Executive Summary

Appendix 1: Contact details and description of institutions registered in LEP ordered by country (as per 01.01.2008)

Company	Organisation	Phone	ZIP	City	Street	Country	Keywords	Description	Type
UNESCO Chair Biophys. & Mol. Neurobiol	Universidad Nacional del Sur	054 2914861201	8000	Bahia Blanca	C.C. 857	Argentina	cell-surface receptors / lipid-protein interactions / membranes / structure / dynamics	biochemistry and biophysics of lipids cell-surface membrane structure and dynamics lipid-protein interactions in nicotinic receptors lipids in embryonic development lipids in neuronal differentiation and survival lipids in retina lipids in plants lipids in reproductive system	scientific
Inst. Biophysics & X-ray Structure Research / Fct. Lipidomics	Austrian Academy of Sciences	++43-316-4120-323	A-8045	Graz	Schmiedlstrasse 6	Austria	lipid polymorphism, membrane biophysics, membrane mimetic, membranolytic peptides	Our research aims at the elucidation of the molecular mode of action of host defence peptides that affect cells by interacting in a non-specific manner with their membranes and not via specific receptors. An understanding of how these peptides distinguish between bacterial and mammalian cell membranes will allow the design of novel peptide antibiotics, which can selectively kill bacteria. Bacterial resistance to such antimicrobial peptides is less likely to occur owing to the nature of their target and fast killing kinetics. However, there has been evidence that resistance to antibiotics may also arise due to changes in lipid composition of their membranes. Therefore, the design of novel and effective antimicrobial peptides will only be possible, if the entire lipid spectrum of its membrane has been identified, which nowadays owing to recent advances in analytical instrumentation can be addressed both at the qualitative and quantitative level. Experimental approach - membrane biophysics (structural, thermodynamic and spectroscopic techniques)- lipid analysis of target membranes - peptide libraries	scientific
BIOCRATES Life Sciences	BIOCRATES Life Sciences	++43-512-579823-4216	6020	Innsbruck	Innrain 66	Austria	Metabolomics, Lipid quantitation, Bioinformatics	Targeted metabolomics Mass spectrometry Quantitation of phospho- and glycolipids, eicosanoids etc. Bioinformatics and biostatistics Biomarker discovery and validation	industrial
Institute for Genomics and Bioinformatics	Graz University of Technology	++43-316-873-5345	8010	Graz	Petersgasse 14	Austria	transcriptional regulation, microarrays, adipogenesis, lipotoxicity	Transcriptional regulation of lipotoxic pathways. Gene expression analysis of mouse models of lipid-associated disorders.	scientific
Institute for Molecular Biotechnology	Graz University of Technology	++43-316-873-4089	A-8010	Graz	Petersgasse 14/I	Austria	yeast lipids, sterols	Lipid transport in yeast The roles of sterols in membrane transport in yeast Sterol homeostasis in yeast	scientific
Institute of Biochemistry, Cell Biology Group	Graz University of Technology	[phone]	A-8010	Graz	Petersgasse 12/2	Austria	yeast, mitochondria, lipid particles, phospholipids, neutral lipids	The main subjects studied in our group are synthesis of lipids and their assembly into organelle membranes of the yeast Saccharomyces cerevisiae. The majority of yeast lipids are synthesized in the endoplasmic reticulum with some significant contributions of mitochondria, the Golgi and the so-called lipid particles. Other subcellular fractions, e.g. the plasma membrane, are devoid of lipid-synthesizing activities. Spatial separation of lipid biosynthetic steps and lack of lipid synthesis in several cellular membranes necessitate an efficient transfer of lipids from their site of synthesis to their proper destination(s). The maintenance of organelle lipid profiles requires strict coordination and regulation of biosynthetic and translocation processes. Specific aspects currently studied are the assembly of lipids into mitochondrial membranes and lipid homeostasis in this compartment, and dynamics of neutral lipid storage in lipid particles. Recently we started to extend our studies to organelles of Pichia pastoris as a basis for future research of protein expression in this biotechnologically important yeast. Enzymes and other proteins involved in the above mentioned processes are investigated using biochemical, cell biological and molecular biological methods.	scientific
Structural biology	Inst. of Chemistry	[phone]	8010	Graz	Heinrichstr. 28	Austria	membrane protein, conjugation, T4SS	We are working on the characterization of a typ IV-like secretion system (T4SS) from Gram positive bacteria. The system is encoded on the resistance plasmid pIP501. The tra region shows a modular organization and contains 15 ORFs, of which several have been predicted to encode for transmembrane or membrane asociated proteins. Our aim is the structure elucidation of the components essential for the conjugative DNA transfer.	scientific
Institute of Medical Technologies and Health Mangement	Joanneum Research	++433168762103	8036	Graz	Auenbruggerplatz 20/3	Austria	acyl-coA, mass spectrometry, interstitial fluid analysis	analysis methods based on GC-MS, HPLC-MS^2 and nano-HPLC-MS^2 analysis of interstitial fluid of humans and animals method validation according to GLP guidelines	scientific
Molecular Biology and Biochemistry,	Medical University Graz	[phone]	A-8010	Graz	Harrachgasse 21	Austria	cholesterol metabolism, nuclear receptors, atherosclerosis	Novel and already identified genes involved in lipid homeaostasis of different organs including liver, intestine, endothelial cells, macrophages-foam cells. Cholesterol efflux and cholesteryl ester hydrolases. Pathophysiology of athersoclerosis. Lipid metabolism in gneral. Lipidome of human plasma in health and diseases. Lipidome of enterocytes and cardiomycytes .	scientific
IMB Biochemistry - Yeast Genetics Group	University of Graz	++43 316 380 5487	A8010	Graz	Schubertstr. 1	Austria	yeast, fatty acid, membrane and lipid imaging	Lipid metabolism and membrane assembly in yeast, yeast as a model of lipid-associated disorders, regulation of fatty acid, triglyceride and phospholipid metabolism, high-resolution microscopy, implementation of novel imaging methods to investigate membrane and organelle stucture and dynamics in yeast, yeast lipidomics	scientific
Institute of Chemical Technologies and Analytics, Bio and Polymer Analysis	Vienna University of Technology	++43 1 58801 15160	A-1060	Vienna	Getreidemarkt 9/164	Austria	MALDi, ESI, multistage MS, plant lipids, human blood	Characterisation of lipid pattern in human blood during dietary treatment Characterisation of lipid pattern of industrial (as a renewable source of chemicals), pharamceutical and medical relevant plants Development of ultrafast and structurespecific MALDI and ESI mass spectrometric techniques for all classes of lipids	scientific
Core Facility for Mass Spectrometry	ZMF/Medical University Graz	++43 (316) 385-73005	8010	Graz	Stiftingtalstrasse 24	Austria	Mass Spectrometry, FT-MS, Glycerolipids, Sphingolipids	Development of mass spectrometric tools for the analysis of lipids (neutral and polar glycerolipids, sphingolipids, cholesterols) and lipid derived second messengers (e.g. eicosanoids) is a key research interest of our facility. This is reflected by participation in center grants such as SFB Lipotox and the technology based initiative Lipidomic Research Center (LRC) Graz. Currently we develop an analytical platform based on UPLC-FT-MS/MS for differential quantitation of the lipidome at the level of molecular species. Finally these data should enable researchers to establish up- and down regulated metabolic pathways for different sets of samples. If it is needed to provide absolute quantitative data for single species or a limited set of them, we have the possibility to quantify them in a targeted approach with LC-MS/MS.	scientific
Div. Pharmacology - Dep. Mol. Cell Biology	K.U.Leuven	++32-16-345801	B-3000	Leuven	Campus Gasthuisberg, Herestraat	Belgium	phytanic, pristanic, ceramide, sphingosine-phosphate	Breakdown of fatty acids/derivatives via alpha-oxidation and beta-oxidation, peroxisomal lipid metabolism, bioactive sphingolipid metabolism, mouse models related to peroxisomal disorders,	scientific
CMPG-PFI	Katholieke Universiteit Leuven	++32 16 32 96 88	3001	Heverlee	Kasteelpark Arenberg 20	Belgium	yeast, sphingolipid, ESI-MS	We are interested in sphingolipidomics in yeast. Using ESI-MS, we have optimized a method to determine and relatively quantify the three different classes of complex inositolphosphoryl-containing sphingolipids [i.e. IPC (inositolphosphoryl ceramide), MIPC (mannosyl inositolphosphoryl ceramide) and M(IP)2C (mannosyl diinositolphosphoryl ceramide] in S. cerevisiae [Aerts et al., 2006, FEBS Letters, 580(7):1903-7]. We are interested in technologies enabling quantification of sphingoid bases and ceramides in yeast membranes.	scientific
Laboratory for Experimental Medicine and Endocrinology (LEGENDO)	Katholieke Universiteit Leuven	++32-16-330533	3000	Leuven	Herestraat 49 bus 902	Belgium	cancer, lipid rafts, protein acylation	Our team is interested in the metabolic changes in cancer cells versus normal cells, and particularly in the marked increase in lipogenesis that is observed in nearly all cancer types. We 1. study the mechanisms that underly this increase (imvolvement of oncogenes, steroid hormones,...), 2. We examine the impact of these chnages on lipid profiles, lipid rafts and lipid-modified proteins, 3. We investigate the consequences of these changes for cancer cell biology (growth, metastasis,...), and 4. We explore the potential clinical applications (diagnosis, therapy).	scientific
Neuronal Differentiation Unit	VIB and Catholic University of Leuven	[phone]	3000	Leuven	Herestraat 49	Belgium	cholesterol lipid rafts senescence	We are interested in analysing the role of cholesterol in the cell senescence/survival process. We postulate that cholesterol reduction in membrane lipid rafts it might be a central effector in survival of senescent neurons, implying that brain cholesterol regulation could be crucial for death/survival equilibrium durin brain aging.	scientific
Laboratório de Bioquímica e Biologia Celular de Lipídios	Departamento de Bioquímica - ICBS- Universidade Federal do rio Grande do Sul	55-51-33-16-55-50	90.035-003	Porto Alegre	Rua Ramiro Barcelos 2600- anexo	Brazil	shingolipids, gangliosides, adipogenesis, lipogenesis	- Sphingolipid metabolism and their cellular functions in central nervous system and hematopoietic differentiation. - Adipogenesis and lipogenesis.	scientific
Research Institute, Boggs Laboratory	Hospital for Sick Children	1-416-813-5919	M5G1X8	Toronto	555 University Ave.	Canada	sulfatide, galactosylceramide, glycosynapse	Structural organization of glycosphingolipids and effect of length of fatty acid chain and hydroxylation of fatty acid, Lipid rafts and membrane domains in myelin and oligodendrocytes and role in signalling, Trans interactions between glycosphingolipid head groups and formation of a glycosynapse between apposed cell membranes, Role of phosphatidylinositides.	scientific
CIHR Group in Molecular and Cell Biology of Lipids	University of Alberta	1-780-492-2963	T6G 2S2	Edmonton	328 Heritage Medical Research Centre	Canada	atherosclerosis, obesity, neurodegeneration, lipids	Goals of our CIHR Group in Molecular and Cell Biology of Lipids (MCBL) * To enhance the knowledge and understanding of the metabolism, function and transport of mammalian lipids, lipid biosynthetic enzymes and transport proteins, and the regulation of the genes that encode these proteins * To facilitate the translation of discoveries into potential diagnostics and treatments of human diseases * To provide fundamental knowledge that will improve the health of Canadians and peoples throughout the world * To provide an environment that will enhance scholarly and scientific endeavors. The principal investigators * Luis B. Agellon, PhD, Associate Professor of Biochemistry * Gordon A. Francis, MD, Associate Professor of Medicine * Richard Lehner, PhD, Associate Professor of Pediatrics and Cell Biology * Dennis E. Vance, PhD, Professor of Biochemistry * Jean E. Vance, PhD, Professor of Medicine Our research program The MCBL Group research program is organized into four major themes: * molecular regulation of genes involved in lipid homeostasis * biochemistry of lipid-protein interactions * lipid compartmentalization and intracellular trafficking * lipid homeostasis in murine models The current major research projects are: * regulation of phosphatidylcholine metabolism * molecular and cell biology of phosphatidylserine metabolism * triacylglycerol synthesis * role of triacylglycerol hydrolase in triacylglycerol metabolism * function and metabolism of sterols in the liver * metabolism of lipids in the enterohepatic circulation * cellular lipid efflux and HDL formation * lipid homeostasis in neurons Our Core Resources Members of the MCBL Group contribute specific technical expertise and newly developed technologies which are shared with other projects and laboratories through our Core resources. * The Core Cell Culture Laboratory provides access to a variety of cell lines (including various derivatives of McArdle RH7777 cells expressing recombinant forms of enzymes important in the metabolism of lipids), and technical support for immunofluorescence and confocal microscopy studies. * The Core Metabolism and Physiology Laboratory provides access to genetically-modified mouse strains used by investigators in the MCBL Group. This laboratory also provides access to primary cell cultures, in conjunction with the Core Cell Culture Laboratory, and technical support for surgical procedures. * The Core Lipid Analysis Laboratory provides access to current, standardized and validated analytical procedures, and technical support for the detection and analysis of lipids and their metabolites. * The Core Training Program provides opportunities for qualified postdoctoral fellows and graduate students to participate in our integrated research program.	scientific
