP_568807.1| DNA binding [Arabidopsis thaliana] >gi|231... 79 3e-013

ref|XP_643438.1| hypothetical protein DDB0167328 [Dictyoste... 78 5e-013

>gb|EAL20548.1| hypothetical protein CNBE4680 [Cryptococcus neoformans var.

neoformans B-3501A]

Length = 438


Score = 84.0 bits (206), Expect = 7e-015

Identities = 36/43 (83%), Positives = 40/43 (93%)

Frame = -2
Query: 472 RWASTNLGIFICIRCSGIHRSMGTHISKVKSVSLDKWTPEQVE 344

RWAS NLG+F+CIRCSGIHRSMGTHISKVKS+ LD WTPEQ+E

Sbjct: 38 RWASWNLGVFLCIRCSGIHRSMGTHISKVKSIDLDIWTPEQME 80

--------------

gnl|ti|818412233 name:POAA-aab33e04.b1

mate_pair:818411890

(928 letters)

Score E


Sequences producing significant alignments: (bits) Value

dbj|BAC90287.1| gll2346 [Gloeobacter violaceus PCC 7421] >g... 68 5e-010

gb|AAV95560.1| conserved hypothetical protein [Silicibacter... 56 2e-006

ref|ZP_00767807.1| conserved hypothetical protein [Chlorofl... 56 2e-006

ref|ZP_00620693.1| hypothetical protein RoseDRAFT_3107 [Sil... 51 7e-005

ref|NP_104213.1| hypothetical protein mlr3010 [Mesorhizobiu... 50 9e-005

ref|ZP_00555281.1| conserved hypothetical protein [Jannasch... 50 9e-005

gb|EAL90718.1| conserved hypothetical protein [Aspergillus ... 46 0.002

emb|CAF32095.1| hypothetical protein, conserved [Aspergillu... 46 0.002

ref|ZP_00376799.1| hypothetical protein ELI2040 [Erythrobac... 44 0.011

gb|EAA73942.1| hypothetical protein FG05679.1 [Gibberella z... 42 0.023

>dbj|BAC90287.1| gll2346 [Gloeobacter violaceus PCC 7421]

ref|NP_925292.1| hypothetical protein gll2346 [Gloeobacter violaceus PCC 7421]

Length = 295


Score = 67.8 bits (164), Expect = 5e-010

Identities = 31/74 (41%), Positives = 46/74 (62%)

Frame = +3
Query: 189 LLTAGCVCFPSKWNVREKFMKDLPLVHQPVPPFGKHLANPVMEKLLQLKADSPIWRANWS 368

+L+A CVCFPS+W + EK L +H PVP + + LA PV +L+ ++P+WR NW+

Sbjct: 132 ILSAACVCFPSRWLLTEKVGLLLTGIHAPVPGYAEQLAGPVDRFFERLRVETPMWRINWT 191
Query: 369 ILDDLEGPDSLYLP 410

I+D P+ LP

Sbjct: 192 IVD---SPELFLLP 202

Score = 44.3 bits (103), Expect = 0.006

Identities = 20/38 (52%), Positives = 26/38 (68%)

Frame = +2


Query: 527 SADQVGDRVMYRVERQTLTRLPKSNAILFTIRTYQRPL 640

+A +R+ R+ERQTL RL +S +LFTIRTY PL

Sbjct: 212 TAQDAAERLWIRIERQTLRRLAESRTVLFTIRTYVHPL 249

-----------

gnl|ti|818412240 name:POAA-aab33e11.b1

mate_pair:818411897

(901 letters)

Score E


Sequences producing significant alignments: (bits) Value

ref|XP_794415.1| PREDICTED: similar to CG10222-PA [Strongyl... 72 3e-011

gb|AAH83538.1| Hypothetical protein LOC407722 [Danio rerio] 70 8e-011

ref|NP_999966.1| ATP binding domain 1 family, member B [Dan... 70 8e-011

ref|XP_466196.1| putative purine nucleotide binding protein... 70 1e-010

ref|XP_417724.1| PREDICTED: similar to hypothetical protein... 70 1e-010

gb|AAH41519.1| LOC398460 protein [Xenopus laevis] 69 2e-010

gb|AAH91618.1| MGC97781 protein [Xenopus tropicalis] >gi|71... 69 2e-010

gb|AAH80422.1| LOC398460 protein [Xenopus laevis] 69 2e-010

emb|CAG00035.1| unnamed protein product [Tetraodon nigrovir... 69 2e-010

gb|ABA40377.1| SJCHGC09445 protein [Schistosoma japonicum] 68 4e-010

>ref|XP_794415.1| PREDICTED: similar to CG10222-PA [Strongylocentrotus purpuratus]

