Electronic Posters: Cardiovascular

Myocardial Function: Experimental & Human Studies I

Hall B Monday 14:00-16:00 Computer 29

14:00 3568. Transplantation of Murine Embryonic Stem Cell-Derived Cardiomyocytes Improves Cardiac Function in the Infarcted Heart

HuaLei Zhang¹, Hui Qiao¹, Nataliya Petrenko², Vickas Patel², Bin Huang³, Kenneth Boheler⁴, Victor Ferrari², Rong Zhou<sup>1

1</sup>Department of Radiology, University of Pennsylvania, Philadelphia, PA, United States; ²Dept of Medicine(cardiovascular), University of Pennsylvania; ³Department of Radiology, University of Pennsylvania, PA; ⁴National Institute of Aging, NIH

Highly enriched embryonic stem cell derived cardiomyocytes (ESC-CMs) were obtained in large numbers for in vivo study that examined the potential of these cells in the treatment of myocardial infarction. Our data suggest that ESC-CM mediated a teratoma free myocardial repair with significant recovery of regional and global contractile function over the period of 2 months.

14:30 3569. Detecting Real-Time Regional Myocardium Strain Changes Using Fast Strain-Encoded (FSENC) MRI

Ahmed Amr Harouni¹, Tamer A. Basha¹, Monda L. Shehata², Nael Fakhry Osman<sup>1,2

1</sup>Electrical and computer Engineering, Johns Hopkins University, Baltimore, MD, United States; ²Department of Radiology, Johns Hopkins University, Baltimore, MD, United States

Many techniques have been proposed for real-time interactive cardiac MR imaging. However, most of these techniques are limited to only the anatomical information. In this work, we propose to use fast strain-Encoded functional imaging technique to acquire real-time images then use a fully automated segmentation algorithm to provide online anatomical and functional information of regional myocardial regions. This can be useful in real-time monitoring of cardiac strain changes during either stress test, valsalva exercise or interventional operations.

15:00 3570. Cine DENSE MRI with Dual Displacement Encoding

Xiao Chen¹, Alistair Young², Frederick H. Epstein, <sup>1,3

1</sup>Biomedical Engineering, University of Virginia, Charlottesville, VA, United States; ²Anatomy with Radiology, University of Auckland, Auckland, New Zealand; ³Radiology, University of Virginia, Charlottesville, VA, United States

The purpose of the present study was to develop a cine DENSE sequence with dual displacement encoding, where two distinct stimulated echoes with different displacement-encoding frequencies are simultaneously stored along the longitudinal axis, and where either can be recalled at any particular time to to better accommodate a temporal sequence of images with different amounts of displacement and deformation at different times. The sequence was implemented on a 1.5T scanner and was evaluated and demonstrated by using a deformable phantom and by imaging the hearts of normal volunteers.

15:30 3571. MRI Characterization of Cardiac Tissue Scaffold Materials in Vitro and in Vivo

Daniel James Stuckey¹, Hikaru Ishii², Aldo R. Boccaccini², Carolyn A. Carr¹, Judith A. Roether², Qi Zhi Chen², Hedeer Jawad², Damian J. Tyler¹, Nadire N. Ali², Kieran Clarke¹, Sian E. Harding<sup>2

1</sup>Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, Oxon, United Kingdom; ²National Heart and Lung Institute, Imperial College, London, United Kingdom

MRI was used to test three different scaffold materials designed for myocardial tissue engineering. Scaffold location, degradation and effect on cardiac function were measured in vivo at 1 and 6 weeks after grafting of scaffold onto infarcted rat hearts. The rigid TiO₂-PED scaffold induced microvascular occlusion and necrosis adjacent to the scaffold, resulting in reduced cardiac function by six weeks. The PGS scaffold was not detrimental to function, but MRI showed that the material degraded between 1 and 6 weeks in vivo. This study demonstrates the feasibility and importance of using MRI to optimise myocardial tissue engineering strategies.