Lab 305	Institute of Genetics and Developmental Biology, Chinese Academy of Sciences	86-10-64889783	100101	Beijing	Datun Road,Chaoyang District	China	lipid, gene cloning, gene function characterization	I am interested in improvement of oilcrops, lipid synthesis related gene cloning and gene function characterization.	scientific
Biochemistry and Nutrition Group, Biocentrum-DTU	The Technical University of Denmark	[phone]	2800	Lyngby	Building 224, DTU	Denmark	LC/MS, animal experimental facilities, GC, TLC	Research areas/competencies related with lipidomics: Identification and quantification of triacylglycerols and phospholipids by LC/MS, separation and quantification of different phospholipids and lipid classes by NP-HPLC, separation and quantification of triacylglycerol molecular species by RP-HPLC, separation and purification of lipids by TLC and SPE, analysis of fatty acid profiles by GC, analysis of lipid regiostructures by partial degradation (Grignard degradation for triacylglycerols & phospholipase degradation for phospholipids) followed with GC, GC/MS, LC/MS, analysis of plasma lipids by Cobas Mira, quantification of cholesterol in different lipoproteins using HPLC. Other research activities: Lipid absorption and metabolism, application of stable isotope techniques and GC/C/IRMS in metabolic studies, effect of dietary fats on learning ability and visual function, bioavailability of n-3 fatty acids, applying enzymatic technology in studies on lipid modification including structured triacylglycerols and structured phospholipids, studies on new dietary lipids such as diacylglycerols and conjugated linoleic acids, interactions of lipids with other food components, lipoprotein oxidation, development of analytical methods, especially emphasized on separation techniques and mass spectrometry for analysis of lipids and lipid soluble vitamins, qualitative and quantitative analysis of carbohydrates. We possess extensive expertise in lipid biochemistry and lipid analysis, and we have the following facilities: Analytical instruments for lipid analysis of food and biological systems (GC, HPLC, TLC, GC/MS, LC/MS, GC/C/IRMS, Cobas Mira, Spectrophotometer) Animal experimental facilities	scientific
University of Helsinki	Department of Medicine	[phone]	00020	Helsinki	PO Box 700	Finland	liver insulin marker	Profiling of human serum in individuals with a fatty vs no-fatty liver in collaboration woth Matej Oresic.	scientific
Helsinki Biophysics & Biomembrane Group	Institute of Biomedicine	[phone]	FIN-00014 University of Helsinki	Helsinki	POB 63	Finland	lipids, drugs, proteins, amyloids	lipid phase behavior, bioactive lipids, lipid-protein interactions, drug-lipid interactions, cationic lipids in gene delivery, lateral organization of biomembranes	scientific
Institute of Biomedicine/Käkelä Group	University of Helsinki	++358-9-19125409	P.O. Box 63	Helsinki	Haartmaninkatu 8/P.O. Box 63	Finland	Lipid-protein interactions, Mass spectrometry, Ion channels, Fatty acids	-Interactions of lipid membrane and membrane proteins/ion channels -Cellular metabolism of lipids and fatty acids -Mass spectrometry of lipids	scientific
Institute of Biomedicine/Somerharju group	University of Helsinki	358-9-19125410	00014	Helsinki	Haartmaninkatu 8/PL 63	Finland	mass-spectrometry, trafficking, membrane,phospholipase	1)Lipid trafficking 2)Regulation of lipid composition of cells (lipid homeostasis) 3)Mode of action of phospholipases 4)Membrane structure, expecially lateral distribution of lipids 5)Atherosklerosis and other lipid related diseases 6)Development of MS tools for lipid analysis	scientific
Department of Biochemisty	University of Oulu	[phone]	FIN-90014	Oulu	POB 3000	Finland	mitochondria, peroxisomes, structural enzymology	Lipids and metabolic disorders, yeast lipids, fatty acid synthesis, membrane lipidomics, lipid metabolism, lipid metabolizing enzymes,	scientific
Quantitative Biology and Bioinformatics	VTT Technical Research Centre of Finland	++358-20-720-4491	FIN-02044 VTT	Espoo	Tietotie 2, P.O. Box 1500	Finland	systems biology, early markers, metabolomics, phenotype characterization	Animal model phenotype characterization using lipid (and general metabolite) profiling approaches - Bioinformatics method for integration of lipid profile information from mass spectrometry based approches with other levels of data such as gene expression and protein profiles - Clinical applications of lipidomics for early disease detection and disease progression studies, focus on diabetes	scientific
Biological Chemistry Laboratory	Biological Chemistry LaboratoryNational Institute for Agronomic Research (INRA)	++33-1-30815474	78850	Thiverval Grignon	BP 1	France	oil, oil bodies, A thaliana, Y lipolytica, B napus	We are interested in understanding the biology of oil bodies, with special emphasis on assembly and degradation. We are currently studying two organisms: - A. thaliana, a model for for oil crop. -- Y. lipolytica, yeast capable to use and transform lipids. We combine a multiscale approach going from: -the identification of oil bodies proteins (proteomics, enzymatic studies, immunochemistry) --the study of isolated proteins (purified of recombined) in solution (enzymology, interfacial properties using hanging drop methods), or within model interfaces (Langmuir balance experiment) --- the study of proteins within complete organelle (from wt or mutants organisms) ----the study of wt and mutant model organism. ----- transcriptomic analysis of yeasts genes expressed or repressed upon shift from glucose to oleic acid medium. -Our goals are - to identify all protein component involved in oil bodies biogenesis -- to understand their role and identify their activity ---to study the influence of oil body protein composition on lipid content (nature, amount) Principal collaborations: -Within France Dr. M Miquel, Dr. L Lepiniec, Seed Biology Laboratory, INRA, Versailles: Molecular biology of A. thaliana seeds, Dr JM Nicaud Molecular Genetic Laboratory, INRA CNRS, Grignon: Molecular biology of Y. lipolytica. Dr. M Axelos: BIA Laboratory, INRA, Nantes. Interfacial methods -Within European Union Prof. G Daum, Dr K Athensdaedt, technical University, Graz: Lipid particles from yeast Dr. S Papanikolaou, Agronomic University of Athens, Biotechnology of Oleaginous yeast	scientific
INSERM-538 (biomembranes)	CHU Saint-Antoine	33-01-40011340	75571	PARIS 12°	27 rue Chaligny	France	membrane, microdomains, traffic, phospholipids	Membrane heterogeneity and polarized cellular traffic. Cholesterol and sphingolipids enriched membrane microdomains Rotavirus traffic in Caco-2 cells. Synthesis of bile phospholipids	scientific
Laboratory of Enzymology at Interfaces and Physiology of Lipolysis	CNRS	33 4 91 16 41 34	13402	Marseille cedex 20	31 chemin Joseph Aiguier	France	lipases, TLC-FID, monolayers	Lipolytic enzymes and their use for the characterization lipids Production facilities, purification and kinetic characterization of several recombinan t lipases, including gastric and pancreatic lipases, pancreatic lipase-related proteins, hormone-sensitive lipase, microbial lipases, Plant phospholipase D, phospholipase A2, phospholipase A1, galactolipase Use of thin-layer chromatography coupled to flame ionization detection (TLC-FID, Iatroscan) for quantitative measurement of neutral and polar lipid species and their lipolysis products. Use of the monomolecular film technique for studying lipid-protein intercations and lipolytic enzymes kinetics. This technique allows measurement of lipolytic activity with very low amounts of substrate (g)and is therefore suitable for rare lipids.	scientific
CIML	CNRS INSERM	33 491269404	13288	marseille	parc sxcientific de luminy	France	abc transporters, abca1, malaria, flippase	lipid transport /flip by abac transporters of the A class lipidomic analysis of microparticles during malaria infection	scientific
Laboratoire de Biogenèse Membranaire	CNRS UMR 5200 Université V. Segalen Bordeaux 2	[phone]	33076	Bordeaux cedex	146 Rue Léo Saignat	France	Plant lipids, waxes, fatty acids, GC, Lipid analysis	The Membrane Biogenesis Laboratory (MBL) has a long history of research in membrane biogenesis and lipid biosynthesis. This lab is internationally known and has been recently chosen to organise the International Plant Lipid Symposium in 2008. The aims of the research concern the identification and the characterisation of the genes involved in the regulation of lipid metabolism. Using Arabidopsis thaliana as the main plant model, the topics currently developed in the unit are: i/ research project I: Genomics of epicuticular wax metabolism. The objective is to characterise the genes involved in the biosynthesis of epicuticular waxes in order to understand the regulatory mechanisms of the expression of genes, which control the composition of the wax layer in response to various stress conditions. ii/ research project II: Membrane biogenesis and homeostasis. This project aims to characterise the mechanisms controlling the membrane lipid composition, the lipid transfer between organelles and membranes, and the lipid composition of lipid rafts in relation to hormonal signalling. iii/ research project III: SNAREs, lipids and endomembrane dynamics. The objective is to study the role of SNAREs in membrane dynamics between the ER and the Golgi apparatus in the secretory pathway leading to the biogenesis of plasmalemma and the formation of lipid rafts through the secretory pathway. The MBL is one unit of the IFR 103 Integrative Biology which involves 6 labs covering the different areas of plant research (pathogenesis, biotechnology, agronomy), and technological platforms (imaging, lipidomic, metabolism, transcriptome). The MBL has in charge the lipidomic platform which consist of 3 GLC,1 GC/-MS 1 densitometer, 1 phosphor imager and 2 TLC spotters. This platform allows lipid and fatty acid analysis and lipid metabolism studies.	scientific
Institute of molecular and cellular pharmacology	CNRS UMR6097	++33-(0)4-93957733	06560	Valbonne	Sophia Antipolis- 660 route des lucioles	France	phospholipase A2, lipid mediator, cancer, inflammation	Eleven genes coding for secreted phospholipases A2 (sPLA2s, 14-18 kDa) and 2 types of receptors (M and N) have now been identified in mammalian tissues. sPLA2s and their receptors are found in several tissues, and their expression levels are increased in inflammation, associated diseases, different types of cancer, and neurodegenerative diseases. Although the biological functions of sPLA2s and their receptors are still ill-defined, some sPLA2s are mitogenic and/or apoptotic, pro-inflammatory and/or pro-tumoral, but also anti-tumoral, anti-bacterial, anti-viral, and anti-parasitic. At the molecular level, sPLA2s are most likely bifunctional proteins, acting as both enzymes and ligands for a variety of soluble and membrane proteins. sPLA2 enzymatic activity participates in the control of the rate-limiting step in the production of lipid mediators such as prostaglandins and leukotrienes which are involved in a myriad of biological effects. We have previously identified for the first time the M and N type receptors for sPLA2s using snake venom sPLA2s as ligands. More recently, we have cloned 7 of the 11 known mammalian sPLA2s, and we have shown that several of these enzymes are the natural ligands of the M-type receptor, suggesting that mammalian sPLA2s are true ligands for mammalian proteins. The main objective of our research program is to determine the function of sPLA2s, while continuing their molecular characterization. Our specific aims are : 1) To analyze the molecular properties of sPLA2s and to develop key tools to determine sPLA2 function (recombinant production, native structure, crystallization, enzymatic and binding properties, search of specific inhibitors, characterization of receptors, transgenic mice), 2) To analyze the tissue distribution of sPLA2s and their receptors in normal and pathological states in relation with the following item, 3) To study the role of sPLA2s in three major research areas : colorectal cancer, inflammation, and host defense against viruses, bacteria, and parasites.	scientific
INSERM U467/IFR94 Proteomics Platform	Faculté de Médecine René Descartes Paris 5	[phone]	75015	Paris	156 rue de Vaugirard	France	lipid rafts, eicosanoids, epithelium	Protein-lipid interactions, proteomics and lipidomics of lipid microdomains, and their relevance in physiopathology of epithelial cells. Regulation of inflammation in cystic fibrosis.	scientific
French Institute for Fats and Oils (ITERG)	Industrial Technical Center	[phone]	33600	PESSAC	rue Monge	France	nutrition bioavailability cancer CHD	Nutritional impact of lipids (in relation to diseases : CHD, cancer, obesity). Biomarkers studies (adipose tissue, plasma, erythrocytes).	industrial
IMBL-Lipid Signaling (UMR 585 INSERM / INSA-Lyon)	INSA-Lyon and CNRS / INSERM / Univ Lyon1	33-4-72 43 82 40	69621	Villeurbanne	20 Ave A. Einstein	France	Signaling , Fatty acids , Lipid mediators , Analysis	INSERM UMR 585 / INSA-Lyon is part of IMBL (Institute for Multidisciplinary Biochemistry of Lipids). This UMR works on lipid signaling, in blood, vascular cells and adipocytes, in the context of aging, atherosclerosis, diabetes and obesity. Lipids of interest are membrane phospholipids as reservoirs of polyunsaturated fatty acids which serve as precursors of eicosanoids and docosanoids. The production of those oxygenated metabolites through cyclooxygenase and lipoxygenase pathways is the main focus. The role of oxidative stress/lipid peroxidation in those pathways as well as their control by polyunsaturated fatty acids of nutritional value are of special interest. The sphingomyelin / ceramide / sphingosine / sphingosine-1-phosphate is also taken into consideration.	scientific
