Saccharomyces cerevisiae : Evoutions in Bio Sciences



Yüklə 445 b.
tarix27.10.2017
ölçüsü445 b.
#15375


Saccharomyces cerevisiae :


Evoutions in Bio Sciences

  • Ecology

  • Quantitative ecology

  • Physiology,

  • Quantitative biology

  • Systemic Biology

  • Holistic Biology





MICROBIAL/BIO REACTOR ENGINEERING:A BASIC TOOL FOR KNOWLEDGE IN HOLISTIC BIOLOGY

  • G.Goma,S Guillouet,C Jouve,J L Uribellarea

  • Laboratoire d ingenierie des systémes biologique et des procédés

  • UMR CNRS,INRA,INSA





Microbial Engineering:a part of biotechnologies

  • Find and improve the microorganisms for bio processing

  • Find the conditions of bio processing where the microrganism is economicaly performant

  • A multidisciplinary approach

  • A contraint : find the bottlenecks,eliminate them

  • An obligation:need of handling a complete tool box:from genes to bioproducts and bioprocess



What kind of technological strategy?

  • Low tech ?

  • High tech ?

  • Right tech for the goal



The IB Value Chain



The steps

  • Factory and his environment

  • The reactors ,biorector:biocatalist,srategy

  • Raw materials and biocatalist,bioreaction engineering

  • The biocatalist

  • Global implementation ;find the differents bottleneks and solve the problems

  • Need a tool box,and combining experimentals datas(strategy?) and simulations





Industrial (White) Biotechnology





Dual use of fermentors

    • RPM
    • Qair
    • Pressure
    • CO2, O2 ?
    • Gas balance
    • OD?
    • Ph (controlled)
    • Temperature
  • For this 2 controlled parameters, the analysis of the « work » of the control regulator gives informations









Metabolic descriptor

  • Mass conservation

  • Elemental biologicals reactions

  • Macroscopic kinetics

  • Matrix of reactions combining kinetics and stoechiometry of elemental reaction of metabolic pathways

  • Combining kinetics observed by on line measurements by robusts sensors evaluation of metabolics fluxes « on line » and nutritionals needs

  • Identification of some bottlenecks



Predictive modelisation and implementation of microbial processes





Prerequisite to “Systemic Biology”



Top Down strategy

  • Fit the macroscopic environnment,bioreactor

  • Find reproducible conditions:signature recognition

  • Biokinetics

  • Quantitative physiologie

  • Metabolic pathways

  • Proteomic

  • transcriptomics





Motivation



Identification and Classification of Physiological States

  • A bottlenek for « the omics »studies,for control strategies and « quality »

  • Morphometry

  • Kinetics and stoechiometrics « parameters »

  • Differentiation of biologicals and environmental effects



Yeast :Axenic culture gives a population production linked to some mechanims



The Tool box

  • Bio: the “omics”

  • +

  • Traditional technologies

  • +

  • mathematical tools

  • “the rule of innovation”





Microbial engineering is multidisciplinary : need of quantitative and “system” biology



Dual use of fermentors



Cell production kinetic



Cell and glucose ethanol concentration vs time (Fed batch with nutritional strategy)





Study of a reference fermentation



How does works are integrated ?



Studying the fast biological responses ...





The hyper yeast





Automate utilisé pour la supervision







Perspective : Use of behavioural modelling



Perspective : Use of behavioural modelling



Introduction

  • What is life

  • Diference beetwen an abiotic and biotic system

  • Ecosystem

  • Unicity and diversity of lives objects

  • Life by a whole,studies mainly on the parts

  • Remenber Z Zidane

  • Reductionism,integrativ biology,systemic biology, holistic biology



Bacteria

    • Bacteria
    • Yeast
    • Fungi
    • Eucaryotic cells
    • In every case
    • The basic law of biokinetics and stoechiometry are the same
    • But, every case have rules of utilisation with typical profile






Predictive modelisation and implementation of microbial processes





Study of a fermentation of reference



Study of a fermentation of reference





Heterogeneities:gradients(flux,stocks,,,) :microbe population



Top Down strategy

  • Fit the macroscopic environnment,bioreactor

  • Find reproducible conditions:signature recognition

  • Biokinetics

  • Quantitative physiologie

  • Metabolic pathways

  • Proteomic

  • transcriptomics



Synthesis



Basic concepts



Tackling complexity in industrial microbiology for bioprocessing








Yüklə 445 b.

Dostları ilə paylaş:




Verilənlər bazası müəlliflik hüququ ilə müdafiə olunur ©muhaz.org 2023
rəhbərliyinə müraciət

    Ana səhifə