Length = 308
Score = 72.0 bits (175), Expect = 3e-011

Identities = 32/56 (57%), Positives = 43/56 (76%)

Frame = -3
Query: 356 KISVVHLVDSYYCSSATNYVSVLLLTLSTMLQLEMPHVNVLSKIDLLTQYSNLGMH 189

K+ VHLVD++YCS ++ VLL +LSTMLQ+E+PHVN+LSKIDL+ QY L +

Sbjct: 130 KLVSVHLVDAHYCSDPAKFIGVLLTSLSTMLQMELPHVNLLSKIDLVEQYGRLAFN 185

-----------------

gnl|ti|818411925 name:POAA-aab33h04.g1

mate_pair:818412267

(895 letters)

Score E


Sequences producing significant alignments: (bits) Value

sp|P03851|YPB3_ECOLI Hypothetical 9.4 kDa protein 120 6e-026

gb|AAB59124.1| 10.2 kd ORF [Plasmid ColE1] >gi|9507257|ref|... 119 2e-025

ref|ZP_00374729.1| conserved hypothetical protein [Wolbachi... 77 1e-012

pir||JQ1541 hypothetical 16.9K protein - Salmonella typhimu... 52 4e-005

dbj|BAC04601.1| unnamed protein product [Homo sapiens] 49 2e-004

ref|XP_499069.1| PREDICTED: hypothetical protein XP_499069 ... 47 0.001

gb|AAS38609.1| hypothetical protein [Dictyostelium discoideum] 45 0.003

pir||A53577 ascites sialoglycoprotein 1 - rat (fragments) 45 0.003

ref|XP_645129.1| hypothetical protein DDB0217043 [Dictyoste... 45 0.003

gb|AAA85523.1| Heterodimeric complex composed of a mucin su... 45 0.003

>sp|P03851|YPB3_ECOLI Hypothetical 9.4 kDa protein

Length = 84
Score = 120 bits (301), Expect = 6e-026

Identities = 56/60 (93%), Positives = 58/60 (96%)

Frame = -3
Query: 707 RHDLSPLAAASGNRISRARYVGGATEFLKWWPNYGYTRRTVPGICVLLKPVSFGKRVGSS 528

RHDLSPLAAA+GNRISRARYVGGATEFLKWWPNYGYTRRTV GIC LLKPV+FGKRVGSS

Sbjct: 25 RHDLSPLAAATGNRISRARYVGGATEFLKWWPNYGYTRRTVFGICALLKPVTFGKRVGSS 84
Score = 49.3 bits (116), Expect = 2e-004

Identities = 24/41 (58%), Positives = 26/41 (63%)

Frame = -1
Query: 775 DPPVQPDRCALSGNYRLESNPGIDTTYRHWQQPVVTGLAER 653

+PPVQPDRCALSGNYRLESNP RH P+ R

Sbjct: 3 EPPVQPDRCALSGNYRLESNP-----VRHDLSPLAAATGNR 38

-------


gnl|ti|818412269 name:POAA-aab33h06.b1

mate_pair:818411927

(897 letters)

Score E


Sequences producing significant alignments: (bits) Value

emb|CAD37030.1| probable xanthine dehydrogenase [Neurospora... 49 2e-004

gb|AAT81740.1| xanthine dehydrogenase, putative [Oryza sati... 45 0.003

ref|XP_688983.1| PREDICTED: similar to xanthine dehydrogena... 45 0.003

ref|NP_990458.1| xanthine dehydrogenase [Gallus gallus] >gi... 45 0.004

gb|EAA68516.1| hypothetical protein FG01561.1 [Gibberella z... 45 0.004

ref|NP_035853.2| xanthine dehydrogenase [Mus musculus] 44 0.006

dbj|BAE26022.1| unnamed protein product [Mus musculus] 44 0.006

dbj|BAE37907.1| unnamed protein product [Mus musculus] 44 0.006

ref|XP_579397.1| PREDICTED: hypothetical protein XP_579397 ... 44 0.006

pdb|1WYG|A Chain A, Crystal Structure Of A Rat Xanthine Deh... 44 0.006

>emb|CAD37030.1| probable xanthine dehydrogenase [Neurospora crassa]