INSERM U671	INSERM	++33142346923	75006	Paris	15 rue de l ecole de medecine	France	adipocyte obesity lipid droplet	Synopsis of current research interest in the field of lipid droplet biology: We are working in the field of metabolic diseases associated with obesity in an INSERM unit in Paris (UMR 671). In these diseases, ectopic lipid storage is a key deleterious event at the whole body level, associated with grave metabolic complications. In this regard, we are interested in lipid trafficking in adipose cells specialized in the storage of fat. Caveolins have been found at the surface of lipid droplets, but their functional role in the dynamics of fat storage remains unknown. Since caveolins are highly expressed in adipocytes, and caveolin-KO mice have revealed a striking inability to store fatty acids from extracellular sources into their adipose tissue, we focused our research on the role of caveolins in lipid homeostasis. In a recently published paper (Le Lay et al., Traffic 2006), we have shown that adipocyte lipid droplet composition was dependent on caveolin expression and that exogenous cholesterol was able to induce caveolin targetting to these lipid droplets through a process sharing many features in common with caveolar endocytosis. We are now interesting in elucidating the dynamic relationship between cell surface and lipid droplets through caveolin trafficking, and identifying the adipocyte lipid species that can be stored through a caveolin dependent pathway. In this line, our future projects deal with the functional role of caveolins in lipid targeting to the fat storage compartment, the lipid droplet.	scientific
INSERM U586 (Obesity Research Unit)	INSERM,	33-5-62172956	31432	Toulouse	CHU Rangueil BP84225	France	lysophosphatidic acid, autotaxin, lysophospholipase D, adipocyte,	My group is interested in the regulations and the biological consequences of the release of lysophosphatidic acid (LPA) by adipocytes. We are particularly interrested in a lysophospholipase D (autotaxin) which appears to be the main source of LPA release by adipocytes but which could also be involved in synthesis of several other bioactive phospholipids. ATX expressio is adipose tissue is up-regulated in association with insulin-resistance and type 2 diabetes. In paralelle, we are also interrested in understanding the transduction pathways (particularly the receptors) involved in LPA actions on the different cell types surrounding adipocytes in the adipose tissue including preadipocytes, macrophages, endothelial cells.	scientific
IFR30 Lipid Analysis Platform	INSERM/Toulouse Genopole	33 (0)561779414	31024	Toulouse	CPTP Bat C, Hopital Purpan BP3028	France	neutral lipid, ceramide, bile acid, phytosterol	Our Lipid Analysis Platform belongs to the French Nationale Institute for Health and Medical Research (INSERM). It is located in the Federative Research Institute (IFR30) in Toulouse, France and is associated to the Toulouse Genopole. The platform created in 2003 proposes a number of lipid analysis using chromatographic methods, either adapted from the litterature or original. We are equiped with 3 gas chromatographs (GC) with FID detection, one GC coupled to a mass spectrometer and one liquid chromatograph (HPLC) coupled to a light scattering detector (LSD). We propose qualitative and quantitative analysis of lipid molecules following extraction from micro-samples (biological fluids, tissues, cells). We thus have access to the molecular species of the following lipids : - Neutral lipids including cholesterol and related sterols, diacylglycerol, cholesterol ester and triacylglycerol (on the basis of carbon number) - Free or total fatty acids - Sphingomyelines and usual ceramides - Phytosterols - Bilary acids (conjugated or free) - We are in progress to propose in the near future the analysis of major phospholipids classes, galactolipids and cerebrosides by HPLC-LSD. Analysis are proposed on the basis of a price per sample. Collaboration on specific projects is opened. Present ongoing projects include the fields of intestinal lipid absorption, cancer and apoptosis, lipoprotein metabolism, nervous system functionnning, lipid transduction	scientific
Microbiology and Molecular Genetic Laboratory, UMR2585	National Institute for Agronomic Research (INRA) and National Centre for Scientific Research (CNRS)	++33 130815450	F78850	Thiverval-Grignon	CBAI B.P. 01	France	yarrowia lipolytica, lipid accumulation, genetic	We are interested in understanding the degradation of hydrophobic substrates (alkanes, fatty acids, oils) by the oleaginous yeast Yarrowia lipolytica. We combine a multistage approach going from: - transcriptional analysis of gene expression during growth on hydrophobic substrates using Y lipolytica micro array, - the identification of peroxisomal and oil bodies proteins (proteomics), - the study of wild-type and mutants affected in HS utilisation, in beta oxidation, in lipid accumulation, ... Our goals are to identified genes involved in lipid accumulation (functional analysis, nature and amount of fatty acid accumulated), lipid and HS transport into different cell organelles. Principal collaboration: - within France Dr T Chardot, Biological Chemistry, INRA, Grignon (biochemistry of lipid bodies), Prof N Latruffe, Burgundy University, Dijon (peroxisome), MC C Molina-Jouve, INSA, Toulouse (Biotechnology of oleaginous yeast), - within European Union: Prof G Daum, Dr Athensdaedt, Technical University, Graz (lipid particles from yeast), Dr S Papanicolaou, Agronomic University of Athens (Biotechnology of oleaginous yeast).	scientific
Cyberlipid Center	Private	330467414365	34090	Montpellier	2 rue du colonel Marchand	France	database internet lipids	Construction and administration of an internet site devoted to all lipid aspects www.cyberlipid.org which is yet cited in your links page	scientific
Nutrition, Croissance et Cancer, INSERM E211	Université François Rabelais	[phone]	37044	TOURS	CHU Bretonneau	France	diet, cancer, lipidome, PUFA, CLA	In the field of cancer prevention through dietary lipids, we use the white adipose tissue lipid composition as a qualitative indicator of past dietary intake of lipids. Through a reappraisal of white adipose tissue data obtained recently in human and animal models, we individualized a composite lipid profile associated with a low risk of breast cancer. This profile has led us to elaborate a composite index indicative of the risk of breast cancer. This biomarker offers the opportunity to quantify the part due to modifiable dietary factors in the risk of breast cancer and complements the currently known risk factors for breast cancer. Bougnoux P, Giraudeau B, Couet C. Diet, cancer and the lipidome. Cancer Epidemiology, Biomarkers & Prevention 2006, 15(3):416-21.	scientific
University of Bourgogne	University	[phone]	21000	DIJON	6 Bd Gabriel	France	Diacylglycerols, MAP kinases, T cells, Macrosomia	Dietary polyunsaturated fatty acids (PUFA) have been classified into two categories, belonging to n-6 and n-3 families. These fatty acids are indispensable for animal cell to maintain its structure, fluidity and function. The polyunsaturated fatty acids (PUPA) of n-3 family have been considered as authentic immunosuppressors, however, their mechanisms of action in T-cell activation have not been well elucidated. T-cell activation involves a series of complex mechanisms from membrane receptor to gene transcription via the second messenger cascades. The main theme of our research is to elucidate the role of dietary PUPA of n-3 family in human T-cells activation which might be involved in the pathology of several diseases like diabetes and obesity. We are studying the interaction of these fatty acids with the second messenger cascade / cell signalling (MAP kinases, calcium signalling, protein kinases C & diacylglycerol).	scientific
Applied Biosystems	Applera Deutschland GmbH	[phone]	64293	Darmstadt	Frankfurter Strasse 129B	Germany	SOP, MS, LCMS, funding, TaqMan Assays	Co-Developement of Gene Expression Assays Standard Operating Procedures for MS Lipid Analysis Business developement / Product & Service developement in Lipidomics	industrial
AkdÄ	Arzneimittelkommission der deutschen Ärzteschaft	++4930400456500	10623	Berlin	Herbert-Lewin-Platz 1	Germany	Clinical effects of lipid lowering, outcome trials, statins, safety of statins	Clinical effects of lipid lowering, outcome trials, statins, safety of statins	scientific
Julius-v-Sachs-Institute for Biosciences, Pharmaceutical Biology	Biocenter, University of Wuerzburg	++49-931-888-6160	D-97082	Wuerzburg	Julius-von-Sachs-Platz 2	Germany	plant oxylipins, isoprostanes	We are interested in the functional analysis of plant lipids. There is a long track record on plant oxylipins of the jasmonate type. However more recently we came interested in oxidized lipids derived from non-enzymatic oxidation of plant membrane lipids. The current focus is on hydroxy fatty acids and prostaglandin-like plant isoprostanes termed phytoprostanes. Formation of these compounds in response to environmental stress conditions is intensively been investigated in cyanobacteria and plants. It has been shown by our group that oxidized lipids (including phytoprostanes)represent archetype signals that induce detoxification and defence responses in plants and cyanobacteria. Moreover oxidized plant lipids also have an pronounced effect on mammalian immune cells. In the future, a comprehensive analysis of plant oxidized lipids and their biological effects in vivo (cyanobacteria, plants) will be performed. The lab is equipped with three GC-MS (sector field MS and quadrupol MS instruments). More recently, an Nano-ESI-HPLC-Ion-Trap MS instrument for plant proteome analysis and a HPLC-Triple Quadrupol MS for plant lipidomics have been purchased.	scientific
Eicosanoids and Tumor Development	Deutsches Krebsforschungszentrum	49 6221 424506	D 69120	Heidelberg	Im Neuenheimer Feld 280	Germany	Lipoxygenase, ichthyosis, epidermal barrier	The long-term objective of the research group is to substantially contribute to the understanding of the role of eicosanoids, which perform important tasks as local signals coordinating the concerted activities of different cell types in tissues under normal and disease conditions with a special focus on cancer. As autocoids the biological activities of eicosanoids are primarily determined by their biosynthesis via the cyclooxygenase (COX) and the lipoxygenase (LOX) pathways. Accordingly, the expression and activity of COX and LOX are strictly regulated under normal conditions, transiently up- or down-regulated during irritation and tissue regeneration, and permanently deregulated during carcinogenesis in both humans and mice. The group’s experimental strategy is to establish expression and activity profiles of individual LOX in mouse and human biopsies in order to bridge the animal data with clinical investigations. The experimental models used for functional studies include phorbol ester-irritated or wounded mouse skin, the two-stage skin carcinogenesis approach, and transgenic mouse lines with targeted over-expression or deletion LOX genes in skin epidermis or other epithelia in vivo and epithelial cells in vitro. Our current work is focussed on analyses of molecular mechanisms involved in the functions of epidermis-type LOX in tissue homeostasis and disease-related deregulation of eicosanoid metabolism.	scientific
Deutsche Gesellschaft für Proteomforschung	DGPF	089-1897 9007	82152	Martinsried	Am Klopferspitz 18	Germany	Proteomics, proteome research	Proteomics	scientific
department of cellular and molecular pathology	DKFZ(German Cancer Research Center)	49-6221-424350	D-69120	Heidelberg	Im Neuenheimer Feld 280	Germany	glucosylceramide synthase,cerebrosidesulfotransferase,cell-specific deficiency,fertilify	Glycosphingolipid and immune function Glycosphingolipid and transgene mice	scientific
Dept. of Neonatology, Lipid Metabolism and Developmental Physiology Group	Eberhard Karls Universität Tübingen	#49-(0)7071-29-86377	72076	Tübingen	Calwerstr. 7	Germany	Phospholipids, in vivo metabolism, D9-choline, human	The Lipid Metabolism and Developmental Physiology Group focuses on clinical as well as on developmental and comparative aspects of the metabolism of individual (phospho)lipid molecular species. Main topics are: (1) the metabolism of pulmonary phospho- and other lipids, together with that of their hydrophobic and hydrophilic proteins, in relation to pulmonary maturation, ante- and perinatal diseases and the development of bronchopulmonary dysplasia. (2) the function of individual and characteristic phospholipid molecular species with respect to air-liquid interface function under dynamic conditions and to immunological differentiation of immune-competent cells in terminal lung tissues. (3) the metabolism and secretion of hepatic phospholipids and their role in the supply of peripheral organs with lipids and lipid precursors during postnatal development and at different clinical settings, where liver function is compromised. To investigate these issues we have performed a lipidomic approach to individual phospholipid molecular species composition and metabolism of pulmonary surfactant systems. This includes investigation across a wide spectrum of vertebrate species in relation to development and their differing parameters of pulmonary physiology, air-liquid interface dynamics and parenchymal structures (avian air capillaries, mammalian sacculi, alveoli and airways). Furthermore, it includes investigation of functional characteristics of individual phospholipid components for phagocyte differentiation and function as well as for surface tension function. We have furthermore studied the relation of pulmonary surfactant molecular composition to extrapulmonary interfaces, like that of the hydrophobic gastric barrier. To investigate phospholipid metabolism under different clinical conditions and during postnatal development we have developed and established strategies using stable isotope labelled precursors in vivo in human subjects as well as in animal models, combined with electrospray-ionization tandem mass spectrometric (ESI-MS/MS) analysis of hydrophilic phospholipid precursors and their synthesis products. We have furthermore established animal models suitable for the investigation of inflammatory and hereditary diseases and during development, where changes of pulmonary and/or hepatic phospholipid metabolism as well as that of lipid-derived mediators is prominent (bronchopulmonary dysplasia, hyperoxic lung injury, cystic fibrosis).	scientific
Albrecht-von-Haller-Institute for Plant Sciences/Plant Biochemistry	Georg-August-University	++49-551-39-5743	37077	Goettingen	Justus-von-Liebig Weg 11	Germany	Plant lipid metabolism, lipid peroxidation processes, oxylipins, β,-oxidation	A central aspect in lipid metabolism is lipid peroxidation. It is common to all biological systems, both appearing in developmentally and environmentally regulated processes. Products are hydroperoxy polyunsaturated fatty acids and metabolites derived there from collectively named oxylipins or eicosanoids in mammals. They may either originate from chemical oxidation or are synthesized mainly by the action of various highly specialized forms of lipoxygenases. We are analyzing the physiological function of oxylipins during the degradation of storage lipids in oilseed seedlings as well as the involvement of lipoxygenases in the interaction of plants with pathogenic fungi and bacteria. Another research focus is the ana- and catabolism of lipid peroxides by analyzing the biosynthesis of hydro(pero)xy polyenoic fatty acids, aldehydes, ketols and divinyl ethers by combined biochemical and analytical methods as well as by using a metabolomic approach (oxylipin profiling). The use of plants as bioreactors for biotechnological purposes is addressed by isolating new fatty acid desaturases, acyl transferases and double bond isomerases in order to introduce new functional groups in seed oils for nutritional and industrial purposes. In order to identfy bottle necks within these projects we use lipid profiling methods.	scientific
Albrecht-von-Haller-Institute for Plant Sciences/Plant Biochemistry	Georg-August-University	++49-551-39-5743	37077	Goettingen	Justus-von-Liebig Weg 11	Germany	Plant lipid metabolism, lipid peroxidation processes, oxylipins, β,-oxidation	A central aspect in lipid metabolism is lipid peroxidation. It is common to all biological systems, both appearing in developmentally and environmentally regulated processes. Products are hydroperoxy polyunsaturated fatty acids and metabolites derived there from collectively named oxylipins or eicosanoids in mammals. They may either originate from chemical oxidation or are synthesized mainly by the action of various highly specialized forms of lipoxygenases. We are analyzing the physiological function of oxylipins during the degradation of storage lipids in oilseed seedlings as well as the involvement of lipoxygenases in the interaction of plants with pathogenic fungi and bacteria. Another research focus is the ana- and catabolism of lipid peroxides by analyzing the biosynthesis of hydro(pero)xy polyenoic fatty acids, aldehydes, ketols and divinyl ethers by combined biochemical and analytical methods as well as by using a metabolomic approach (oxylipin profiling). The use of plants as bioreactors for biotechnological purposes is addressed by isolating new fatty acid desaturases, acyl transferases and double bond isomerases in order to introduce new functional groups in seed oils for nutritional and industrial purposes. In order to identfy bottle necks within these projects we use lipid profiling methods.	scientific
Department of Macromolecular Structure Research	GKSS Research Center	++49-4152-87-1291	21502	Geesthacht	Max-Planck-Str. 1	Germany	peptide antibiotics, membrane biophysics, scattering	interaction in or at membrane interfaces sensing of biophysical membrane properties by proteins modification of biophysical membrane properties by proteins, peptides or membrane active molecules peptide antibiotics, viral infectivitity	scientific
Institute of Biology/Molecular Biophysics	Humboldt-University Berlin	49 (0) 30 2093 8860	D-10115	Berlin	Invalidenstr. 42	Germany	lipid, transport, fusion, virus	The research is interdisciplinary ranging from structural biology, molecular and cell biology, virology, spectroscopy to the design and synthesis of biomolecule analogues. The topics of basic and applied research are protein-mediated fusion of membranes (viral fusion proteins), virus budding, lipid-trafficking in eukaryotic cells, protein-lipid interaction in membranes, and application of membranes in nanobiotechnology. Cell Entry and Budding of Enveloped Viruses: A focus of our research is an early step of cell entry of enveloped viruses. Enveloped viruses as influenza virus or HIV fuse with respective membranes to deliver their genome into the host cell. In our lab enveloped viruses are employed to study the molecular mechanism of protein-mediated virus-membrane fusion. We use various biophysical and cell biology methods to follow directly the fusion process and to identify structural intermediates mainly by fluorescence microscopy and spectroscopy. To understand the functioning of viral fusion proteins, specific mutants and chimeras of those proteins are created and probed for their fusion activity. By strong collaboration with groups specialised in electron microscopy and in image reconstruction, we identify the three dimensional structure of complete viral fusion protein at conditions typical for triggering membrane fusion. Another research focus is the assembly and budding of enveloped viruses. We are interested in mechanisms underlying local enrichment of viral components at the budding site of the host membranes. Lipid-trafficking in eukaryotic cells: Lipids play an essential role in various cellular processes including signal transduction. Our specific interest is the protein-mediated translocation of lipids across cellular membranes and their intracellular transport to distinct organelles. Although indirect evidence already exists the molecular identification of translocases pumping on the expense of energy (specific) lipids across membranes and of flippases facilitating a rapid, energy independent and unspecific movement of lipids in membranes is still awaiting. We are very much interested in the physiological relevance of those protein-mediated lipid transports and their function(s), for example in cell genesis, apoptosis, fertilisation, cell shape, exo- and endocytosis, in the enrichment of specific lipids in the bile fluid as well as in the mechanism and consequences of the lipid transport mediated by multi drug resistance proteins in tumor cells.	scientific
Microbial Expression Technology	Institute for Molecular Enzyme Technology, Heinrich-Heine University Duesseldorf	[phone]	52426	Juelich	Stetternicher Forst	Germany	lipolytic enzymes, biotechnology, biodetergents	- Biochemistry and biotechnology with lipases, esterases, phospholipases, thioesterases - Biodetergents, e. g. rhamnolipids from Pseudomonas aeruginosa - Membrane processes in pathogenic bacteria	scientific
LIPIDOMIX GmbH	LIPIDOMIX GmbH	++49-30-76778940	D-13088	Berlin	Berliner Allee 261-269	Germany	eicosanoids, phospholipids, HPLC, mass spectrometry	Analytic of eicosanoid related substances like: Prostaglandins Leukotrienes Hydroxy- and Epoxy fatty acids High throughput analytics Replacement of immuno assays and: Fatty acids Phospholipids, Sphingolipids Lipid profiling using: HPLC - Triple quad mass spectrometry	industrial
Walther-Straub-Institute	Ludwig-Maximilians-University	[phone]	80336	Munich	Goethestr. 33	Germany	inflammation, eicosanoids, obesity	Arachidonic acid metabolism, pharmacology of inflammation, neurotransmitter, obesity	scientific
Max Planck Institute of Molecular Plant Physiology/Plant Lipid Group	Max Planck Society	++49-331-567-8259	14476	Golm	Am Mühlenberg 1	Germany	galactolipid, tocopherol, phylloquinone	Biochemistry and Molecular Biology of Plant Lipids: Galactolipids, Tocopherol, Phylloquinone, Chloroplast Lipids, Phospholipids, Fatty Acids	scientific
Institute of Medical Physics and Biophysics	Medical Faculty, University of Leipzig	++49-341-9715733	04107	Leipzig	Härtelstrasse 16-18	Germany	MALDI, 31P NMR, Phospholipids, Lysophospholipids	We are using using MALDI-TOF mass spectrometry and 31P NMR spectroscopy for the evaluation of physiologicallly relevant phospholipids. Besides methodological work, we are particularly interested in - the activity determination of phospholipases and phosphoinositde kinase - the lipid / phospholipid composition of tissues and body fluids - the changes of the lipid patterns under inflammatory conditions (e.g. in rheumatoid arthritis and atherosclerosis) - the changes of the lipid pattern of cells during differentaition	scientific
Institute for Biology I, Botany	RWTH Aachen University	[phone]	52074	Aachen	Worringerweg 1	Germany	plant glycerolipid isoprenoid	our research focuses on plant lipid metabolism with special interest in formation/function of glycerolipids (glycerophospholipids) and isoprenoids.	scientific
Institute for Pathology, University Hospital Áachen	RWTH-Aachen	++492418089729	52074	Aachen	Pauwelstr. 30	Germany	acyl-CoA, ceramide, liver	Functional characterisation of acyl-CoA synthetase and its products, esp. in gastrointestinal tract and liver. Cellular localisation, concentration and function of acyl-CoAs and lipids.	scientific
Kekulé Institut f. Organische Chemie und Biochemie	Universität Bonn	++49 228 735346	53121	Bonn	Gerhard-Domagk-Straße 1	Germany	Sphingolipids, Glykosphingolipids, Sphingolipidoses, Lipid transfer proteins	The research of Konrad Sandhoffs group focuses on sphingolipids and sphingolipidoses. Metabolism of sphingolipids and glycosphingolipids, its function, regulation and topology are studied in vitro at the cellular and organismic level. Sphingolipid disorders are analyzed to clarify human diseases and their molecular and cellular pathogenesis. At the biochemical level, we synthesize sphingolipid probes and analogues to analyze their molecular environment in the cells, and to characterize enzymes and sphingolipid activator proteins involved in sphingolipid metabolism and membrane digestion at lipid-water interphases. We develop lipid transfer assays for the analysis of lipid transfer between membranes by transfer proteins.	scientific
Kekulé-Institute for Organic Chemistry and Biochemistry	University Bonn	++49-228-732703	53121	Bonn	Gerhard-Domagk-Str. 1	Germany	sphingosine-1-phosphate, ceramide-1-phosphate, neurodegeneration, postoperative ileus.	A)Sphingosine-1-phosphate signaling in terminally differentiated post-mitotic neurons and its implications for the pathology of neurodegenerative disorders. B)The pro-inflammatory functions of sphingosine-1-phosphate and ceramide-1-phosphate and its implications in postoperative ileus.	scientific
Department of Gastroenterology	University Heidelberg	([phone]	69120	Heidelberg	INF 410	Germany	Acyl-CoA synthetases, Lipid droplets, Rafts, inflammatory bowel disease	Acyl-CoA synthetases in fatty acid uptake Lipid droplet formation Lipid rafts in fatty acid uptake Lipid rafts in intestinal inflammation Lipid MS analysis of intestinal mucus	scientific
Insitute of Laboratory Medicine, Clinical Chemistry and Molecular Diagnostics	University Hospital Leipzig	++49-341-9722407	04103	Leipzig	Liebigstraße 27	Germany	phytosterols, oxysterols, eicosanoids	Sterol lipids: sterol metabolismn and atherosclerosis. Application of LC-MS/MS for the measuerment of free and esterified phtyosterols, lanosterol and cholesterol in serum and tissue. Eicosanoids: Evaluation of a LC-MS/MS method for the measurements of eicosanoids as marker of oxidative stress and inflammation.	scientific
Institute of Biochemistry I	University of Cologne	++49-221-4783656	50931	Cologne	Joseph-Stelzmann-Str. 52	Germany	lipid droplets, biosensor technology, membrane protein trafficking, endocytosis	Our interest covers the analysis of lipid droplet associated proteins and their function in lipid turnover. Coming from the field of membrane protein trafficking we apply cell based assay based on life cell imaging and conventional confocal immunofluorescence techniques to localize proteins. We further have a long-standing expertise in the analysis of biomolecular interaction analysis using surface-plasmon-based biosensors (BIAcore 3000, T-100). We analyse protein-protein interactions as well as membrane protein interactions by this very sensitive method.	scientific
Biocenter Klein Flottbekand Botanical Garden/Plant Physiology	University of Hamburg	[phone]	D-22609	Hamburg	Ohnhorststr. 18	Germany	sphingolipid, desaturase, glycosyltransferase	Molecularbiology and biochemistry of plant and fungal lipids: Functional characterizations of sterol-, sphingolipid and glycerol lipid-modifying enzymes such as glycosyltransferases and desaturases/hydroxylases.	scientific
Institut of Nutrition	University of jena	++49-03641-949610	D-07743	Jena	Dornburger Str. 24	Germany	metabolism, CLA, omega-3, sterol	fatty acid metabolism, CLA, omega-3 LC-PUFA, sterol metabolism	scientific