Length = 1364
Score = 48.9 bits (115), Expect = 2e-004

Identities = 21/27 (77%), Positives = 22/27 (81%)

Frame = -1
Query: 756 GTKLTCGEGGCGACTVEASIFDPTLNK 676

GTKL CGEGGCGACTV S F+PT NK

Sbjct: 63 GTKLGCGEGGCGACTVVVSQFNPTTNK 89

--------------

gnl|ti|820216905 name:POAA-aab46a05.b1

mate_pair:818409664

(420 letters)

Score E


Sequences producing significant alignments: (bits) Value

gb|AAO52506.1| similar to vacuolar Protein Sorting; Vps13p ... 62 6e-009

ref|XP_644147.1| hypothetical protein DDB0217546 [Dictyoste... 62 6e-009

ref|XP_637643.1| hypothetical protein DDB0187116 [Dictyoste... 40 0.020

ref|XP_663183.1| hypothetical protein AN5579.2 [Aspergillus... 39 0.075

gb|EAL89636.1| vacuolar protein sorting-associated protein ... 37 0.28

dbj|BAA25068.1| XA1 [Oryza sativa] 35 0.83

ref|XP_473935.1| OSJNBb0085C12.12 [Oryza sativa (japonica c... 34 1.4

emb|CAB73522.1| hypothetical protein Cj1268c [Campylobacter... 34 1.4

ref|YP_179390.1| oxidoreductase, FAD-dependent [Campylobact... 33 2.4

ref|NP_602106.1| putative integral membrane transport prote... 33 3.1

>gb|AAO52506.1| similar to vacuolar Protein Sorting; Vps13p [Saccharomyces

cerevisiae] [Dictyostelium discoideum]

Length = 2101


Score = 62.0 bits (149), Expect = 6e-009

Identities = 31/107 (28%), Positives = 50/107 (46%)

Frame = +3
Query: 48 YLKFRPKERPT*DPRAWWKYAGIKYDKIYFPFETCILDLLFPPICTLFPLSFSLSLIFI* 227

YLK+RPK+RP DP+AWWKY

Sbjct: 325 YLKYRPKQRPKQDPKAWWKY---------------------------------------- 344
Query: 228 NLPIGNVVIENIRDRLNRKSWVYLVQKSKIRNEYIQLYKSASGVKWV 368

+G+VV E+IR+++ ++SW ++ ++ + R EYI+L+K V W+

Sbjct: 345 ---VGDVVRESIREKIQQRSWTFIQKRRQNRKEYIKLFKKLQNVDWM 388

--------------

gnl|ti|820216907 name:POAA-aab46a07.b1

mate_pair:818409666

(907 letters)

Score E


Sequences producing significant alignments: (bits) Value

gb|AAO50779.1| similar to Mus musculus (Mouse). Sex-determi... 62 4e-008

ref|XP_642929.1| hypothetical protein DDB0217895 [Dictyoste... 62 4e-008

ref|XP_549834.1| putative tDET1 protein [Oryza sativa (japo... 53 1e-005

ref|NP_908360.1| putative tDET1 protein [Oryza sativa (japo... 53 1e-005

ref|XP_795871.1| PREDICTED: similar to de-etiolated homolog... 53 2e-005

emb|CAF91414.1| unnamed protein product [Tetraodon nigrovir... 53 2e-005

gb|AAH73728.1| MGC84545 protein [Xenopus laevis] 52 2e-005

gb|AAH76520.1| De-etiolated homolog 1 [Danio rerio] 52 2e-005

ref|XP_702074.1| PREDICTED: hypothetical protein XP_696982 ... 52 2e-005

gb|AAH55677.1| De-etiolated homolog 1 [Danio rerio] >gi|410... 52 3e-005

>gb|AAO50779.1| similar to Mus musculus (Mouse). Sex-determining region Y protein

(Testis-determining factor) [Dictyostelium discoideum]

Length = 1461


Score = 61.6 bits (148), Expect = 4e-008

Identities = 28/43 (65%), Positives = 34/43 (79%)

Frame = +1
Query: 292 VYDKRAFRRDYIPLAHHAGVYLYNDIFAVLSIQTQTIHLFQIQ 420

V DK F+RD I LAHH+GVYLY D+F VLS+Q Q IH+FQI+



Sbjct: 662 VRDKITFKRDVIHLAHHSGVYLYKDLFIVLSLQYQAIHIFQIK 704

----------------
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