Institut for Food Chemistry	University of Muenster	[phone]	48149	Muenster	Corrensstr. 49	Germany	HPLC-MS/MS fumonisin sphingolipids food ceramide synthase	HPLC-MS/MS analysis of sphingolipids Influence of fumonisins on the sphingolipid metabolism	scientific
Leibniz-Institute of Arteriosclerosis Research	University of Münster	[phone]	48149	Münster	Domagkstr. 3	Germany	coronary heart disease, biomarkers, NMR, mass spectrometry	Research on biomarkers related to chd, stroke, Alzheimer disease risk. Specific interest in technologies: H1-NMR, MS-MS, LC-MS, GC-MS.	scientific
Leibniz-Institute of Arteriosclerosis Research	University of Münster	[phone]	48149	Münster	Domagkstr. 3	Germany	coronary heart disease, biomarkers, NMR, mass spectrometry	Research on biomarkers related to chd, stroke, Alzheimer disease risk. Specific interest in technologies: H1-NMR, MS-MS, LC-MS, GC-MS.	scientific
Department of Neurology	University of Regensburg	[phone]	93051	Regensburg	Universitätsstr. 84	Germany	alpha-synuclein, Parkinson, Neurodegeneration, synaptic plasticity	membrane microdomains and alpha-synuclein interaction in synaptic vesicle dynamics and plasticity	scientific
Institute for Clinical Chemistry	University of Regensburg	++49-941-944-6201	93053	Regensburg	Franz-Josef-Strauss-Allee 11	Germany	ABC Transporter, Lipid Biomarker, Biobanking	The research of the institute is directed towards the molecular analysis of metabolic and degenerative diseases. The central role of lipids in the pathogenesis of these disorders is a the main focus of the Institute of Clinical Chemistry. Our strategy combines basic research with clinical association studies enabling a rapid transfer of newly identified candidate markers from basic science to clinical diagnostics in order to support prediction, prevention, and therapy of diseases. The clinical part involves the Regensburg Diabetes Endpoint Prediction and Prevention Study (REDEPPS), an interdisciplinary platform that enables the establishment of large sample and data banks from patients with different endpoints or co-morbidities of diabetes, such as coronary heart disease, stroke, hypertension, obesity, nephropathy, retinopathy and neuropathy. Candidate markers which are verified for their diagnostic applicability in these cohorts are derived from basic research project focusing on the regulation of lipid homeostasis and vesicular traffic as related to disease pathogenesis. In this context the group has recently identified the ATP binding cassette transporter ABCA1 as a major regulator of HDL metabolism. The function of ABCA1 in the formation of lipid microdomains and vesicular transport is subject of a current project within the Transregional Collaborative Research Centre 6031 entitled Membrane Microdomains and Their Role in Human Disease funded by the DFG. The available technologies include high throughput genotyping platforms and DNA-arrays for genetic analysis, protein analysis by MALDI-TOF and the 2D-Gel Typhoon system, multicolour flow cytometry and fluorescence imaging as well as tandem mass spectrometry and NMR spectroscopy for lipids analysis. The methodological expertise of the institute is complemented by the competence centre for fluorescent bioanalysis (KFB) a public private partnership involving various institutes of the University of Regensburg.	scientific
Department of Physiological Chemistry	University of Veterinary Medicine Hannover	++49-511-953 8780	D-30559	Hannover	Bünteweg 17	Germany	Lipid microdomains, early detergent-resistant membranes, polarized protein sorting, colitis	Membrane transport, polarized protein sorting: role of lipid microdomains/detergent-resistant membranes, Intestinal membrane topology in IBD	scientific
Laboratory of Lipid Analysis	University of Ioannina	[phone]	451 10	Ioannina	Panepistemioupolis	Greece	Lung surfactant, apoptosis, inflammation	Analysis of lung surfactant lipids and their interaction with surfactant proteins Lipids as substrates for phospholipases: metabolism, structural studies, modeling Lipid modifications Lipids and signal transduction Techniques in Lipid Analysis: TLC, gas chromatography, HPLC (normal and reversed phase), Mass spectrometry (FAB, EI), Light scattering	scientific
Laboratory of Nutritional Bioactivation and Bioanalysis	Department of Biochemistry and Molecular Biology, University of Debrecen	[phone]	4012	Debrecen	Nagyerdei Krt. 98	Hungary	HPLC MS-MS, retinoic acid, carotenoid, PUFA metabolite	HPLC MS-MS of retinoids, carotenoids and PUFA metabolites (HETEs, HODEs, eicosanoids).	scientific
Laboratory of Molecular Stress Biology	Institute of Biochemistry, Biological Research Centre, HUng. Acad. Sci.	[phone]	H-6726	Szeged	Temesvari krt. 62.	Hungary	stress sensing, lipid molecular species, mass spectrometry, rafts	Regulation of heat shock gene expression by the physical state, lipid composition, mass spectrometry of lipids, microdomain organization of membranes in different systems, the mechanism of thermoadaptation, stress sensing and signaling, cellular thermosensors, etc.	scientific
Dept of Human Morphology and Developmental Biology	Semmelweis University	36 1 215 6924/3610	1094	Budapest	Tuzolto u. 58	Hungary	caveoae, lipid rafts	In my lab we are very much interested in caveolae, mainly the function of these special lipid raft	scientific
Dept. Biophysics and Cell Biology	University of Debrecen	++36-52-455866	4010	Debrecen	Egyetem ter 1.	Hungary	P-glycoprotein, lipid rafts, cytoskeletal anchorage	We wish to understand the relationships between catalytic activity, conformational changes and topological constraints of the ABC transporter P-glycoprotein (Pgp), both in the context of multidrug resistance (mdr) and its physiological roles, also to screen more efficient/ selective Pgp modulators. We are currently focusing on the localization of Pgp within and outside of lipid rafts, in conjunction with its conformational and catalytic characteristics. Our experimental systems include: (A) a novel flow-cytometric method to detect Pgp conformational changes upon its interactions with modulators, based on antibody competition, (B) a flow-cytometric platform for the measurement of raft-, and cytoskeleton-association of cell-surface proteins, applicable for rapid, simple, serial analysis of intermolecular associations between different cell surface proteins, (C) a simple method to completely block Pgp function using an anti-Pgp mAb, (D) a xenotransplantation system in SCID mice to study, by SPECT and PET, the effect of modulators, (E) methods for fluorescence resonance energy transfer (FRET) measurements on cell surface proteins to study inter-, and intramolecular distances. We have flow-cytometers, confocal microscope equipped with FCS, expertise in fluorescence-related methods, with special emphasis on FRET.	scientific
Sackler School of Medicine-Minerva Center	Tel Aviv University	972-3-6407842	69978	Tel Aviv	Levanon	Israel	fatty acid desaturases, nutrition,metabolic syndrome	Our interest is to understand the role of fatty acid metabolism : synthesis and desaturation specifically, in pathologies leading to the alteration of glucose and lipid homeostasis.The main emphasis is on nutritional essential fatty acids. We have developed animal and cell culture models of : hypercholesterolemia ,fatty liver and diabetes. We are employing "in vitro" and "in vivo" labelled precursors to follow up lipid synthesis, fatty acid desaturation, cholesterol , bile acid synthesis and lipid composition.We measure the enzymatic pathways involved. We utilize GC, HPLC and TLC methodologies , as well as biochemical and molecular biology determinations. Our purpose is to contribute to the understanding of the impact of nutritional fatty acids on the amelioration of lipid related diseases .	scientific
Dept of Biological Chemistry	Weizmann Institute	++972-8-9342704	76100	Rehovot	1 Herzl Street	Israel	sphingolipids, ceramide, glycolipids	For 15 years. my lab has worked on sphingolipids. We focus on sphingolipid synthesis, and also defective degradation in lysosomal storage diseases. More details can be found at: http://www.weizmann.ac.il/Biological_Chemistry/scientist/futerman/	scientific
ISOF/BioFreeRadicals	Consiglio Nazionale delle Ricerche	++39-051-639-8309	40129	Bologna	Via Piero Gobetti 101	Italy	trans lipid, radical stress, isomerization	Different aspects of the cis-trans isomerization of fatty acid residues by free radicals are developed: (a) Biomimetic studies using liposomes as a model for cell membranes and correlating the isomerization process with possible mechanisms of lipid alteration occurring in vivo. The role of different vitamins. (b) A general approach has been settled for building-up of trans lipid library with application to lipidomic researches. Analytical data on trans lipids and characterization of these lipid components in living organisms, thus contributing to the lipidomic target. This is also related to the examination of biological samples and correlation between trans lipid and radical stress. Endogenous formation of trans lipids and correlation with quality of life and various pathologies.	scientific
EPR Lipidomics	Dept Biochemistry and Molecular Biology	++39 080 5443367	70126	BARI	V. E. Orabona, 4	Italy	phospholipid bilayer, spin labeling EPR, lipoperoxidation, oxidative stress	Phospholipid bilayer structure and biochemical/biophysical properties. Spin labeling of phospholipids. EPR studies of spin labelled natural and artificial membranes (mitochondria, erythrocyte ghost, lipid vesicles, oriented planar membranes). Lipoperoxidation. Synthesis and purification of oxidized phospholipid species. EPR studies of the effects of oxidized phospholipids in model membranes and in membranes reconstituted with lipidomes from pathological tissues (animal models and humans) and from cultured pathological cell mutants. Mass spectrometry lipidomics of peroxidized phospholipids, mainly lecithins (in collaboration with the local university Chemistry Department).	scientific
Istituto Superiore di Sanità	Dept of Cell Biology and Neurosciences	++39-06-49903087	00161	ROME	viale Regina Elena, 299	Italy	isoprostanes, prostaglandins, oxidative stress	isoprostanes, prostaglandins, oxidative stress	scientific
Anna Maria Vaccaro Unit of Physiopatology of genetic diseases	Istituto Superiore di Sanita	++39 06 4990 2416	00161	Roma	Viale Regina Elena 299	Italy	sphingolipidoses, saposins, glucosylceramidase, anionic phospholids+	We works on a group of genetic diseases, the sphingolipidoses, characterized by the lysosomal storage of sphingolipids due to the defective activities of sphingolipid hydrolases or of their physiological activators. In particular, we have investigated the alterations in glucosylceramide catabolism in Niemann Pick disease type C. We have shown that the storage of cholesterol in Niemann Pick type C modulates the level and subcellular localization of glucosylceramidase affecting in this way the glucosylceramide degradation (Salvioli et al., J.Biol. Chem. 279, 17674-17680, 2004). Moreover, we have shown that the glucosylceramidase N370S mutation, the most common mutation in Gaucher disease, affects the capacity of the enzyme to interact with anionic phospholipid-containing membranes and saposin C, the main physiological activators of glucosylceramidase (Salvioli et al., Biochem. J., 390, 95-105, 2005). Our work is also focused on the study of the mechanism/s of action of saposins and on their interaction with lipids, especially phospholipids. Actually we have contribute to define the mechanism of action of saposin C (Vaccaro et al.,J.Biol.Chem.,272, 16862-16867,1997, Salvioli et al., FEBS Letters, 472, 1721, 2000), of saposin D (Ciaffoni et al., J. Biol. Chem., 276, 31583-31589, 2001) and of saposin B (Ciaffoni et al., J. Lip. Res., 47, 1045-1053, 2006).	scientific
Department of Cell Biology and Oncology	Mario Negri Sud Institute	[phone]	66030	Santa Maria Imbaro	Via Nazionale 8/A	Italy	phosphoinositides, glycerophosphoinositols, phospholipases, LXR-FXR nuclear receptors	Phosphoinositide metabolism and related diseases. Our expertises include: -- phosphoinositide analysis by standard HPLC, TLC, Lipid binding domain recognition -- PI kinase and phosphatase assays -- inositol phospate and glycerophosphoinositol analysis by HPLC and MS -- metabolism of the phosphoinositides and their metabolites -- Morphological approaches with reference to the LBD (IF, EM, Tomography, FRET, FRAP, Correlative light/EM) Our aim is to set and apply the lipidomic approach to the lipid profiling of cell models of different pathological conditions (Overexpressing or KO for proteins/enzymes involved in lipid metabolism and related diseases) Ongoing projects: The glycerophosphoinositols (GPIs) as modulators of the Rho-family cycle: Our studies have indicated that the GPIs, the cellular derivatives of the phosphoinositides, are modulators of actin cytoskeleton assembly through an action on the Rho GTPases (Corda et al., 2002, Mancini et al., 2003). These compounds have thus become the focus of further studies aimed at elucidating their mechanism of action (Mariggiò et al, 2006) and at exploiting them as potential leads for drug development in actin-related diseases (Wiscott-Aldrich syndrome, X-linked mental retardation due to defect in PIX, common variable immunodeficiency). Diseases due to defects in the PI phosphatases: OCRL-1, MTM1 and MTMR2 (Lowe Syndrome, Myotubular Myopathy, and Type 4B Charcot-Marie-Tooth). The aim of our project is to identify and validate the enzymes controlling the PI cycle as pharmacological targets, the activities of which can be modulated by inhibitors and/or activators to re-establish the correct balance between the PIs pools in the above-mentioned conditions in which this balance has been compromised by the lack or deregulation of the PIPtases. A similar approach is currently being undertaken in the case of the deregulation of PI 3-kinase (PI3K) pathway, which occurs in cancer, thrombotic diseases, inflammation and diabetes. Other projects involving aspects of the lipid metabolism are: -role of PLA2 in membrane traffic -mechanism of membrane fissioning mediated by the protein BARS -Regulation of lipid metabolism by LXR and FXR nuclear receptor and relevance in the development of colon cancer	scientific
Department of Cell Biology and Oncology	Mario Negri Sud Institute	[phone]	66030	Santa Maria Imbaro	Via Nazionale 8/A	Italy	phosphoinositides, glycerophosphoinositols, phospholipases, LXR-FXR nuclear receptors	Phosphoinositide metabolism and related diseases. Our expertises include: -- phosphoinositide analysis by standard HPLC, TLC, Lipid binding domain recognition -- PI kinase and phosphatase assays -- inositol phospate and glycerophosphoinositol analysis by HPLC and MS -- metabolism of the phosphoinositides and their metabolites -- Morphological approaches with reference to the LBD (IF, EM, Tomography, FRET, FRAP, Correlative light/EM) Our aim is to set and apply the lipidomic approach to the lipid profiling of cell models of different pathological conditions (Overexpressing or KO for proteins/enzymes involved in lipid metabolism and related diseases) Ongoing projects: The glycerophosphoinositols (GPIs) as modulators of the Rho-family cycle: Our studies have indicated that the GPIs, the cellular derivatives of the phosphoinositides, are modulators of actin cytoskeleton assembly through an action on the Rho GTPases (Corda et al., 2002, Mancini et al., 2003). These compounds have thus become the focus of further studies aimed at elucidating their mechanism of action (Mariggiò et al, 2006) and at exploiting them as potential leads for drug development in actin-related diseases (Wiscott-Aldrich syndrome, X-linked mental retardation due to defect in PIX, common variable immunodeficiency). Diseases due to defects in the PI phosphatases: OCRL-1, MTM1 and MTMR2 (Lowe Syndrome, Myotubular Myopathy, and Type 4B Charcot-Marie-Tooth). The aim of our project is to identify and validate the enzymes controlling the PI cycle as pharmacological targets, the activities of which can be modulated by inhibitors and/or activators to re-establish the correct balance between the PIs pools in the above-mentioned conditions in which this balance has been compromised by the lack or deregulation of the PIPtases. A similar approach is currently being undertaken in the case of the deregulation of PI 3-kinase (PI3K) pathway, which occurs in cancer, thrombotic diseases, inflammation and diabetes. Other projects involving aspects of the lipid metabolism are: -role of PLA2 in membrane traffic -mechanism of membrane fissioning mediated by the protein BARS -Regulation of lipid metabolism by LXR and FXR nuclear receptor and relevance in the development of colon cancer	scientific
Biochemistry group	University Milano-Bicocca	++39-02-6448-8203	20052	Monza	Via Cadore, 48	Italy	Alzheimer, Lipid rafts, signal transduction	Lipids and neurodegenerative diseases Lipid rafts and disease Lipid rafts and signal transduction Lipid-protein interaction	scientific
Department of Experimental Medicine	University Milano-Bicocca	++39-02-6448-8203	20052	Monza	Via Cadore, 48	Italy	Alzheimer, Lipid rafts, signal transduction	Lipids and neurodegenerative diseases Lipid-protein interaction Lipid rafts and signal transduction Lipid rafts and disease	scientific
Dipartimento di Scienze Biochimiche	University of Florence	++390554598328	50143	Firenze	Viale G.B. Morgagni 50	Italy	sphingosine 1-phosphate, lipid microdomain, ceramide, ceramidase	Sphingosine 1-phosphate and its mechanism of action. Regulation of cellular sphingosine 1-phosphate content. Functional role of membrane lipid microdomains in sphingosine 1-phosphate production and signalling.	scientific
Department of Clinical and Experimental Medicine, Physiopathology, University School of Medicine	University of Perugia	++39-075-5729085	06100	Perugia	Policlinico Monteluce, via Brunamonti	Italy	intranuclear lipid, DNA-lipid, RNA-lipid	We have demonstrated that a phospholipid cellular fraction is associated with chromatin. The presence, described by histochemical and biochemical techniques, is not due to membrane contaminations. The chromatin phospholipid fraction (CPF) can be considered a chromatin minor component. The CPF differ from that of microsomes and nuclear membranes as regard composition and turnover and changes in relation to hepatocyte maturation and proliferation. The main modifications, evident in several cellular functions, regard principally phosphatidylcholine (PC) and sphingomyelin (SM) that are metabolised directly in the nuclei in a very short time with a fine modulation of the enzimatic activities, regulated by PLs. The presence of a complex of base exchange enzymes for PC synthesis, a neutral sphingomyelinase (N-SMase), a sphingomyelin-synthase (SM-synthase), a phosphatidylcholine-dependent phospholipase C (PC-PLC) and a sphingomyelin-synthase-reverse (SM-synthase-reverse), which synthesises PC from SM have been demonstrated. These enzymes differ for pH and Km optima from those present in nuclear membranes, suggesting the possible existence of different isoforms. These results strongly support the presence of a metabolic machinery in the chromatin which probably is regulated in relation to cellular function. The PC-PLC and SM-synthase enrich the intranuclear pool of diacylglicerol (DAG), whereas the N-SMase and SM-synthase-reverse enrich the intranuclear pool of ceramide. DAG and ceramide are know as second messengers which play an important role in different physiological conditions, acting as a stimulating (DAG) and inhibitory factor (ceramide) of protein kinase C (PKC) The hypothesis has been made that the lipid messenger can favour the nuclear molecular events which precede DNA synthesis in liver regeneration. The results show that the DAG pool increases at the beginning of S phase, whereas the ceramide pool later increases. In the erythroleukemic cells, treated with DMSO and/or D3 vitamin, the N-SMase activity increases during the apoptotic process, whereas PC-PLC activity increases during cell differentiation. It will be interesting to know if DAG and ceramide, produced directly in chromatin, can play a role in cellular functions. 1. Viola Magni M.P., Gahan P.B., Albi E., Iapoce R. and Gentilucci P.F. "Chromatin phospholipids and DNA synthesis in hepatic cells". Bas. Appl. Histochem. 29, 253-259, 1985. I.F. 1.041 2. .Viola Magni M.P., Gahan P.B., Albi E., Iapoce R. and Gentilucci P.F. "Phospholipids in chromatin: incorporation of 32PO24 in different subcellular fraction of hepatocytes" Cell Biochem. and Function 4, 283-288, 1986. I.F. 1.452 3. Viola Magni M.P., Gahan P.B., Albi E., Iapoce R. and Gentilucci P.F. "Synthesis of chromatin phospholipids" Bas. Appl. Histochem. 31, 355-364, 1987. I.F. 1.041 4. Albi E., Viola Magni M.P. and Gahan P.B. "Age-related changes in chromatin phospholipid fraction" The liver, metabolism and ageing, Eurage 13,189-188, 1989. 5. Gahan P.B., Albi E. and Viola Magni M.P. "Changes with age in phospholipid composition of rat liver cell nuclei and nuclear envelopes". Drug metabolism, liver injury and ageing, Eurage, 16, 229-236, 1991. 6. Albi E., Viola Magni M.P., Lazzarini R. and Gahan P.B. "Chromatin phospholipid changes during rat liver development" Cell Biochem. and Function 9, 119-123, 1991. 1.452. I.F. 1.452 7. Albi E. "Presenza e ruolo dei fosfolipidi nella cromatina" Tesi di Dottorato in Patologia Cellulare e Molecolare. 1991. 8. Fraschini A., Albi E., Gahan P.B. and Viola Magni M.P. "Tem cytochemical study of the localization of phospholipids in interphase chromatin in rat hepatocytes" Histochemistry 97, 225-235, 1992. I.F 9. Albi E. , Mersel M., Leray C., Tomassoni M.L. and Viola-Magni M.P. Rat Liver Chromatin Phospholipids" Lipids 29, 715-719- 1994. I.F. 2.164 10. Albi E., Micheli M., Viola-Magni M.P. "Phospholipids and nuclear RNA" Cell Biol. Intern. 20, 6, 1996. I.F. 1.092 11. Albi E., Viola-Magni M.P. "Choline base exchange activity in rat hepatocyte nuclei and nuclear membrane" Cell Biol.Intern 21, 217-221, 1997. I.F. 1.092 12. Albi E., Tomassoni M.L., Viola-Magni M.P. "Effect of lipid composition on rat liver nuclear membrane fluidity" Cell Biochem. and Funct, 15, 181-190, 1997. I.F. 1.452 13. Albi, E. and Viola Magni, M.P. "Chromatin neutral spingomyelinase and its role in hepatic regeneration. Biochim. Biophys. Res. Commun., 236, 29-33, 1997. I.F. 2.836 14. Micheli,M., Albi,E, Leray,C., and Viola Magni,M.P. "Nuclear sphingomyelin protects RNA from RNase action" FEBS Letters 431, 443-447, 1998. I.F. 3.609 15. Tomassoni,M.L., Albi,E., and Viola Magni,M.P. Changes of nuclear membrane fluidity during rat liver regeneration Biochem. Mol. Biol. Intern. 47, 1049-1059, 1999. 16. Albi,E., Peloso,I., and Viola Magni,M.P. Nuclear Membrane Sphingomyelin-Cholesterol Changes in Rat Liver after Hepatectomy Biochem. Biophys. Res. Commun. 262, 692-695, 1999. I.F. 2.836 17. Albi,E., Viola Magni,M.P. Sphingomyelin-Synthase in Rat Liver Nuclear Membrane and Chromatin FEBS Letters 460, 369-372, 1999. I.F. 3.609 18. Albi,E., and Viola Magni,M.P. Phosphatidylcholine-Dependent Phospholipase C in Rat Liver Chromatin Biochem.Biophys.Res.Commun. 265, 640-643, 1999. I.F. 2.836 19. Albi,E., Viola Magni,M.P. The presence and the role of chromatin cholesterol in rat liver regeneration Journal of Hepatology 36, 395-400, 2002. I.F. 5.285 20. Caso,V., Panarelli,P., Albi,E., Viola-Magni,M.P., Parnetti,L., Gallai,V. Phospholipid autoantibodies: time for a new immuno-assay? Clin Exp Hypertens. 24: 511-516, 2002. I.F. 0.816 21. Panarelli,P., Viola-Magni,M.P., and Albi E. Antiphosphatidylinositol antibody in deep venous thrombosis patients Int.J. Immunopath. Pharm. 2003, 16: 61-6. I.F. 3.927 22. Albi,E., Cataldi,S., Rossi,G., and Viola Magni,M.P. A possible role of cholesterol-sphingomyelin/phosphatidylcholine in nuclear matrix during rat liver regeneration. J. Hepatology 2003, 38: 623-8. I.F. 5.283 23. Albi,E., Pieroni,S., Viola Magni,M.P., and Sartori,C Chromatin sphingomyelin changes in cell proliferation and/or apoptosis induced by ciprofibrate. J. Cell Physiol. 2003, 196:354-61. I.F. 5.436 24. Valeria Caso, Lucilla Parnetti, Paolo Panarelli, Maria Pia Viola Magni, MD, Virgilio Gallai, Elisabetta Albi Selection of Thrombogenetic Antiphospholipid Antibodies In Cerebrovascular Disease Patients J.Neurol 2003, 250: 593-597. I.F. 2.778 25. Albi,E., Rossi,G., Maraldi,N.M., Viola Magni,M.P., Cataldi,S., Solimando, L., Zini,N. Involvement of nuclear Phosphatidylinositol-dependent Phospholipases C in cell cycle progression during rat liver J. Cell Physiol. 2003, 197: 181-188. I.F. 5.463 26. Elisabetta Albi, Remo Lazzarini and Mariapia Viola Magni Reverse Sphingomyelin-Synthase in Rat Liver Chromatin FEBS Letters 2003, 549(1-3):152-156. I.F. 3.609 27. Elisabetta Albi, Samuela Cataldi, Mariapia Viola Magni and Claudia Sartori Plasmalogens in rat liver chromatin: new molecules involved in cell proliferation J. Cell Physiol, 2004, 201(3):439-46. I.F. 5.463 28. Elisabetta Albi, Samuela Cataldi, Elisa Bartoccini, Mariapia Viola Magni, Francesca Marini, Francesca Mazzoni, Giuseppe Rainaldi, Monica Evangelisti, Mercedes Garcia-Gil Nuclear sphingomyelin pathway in serum deprivation-induced apoptosis of embryonic hippocampal cells J Cell Physiol, 2005. I.F. 5.463 29. Elisabetta Albi, Caterina AM La Porta , Samuela Cataldi, Mariapia Viola Magni Nuclear sphingomyelin-synthase and protein kinase C delta in melanoma cells Arch. Biochem Biophys, 2005, 438:156-61. I.F. 2.338	scientific
Department of Clinical and Experimental Medicine, Physiopathology, University School of Medicine	University of Perugia	++39-075-5729085	06100	Perugia	Policlinico Monteluce, via Brunamonti	Italy	intranuclear lipid, DNA-lipid, RNA-lipid	We have demonstrated that a phospholipid cellular fraction is associated with chromatin. The presence, described by histochemical and biochemical techniques, is not due to membrane contaminations. The chromatin phospholipid fraction (CPF) can be considered a chromatin minor component. The CPF differ from that of microsomes and nuclear membranes as regard composition and turnover and changes in relation to hepatocyte maturation and proliferation. The main modifications, evident in several cellular functions, regard principally phosphatidylcholine (PC) and sphingomyelin (SM) that are metabolised directly in the nuclei in a very short time with a fine modulation of the enzimatic activities, regulated by PLs. The presence of a complex of base exchange enzymes for PC synthesis, a neutral sphingomyelinase (N-SMase), a sphingomyelin-synthase (SM-synthase), a phosphatidylcholine-dependent phospholipase C (PC-PLC) and a sphingomyelin-synthase-reverse (SM-synthase-reverse), which synthesises PC from SM have been demonstrated. These enzymes differ for pH and Km optima from those present in nuclear membranes, suggesting the possible existence of different isoforms. These results strongly support the presence of a metabolic machinery in the chromatin which probably is regulated in relation to cellular function. The PC-PLC and SM-synthase enrich the intranuclear pool of diacylglicerol (DAG), whereas the N-SMase and SM-synthase-reverse enrich the intranuclear pool of ceramide. DAG and ceramide are know as second messengers which play an important role in different physiological conditions, acting as a stimulating (DAG) and inhibitory factor (ceramide) of protein kinase C (PKC) The hypothesis has been made that the lipid messenger can favour the nuclear molecular events which precede DNA synthesis in liver regeneration. The results show that the DAG pool increases at the beginning of S phase, whereas the ceramide pool later increases. In the erythroleukemic cells, treated with DMSO and/or D3 vitamin, the N-SMase activity increases during the apoptotic process, whereas PC-PLC activity increases during cell differentiation. It will be interesting to know if DAG and ceramide, produced directly in chromatin, can play a role in cellular functions. 1. Viola Magni M.P., Gahan P.B., Albi E., Iapoce R. and Gentilucci P.F. "Chromatin phospholipids and DNA synthesis in hepatic cells". Bas. Appl. Histochem. 29, 253-259, 1985. I.F. 1.041 2. .Viola Magni M.P., Gahan P.B., Albi E., Iapoce R. and Gentilucci P.F. "Phospholipids in chromatin: incorporation of 32PO24 in different subcellular fraction of hepatocytes" Cell Biochem. and Function 4, 283-288, 1986. I.F. 1.452 3. Viola Magni M.P., Gahan P.B., Albi E., Iapoce R. and Gentilucci P.F. "Synthesis of chromatin phospholipids" Bas. Appl. Histochem. 31, 355-364, 1987. I.F. 1.041 4. Albi E., Viola Magni M.P. and Gahan P.B. "Age-related changes in chromatin phospholipid fraction" The liver, metabolism and ageing, Eurage 13,189-188, 1989. 5. Gahan P.B., Albi E. and Viola Magni M.P. "Changes with age in phospholipid composition of rat liver cell nuclei and nuclear envelopes". Drug metabolism, liver injury and ageing, Eurage, 16, 229-236, 1991. 6. Albi E., Viola Magni M.P., Lazzarini R. and Gahan P.B. "Chromatin phospholipid changes during rat liver development" Cell Biochem. and Function 9, 119-123, 1991. 1.452. I.F. 1.452 7. Albi E. "Presenza e ruolo dei fosfolipidi nella cromatina" Tesi di Dottorato in Patologia Cellulare e Molecolare. 1991. 8. Fraschini A., Albi E., Gahan P.B. and Viola Magni M.P. "Tem cytochemical study of the localization of phospholipids in interphase chromatin in rat hepatocytes" Histochemistry 97, 225-235, 1992. I.F 9. Albi E. , Mersel M., Leray C., Tomassoni M.L. and Viola-Magni M.P. Rat Liver Chromatin Phospholipids" Lipids 29, 715-719- 1994. I.F. 2.164 10. Albi E., Micheli M., Viola-Magni M.P. "Phospholipids and nuclear RNA" Cell Biol. Intern. 20, 6, 1996. I.F. 1.092 11. Albi E., Viola-Magni M.P. "Choline base exchange activity in rat hepatocyte nuclei and nuclear membrane" Cell Biol.Intern 21, 217-221, 1997. I.F. 1.092 12. Albi E., Tomassoni M.L., Viola-Magni M.P. "Effect of lipid composition on rat liver nuclear membrane fluidity" Cell Biochem. and Funct, 15, 181-190, 1997. I.F. 1.452 13. Albi, E. and Viola Magni, M.P. "Chromatin neutral spingomyelinase and its role in hepatic regeneration. Biochim. Biophys. Res. Commun., 236, 29-33, 1997. I.F. 2.836 14. Micheli,M., Albi,E, Leray,C., and Viola Magni,M.P. "Nuclear sphingomyelin protects RNA from RNase action" FEBS Letters 431, 443-447, 1998. I.F. 3.609 15. Tomassoni,M.L., Albi,E., and Viola Magni,M.P. Changes of nuclear membrane fluidity during rat liver regeneration Biochem. Mol. Biol. Intern. 47, 1049-1059, 1999. 16. Albi,E., Peloso,I., and Viola Magni,M.P. Nuclear Membrane Sphingomyelin-Cholesterol Changes in Rat Liver after Hepatectomy Biochem. Biophys. Res. Commun. 262, 692-695, 1999. I.F. 2.836 17. Albi,E., Viola Magni,M.P. Sphingomyelin-Synthase in Rat Liver Nuclear Membrane and Chromatin FEBS Letters 460, 369-372, 1999. I.F. 3.609 18. Albi,E., and Viola Magni,M.P. Phosphatidylcholine-Dependent Phospholipase C in Rat Liver Chromatin Biochem.Biophys.Res.Commun. 265, 640-643, 1999. I.F. 2.836 19. Albi,E., Viola Magni,M.P. The presence and the role of chromatin cholesterol in rat liver regeneration Journal of Hepatology 36, 395-400, 2002. I.F. 5.285 20. Caso,V., Panarelli,P., Albi,E., Viola-Magni,M.P., Parnetti,L., Gallai,V. Phospholipid autoantibodies: time for a new immuno-assay? Clin Exp Hypertens. 24: 511-516, 2002. I.F. 0.816 21. Panarelli,P., Viola-Magni,M.P., and Albi E. Antiphosphatidylinositol antibody in deep venous thrombosis patients Int.J. Immunopath. Pharm. 2003, 16: 61-6. I.F. 3.927 22. Albi,E., Cataldi,S., Rossi,G., and Viola Magni,M.P. A possible role of cholesterol-sphingomyelin/phosphatidylcholine in nuclear matrix during rat liver regeneration. J. Hepatology 2003, 38: 623-8. I.F. 5.283 23. Albi,E., Pieroni,S., Viola Magni,M.P., and Sartori,C Chromatin sphingomyelin changes in cell proliferation and/or apoptosis induced by ciprofibrate. J. Cell Physiol. 2003, 196:354-61. I.F. 5.436 24. Valeria Caso, Lucilla Parnetti, Paolo Panarelli, Maria Pia Viola Magni, MD, Virgilio Gallai, Elisabetta Albi Selection of Thrombogenetic Antiphospholipid Antibodies In Cerebrovascular Disease Patients J.Neurol 2003, 250: 593-597. I.F. 2.778 25. Albi,E., Rossi,G., Maraldi,N.M., Viola Magni,M.P., Cataldi,S., Solimando, L., Zini,N. Involvement of nuclear Phosphatidylinositol-dependent Phospholipases C in cell cycle progression during rat liver J. Cell Physiol. 2003, 197: 181-188. I.F. 5.463 26. Elisabetta Albi, Remo Lazzarini and Mariapia Viola Magni Reverse Sphingomyelin-Synthase in Rat Liver Chromatin FEBS Letters 2003, 549(1-3):152-156. I.F. 3.609 27. Elisabetta Albi, Samuela Cataldi, Mariapia Viola Magni and Claudia Sartori Plasmalogens in rat liver chromatin: new molecules involved in cell proliferation J. Cell Physiol, 2004, 201(3):439-46. I.F. 5.463 28. Elisabetta Albi, Samuela Cataldi, Elisa Bartoccini, Mariapia Viola Magni, Francesca Marini, Francesca Mazzoni, Giuseppe Rainaldi, Monica Evangelisti, Mercedes Garcia-Gil Nuclear sphingomyelin pathway in serum deprivation-induced apoptosis of embryonic hippocampal cells J Cell Physiol, 2005. I.F. 5.463 29. Elisabetta Albi, Caterina AM La Porta , Samuela Cataldi, Mariapia Viola Magni Nuclear sphingomyelin-synthase and protein kinase C delta in melanoma cells Arch. Biochem Biophys, 2005, 438:156-61. I.F. 2.338	scientific
Department of Internal Medicine	University of Perugia	0039 75 5857423	06122	Perugia	Via del Giochetto	Italy	pollen phospholipids, brain mitochondria, anionic phospholipids, cardiolipin	I)Plant pollens are an important source of environmental antigens that stimulate allergic responses. In addition to acting as vehicled for foreign protein antigens, they contain different molecular species of lipids, which are necessary in the reproduction of higher plants. The CD1 family of nonpolymorphic major histocompatibility complex-related molecules is highly conserved in mammals, and has been shown to present microbial and self lipids to T cells. We provided evidence that pollen lipids may be recognized as antigens by human T cell through a CD1-dependent pathway. Amonng phospholipids extracted from cypress grains, phosphatidylcholine (PC)and phosphatidylethanolamine (PE)were able to stimulate the proliferation of T cells from cypresse-sensitive subjects. Particularly, 18:2/18:2 PC, a predominat component of cypress PC, could be one of the specific targets in pollen grain capture and recognition by CD1. Also pollen-derived unsaturated PE are antigens recognized by a variety of T clones derived from allergic subjects. The nature of the acyl chains in the lipid antigens contributes enormously to immunogenicity.(Agea et al. Journal Exp. Med. (2005) 202:295-308. The nature of lipid-protein interaction between phospholipids and CD1 is a target for future investigation. The antigenic properties of glycolipids extracted and purified from pollens will be also evaluated. II) We are also involved in projects aimed at studying the role of anionic phospholipids, particularly cardiolipin, in the functionality of mitochondria in the nervous tissue. A major finding of our previous studies was the effect exerted by exogenous phospholipids on the release of cytochrome c from the inner mitochondrial membrane and on the transmembrane potential of brain mitochondria. Mitochondria were enriched with exogenous phosoholipids through a fusion process in the outer mitochondrial membrane. The experimental system utilized a fluorescent probe inserted in the outer mitochondrial membrane to follow the kinetics of incorporation of lipids in mitochondria. By using a selective probe for cardiolipin, it has been demonstrated thet the fused lipid can reach the inner mitochondrial membrane and influence the membrane potential. We found that exogenous cardiolipin reinforced the binding of cytochrome c with the inner mitochondria, thus hindering its release following different stimuli. The interaction of cytochrome c with the components of the inner mitochondrial membrane is object of investigation. The mode of membrane association of cytochrome c is studied by using a model system of cytochrome c reconstituted in different cardiolipins. (Piccotti et al. JBC 2002, 277:12075-12081, Piccotti et al. J. Membr. Biol. 2004, 198:43-53)	scientific
Department of Internal Medicine, Section of Biochemistry	University of Perugia	++39-075-585-7420	I-06122	Perugia	Via del Giochetto	Italy	phospholipase A2, lipid mediators, PAF	This group is interested in the identification and localization of various phospholipases A2 in intracellular compartments. Particular attention is devoted to secretory PLA2s with the aim of uderstanding their functions in normal and pathological conditions. We have already demonstrated the presence of a group IIA sPLA2 from cerebral cortex mitochondria and its release under energy-deficient conditions and the location of group V sPLA2 in the nuclei of cultured PC-12 and U251 astrocytome cells of group V sPLA2 (Macchioni et al., J Biol Chem. (2004) Vol. 279, 36 (3), 37860-37869). Furthermore we have set up a procedure for a continuous monitoring PLA2 activities in cultured cells in vivo or subcellular organelles. Since many cell types release low molecular weight sPLA2, we are also studying the routing for their secretion of their translocation to intracellular compartments. This group is also interested on the formation of lipid mediators and particularly of PAF. We have identified the presence of the enzymes for its synthesis in the nervous tissue.	scientific
Department of Internal Medicine, Section of Biochemistry	University of Perugia	++39-075-585-7420	I-06122	Perugia	Via del Giochetto	Italy	phospholipase A2, lipid mediators, PAF	This group is interested in the identification and localization of various phospholipases A2 in intracellular compartments. Particular attention is devoted to secretory PLA2s with the aim of uderstanding their functions in normal and pathological conditions. We have already demonstrated the presence of a group IIA sPLA2 from cerebral cortex mitochondria and its release under energy-deficient conditions and the location of group V sPLA2 in the nuclei of cultured PC-12 and U251 astrocytome cells of group V sPLA2 (Macchioni et al., J Biol Chem. (2004) Vol. 279, 36 (3), 37860-37869). Furthermore we have set up a procedure for a continuous monitoring PLA2 activities in cultured cells in vivo or subcellular organelles. Since many cell types release low molecular weight sPLA2, we are also studying the routing for their secretion of their translocation to intracellular compartments. This group is also interested on the formation of lipid mediators and particularly of PAF. We have identified the presence of the enzymes for its synthesis in the nervous tissue.	scientific
Funato Group	Hiroshima University	++81-82-424-7925	739-8528	Higashi-Hiroshima	1-4-4, Kagamiyama	Japan	ceramide, GPI, transport	lipid trafficking	scientific
Department of Biochemistry and Molecular Biology	Osaka University Graduate School of Medicine	++81-6-6879-3283	662-0018	Suita	2-2 Yamadaoka	Japan	HPLC/MS, sphingolipids, phospholipids	I has developed an HPLC/ESI/MS-based method capable of analyzing a wider range of lipid classes from neutral lipids to phospholipids on a single chromatographic run with three solvent gradients and post-column mixing of solvent that helps ESI of lipids eluted earlier with non-polar solvents. We used this method to study on lipid anomaly of a variety of gene-targeted mice tissues and subcellular organellae [e.g., Takagi, S., Tojo, H., Tomita, S., Sano, S., Itami, S., Hara, M., Inoue, S., Horie, K., Kondoh, G., Hosokawa, K., Gonzalez, F.J. and Takeda, J. Alteration of the 4-sphingenine scaffolds of ceramides in keratinocyte-specific Arnt deficient mice affects skin barrier function. J. Clin. Invest. 112, 1372-1382 (2003)]. We are now extending the method amenable to analyzing more polar lipids that cantain important bioactive lipids. We eventually would like to develop a very comprehensive lipid analyzing system.	scientific
Department of Metabolome, Graduate School of Medicine	The University ofTokyo	81-3-5841-3651	113-0033	Tokyo	7-3-1, Hongo, Bunkyo-ku	Japan	lipid, database	create lipid database "LipidBank"	scientific
Laboratory Genetic Metabolic Diseases	Academic Medical Center	[phone]	NL-1100 DD	Amsterdam	P.O. Box 22660	Netherlands	tandem-Mass Spectrometry, glycerophospholipids, cardiolipin, ceramides	HPLC-tandem mass spectrometric analysis of lipid compositions at different levels of complexity, such as cell compartments/organelles, cells, tissues in order to clarify/study biochemical processes in relation to disease based disorders. Research is biomedical orientated in a university hospital setting and findings are where possible translated into diagnostic tests.	scientific
DSM	analysis/spectrometry	0031 152792278	2600 MA	Delft	p.o. box 1	Netherlands	ceramides, sphingolipids	ceramides, sphingolipids	industrial
Lipid laboratory	Dept Internal Medicine, UMC Nijmegen	-	6500 HB	Nijmegen	Geert Grooteplein zuid 8	Netherlands	GC-MS, steroids, lipoproteins	lipidperoxydation and antioxidants, lipid transfer, steroids, nutrients	scientific
Physics of Life Processes	Leiden Institute of Physics, Leiden University	[phone]	2333 CA	Leiden	Niels Bohrweg 2	Netherlands	cell signaling, model systems, lipid rafts, single-molecule biophysics, fluorescence microscopy	cell signaling, model systems, lipid rafts, single-molecule biophysics, fluorescence microscopy	scientific
NIOZ	NIOZ	31 222 369582	1790 AB	Den Burg	P.O. Box 59	Netherlands	organic geochemistry, biomarker	As part of the ICOMM (http://icomm.mbl.edu/)work we are building up a lipid database. If possible, we want to combine our data with yours. Firstly, we are interested in a procedure to find data from the database. What programs are developed, how do they work. If there is no such program exists, one has to be created. We hope we can work with you to get things working.	scientific
Philips Research, Molecular Diagnostics	Philips	[phone]	5656 AE	Eindhoven	High Tech Campus 4	Netherlands	cholsterol, membrane microdomains, sphingolipids, oxidized lipids, lipoproteins	Bioactive lipids relevant to diseases	industrial
Bijvoet Center	Utrecht University	++31-30-2533498	NL-584 CH	Utrecht	Padualaan 8	Netherlands	membrane membrane-protein rafts	Lipids is membranes and as signalling entities	scientific
Institute of Biomembranes	Utrecht University	[phone]	3584 CH	Utrecht	Padualaan 8	Netherlands	lipid cell biology - lipid mass spectrometry - model membrane biochemistry	The Institute of Biomembranes is an interdisciplinary research institute and graduate school at Utrecht University, accommodating 18 research groups from the faculties of Science, Medicine, and Veterinary Medicine. The famous lipid biochemist Laurens van Deenen was one of the founding members of the institute in 1991, but unfortunately deceased in 1994. To remember him, since 2004 the Institute annually awards the "van Deenen Medal" to a leading active scientist in biomembrane research (http://ib.bio.uu.nl/). The present director chairs a specific support action of the European Commission entitled "The European Lipidomics Initiative" (www.lipidomics.net). A number of groups in the institute work on questions related to the structure and function of lipids in cells, blood (lipoproteins) and model membranes. Notably, a dedicated effort in lipid mass spectrometry is ongoing in the Dept. of Biochemistry and Cell Biology, Faculty of Veterinary Medicine (http://www.vet.uu.nl/bc).	scientific
Nutrition, metabolism and genomics group	Wageningen University	++31-317-485787	6703 HD	Wageningen	Bomenweg 2	Netherlands	transcriptomics, PPARs, fatty acids	Our group is interested in regulation of gene expression by fatty acids. We use transcriptomics (in house affymetrix platform) in combination with knock-out mice models to investigate the overall impact as well as the mechanism of fatty acid-dependent gene regulation in numerous organs (small intestine, liver, heart). Our expertise is: 1) design, analysis and interpretation of transcriptomics experiments, and 2) pathway mapping of transcriptomics data with special emphasis on lipid metabolism	scientific
Preclinical sciences	GE Healthcare	[phone]	0401	Oslo	Nycoveien 2	Norway	Phospholipids, blood, drug development	Phospholipid analysis related to contrast agents for medical imaging. Analyses of blood samples in order to describe pharmacokinetics/toxicokinetics.	industrial
Inst. for Cancer Research/Sandvigs group:Intracellular transport	The Norwegian Radium Hospital	47 22934294	0310	Oslo	Montebello	Norway	glycosphingolipids, toxins, cholesterol, rafts	Studies of the role of different lipids on intracellular transport of protein toxins. For recent publications, please see the home pages:http://radium.no/sandvig/ We have been studying the role of cholesterol in transport, and we have been investigating cells with mutations in (glyco)sphingolipid synthesis. Studies on the roles of glycospingolipids are in progress.	scientific
Laboratory of Transcriptional Regulation	Centre for Medical Biology PAS	++48-42-2723639	93-232	Lodz	Lodowa 106	Poland	ABC transporters, gene expression, transcription factors	Lipid hormones and second messengers - their direct and indirect effects on gene expression. Lipid-binding transcription factors. Lipid transporters from the ABC superfamily - mechanism of action, regulation of expression.	scientific
Department of Animal Physiology and Biophysics	Faculty of Biology, University of Bucharest	00-40-21-318 15 69	050095	Bucharest	Splaiul Independentei, 91-95	Romania	Plane lipid bilayers, Liposomes, Pharmacology	Our group is interested to test antidepressants (natural and synthetic) and neuroleptics on plane lipid bilayers by electrical recordings and liposomes by fluorescence polarisation.	scientific
Institute of Biophysics, Faculty of Medicine	University of Ljubljana	-386 1 5437600	1000	Ljubljana	Lipiceva 2	Slovenia	vesicle shapes, membrane trafficking, amphitropic proteins	Mechanical properties of lipid membranes. Theoretical and experimental studies of phospholipid vesicle shape behavior. Shape induced mechanisms for the lateral segregation of membrane components. Role of vesicle shapes in intracellular membrane trafficking. Mechanism of action of amphitropic proteins studied by vesicle shape transformations induced by their binding to vesicle membranes.	scientific
INSTITUTO DE PARASITOLOGIA Y BIOMEDICINA LOPEZ-NEYRA, Working Group: BIOCHEMISTRY AND MOLECULAR PHARMACOLOGY. Dr. FRANCISCO GAMARRO	CONSEJO SUPERIOR DE INVESTIGACIONES CIENTÍFICAS	0034958181667	18100	ARMILLA- GRANADA	Parque Tecnológico de Ciencias de la Salud. Avda. del Conocimiento s	Spain	Parasites, lipid translocation, Aminophospholipid translocases, ABC transporters.	Lipid translocation in arasites of health interest: their implications in the biology of parasites, and their potential use as drug targets. Aminophospholipid translocases and ABC transporters in parasites: its role in drug resistance and infectivity of parasites.	scientific
IQAC/Research Unit on BioActive Molecules	CSIC	34-93-4006115	08034	Barcelona	Jordi Girona 18	Spain	combinatorial chemistry, nuclear magnetic ressonance, drug discovery, environmental contaminants	Drug discovery and pharmacological tools: Effects of combinatorial libraries of synthetic chemicals on cell sphingolipid profiles. Toxicity of environmental contaminants: Deciphering whether the toxicity of selected environmental contaminants occurs by altering the sphingolipid composition of affected cells. Sphingolipid maps as sensors of environmental contamination. Analytical methodology: (1) Development of 15N and 31P NMR procedures to construct sphingolipid and phospholipid maps. (2) Application of biocompatible chemical reactions to the construction of pseudolipid maps and validation of this approach to research in cell biology.	scientific
Unidad de Biofísica	CSIC and Universidad del País Vasco	++34-94-601-2625	48940	Leioa	Sarriena s	Spain	membrane lipids, lipid biophysics, membrane domains, lipid-protein interaction	We are interested in the biophysics of membrane lipids, and lipid-protein interactions, with an emphasis on sphingolipids, lipid signalling, membrane domains, and, more recently, lipoproteins. Our techniques include calorimetry (DSC, ITC), spectroscopy (IR, fluorescence, UV-vis, stopped-flow, CD), confocal microscopy, Langmuir balance, and supported membranes.	scientific
National Center of Biotechnology	Spanish Research Council	++34-91-5854840	28049	Madrid	Darwin, 3. Campus Cantoblanco University	Spain	signaling, chemotaxis, leukocyte, HIV	The previous work of my group has been focused in understand how lipid rafts organize cell signaling during leukocyte chemotaxis as well as in the pivotal function of these microdomains at the early steps of the human immunodeficiency virus infection.	scientific
Institute of Molecular Biology and Genetics	Spanish Research Council & University of Valladolid School of Medicine	[phone]	E-47003	Valladolid	Calle Sanz y Fores s	Spain	Phospholipase A2, Arachidonic Acid, Prostaglandins, Lysophosphatidylcholine	Eicosanoids, Lysophospholipids, Glycerophospholipids	scientific
BIOMIL (BIOphysics of Membranes and Lipid/Protein Interfaces)	Universidad Complutense	++34-91-3944994	28040	Madrid	Fac. Biologia, Dept. Bioquimica	Spain	pulmonary surfactant, monolayers, membrane domains, surface activity	Membrane lateral structure and its role in the establishment of lipid-protein and protein-protein interactions. Lipids and lipid/protein complexes as surface-active agents, with particular emphasis in the structure and molecular mechanisms of membranes and surface layers of natural pulmonary surfactant and clinical preparations used in the therapeutical treatment of respiratory diseases.	scientific
Research Group in Biomembranes, Department of Biochemistry and Molecular Biology	University of Murcia	++34-968364766	E-30100	Murcia	Facultad de Veterinaria, Campus de Espinardo	Spain	lipid-protein interaction, PKCs, diacylglycerol, liposomes	Protein-lipid interactions: interactions with model membranes of signalling proteins, such as PKCs and Bcl-2 family of proteins associated to the regulation of cell apoptosis. Lipid regulation of the activity of PKCs. The action of a variety of bioactive lipìds on membrane translocation and activation of the Protein Kinase C family of proteins associated to cell signalling. The action of lipids such as phosphatidylserine, phosphoinositides, free fatty acids, diacylglycerols and ceramides is invetigated. Techniques used: NMR (CPMAS, HRMAS, deuterium NMR, 31P-NMR), DSC, ITC, Biacore, Fluorescence spectroscopy, confocal microscopy, X-ray diffraction.	scientific
Dept. Physiology, Medical School	University of the Basque Country	++34946012846	48940	Bilbao	Sarriena	Spain	Cholesterol, Liver, VLDL secretion, functional genomics	Disorders of lipid and lipoprotein metabolism involved in hepatic and cardiovascular diseases. Molecular mechanisms underlying nonalcoholic fatty liver disease. Functional genomics in nonalcoholic fatty liver disease.	scientific
The Wenner-Gren Institute	Stockholm University	++46-8-164127	SE10691	Stockholm	Arrhenius lab. F3	Sweden	fatty acid metabolism, elongase, gene expression	Our topic is the regulation of very long chain fatty acid syntersis in mammals and their significance in lipid metabolism.	scientific
Department of Crop Science	Swedish University of Agricultural Sciences	please fill in	23053	Alnarp	Box 44	Sweden	plant oil biosynthesis	please fill in	scientific
Department of Biophysical Chemistry	Umeå University	[phone]	SE-90187	Umeå	Linaeus väg 10	Sweden	domains, lateral diffusion, Lipid/protein	Lipid/protein interaction, regulation of lipid composition in cell membranes, Formation of domains in lipid membranes, Lipid lateral diffusion, Molecular ordering in membranes,.	scientific
LC-MS Support	Applera Europe B.V.	[phone]	6343	Rotkreuz	Grundstrasse 10	Switzerland	phospholipid, eiscosanoids, PAF, biomarker	Understanding of succession at developing and self stabilising cell populations and response/adaptation in the membrane lipid composition to stress conditions. Reveal cell signaling processes and interactions with the environment depending on lipid molecules. Lipid synthesis and degradation depend on enzyme activities and specific genes. Supressing specific lipid synthesis process or facilitating adaptation processes may identify genes involved and clarify function of so far unknown gene sequences. Assessment of regulatory lipids (eicosanoids, PAFs) as presymptomatic harbingers of pulmonary pathobiology. Development of rapid and easy-to-use methods for assessemnt and analysis of phospholipid profiles based on mass spectrometry. Rapid and sensitive quantification of signaling lipid molecules. Since 2007 LC-MS Support for Switzerland and South of germany Area, providing expertise in Lipidanalysis by LC-MS	industrial
Life Sciences Mass Spectrometry	School of Pharmaceutical Sciences - University of Geneva	[phone]	1211	Geneva	Bd Yvoy 20	Switzerland	Mass spectrometry, Pharmaceuticals, Proteins, C.elegans	please fill in	scientific
University of Basel, Experimental Immunology	University Hospital, Department of Research	[phone]	4031	Basel	Hebelstrasse, 20	Switzerland	immune recognition of lipids	Identification of llipids with immunogenic activity and involved in activating lipid-specific T cells in diseases	scientific
Division of Biochemistry	University of Fribourg	0041 26 300 8630	CH-1700	Fribourg	5, chemin du musée	Switzerland	Ceramide, GPI, cell wall, heat shock, cholesterol, ergosterol, steryl esters, lipases, lipid bodies	Biosynthesis, Remodeling and transport and cell wall integration of GPI anchored proteins of Saccharomaces cerevisiae. Sphingolipid biosynthesis of S. cerevisiae. Sterol transport, neutral lipid storage and degradation. Lipid rafts transport and sortining of integral membrane proteins.	scientific
Institute of Cellular and Organismic Biology, Section of Stem Cells	Academia Sinica	++886-2-27899531	11529	Taipei	Nankang	Taiwan	farnesyl pyrophosphate, geranylgeranyl pyrophosphate, E-Ras	Prenyl pyrophosphates as differentiation signals for stem cells	scientific
Institute of Bioinformatics and Structural Biology	National Tsing Hua University	886-3-5742752	30043	Hinchu	Kung-Fu Rd	Taiwan	glycosphingolipid, cardiotoxin, phospholipase A2	Our main interest is to understand the role of various lipids and/or lipid domain responsible for the action of cobra venom components such as cobra cardiotoxins or phospholipase A2.	scientific
Dept. of Medical Biochemistry & Immunology, School of Medicine	Cardiff University	0044 29 2074 8447	CF14 4XN	Cardiff	Heath Park	United Kingdom	please fill in	please fill in	scientific
Chemical Engineering Life Science Interface	Chemical and Process Engineering University of Sheffield	[phone]	S3 7RD	Sheffield	40 LeavyGreave Road	United Kingdom	Sulfolipids, sulfoglycolipids, cyanobacteria, bioactive compounds	My area of interest is to mining lipid profile of cyanobacteria for identifying potential drug candidates.	scientific
School of Life Sciences	Heriot-Watt University	++44 131 451 3186	EH14 4AS	Edinburgh	Roccarton	United Kingdom	Diet-gene interaction, lipogenesis,fungal lipid metabolism	We are interested in the influence of diet on the regulation of gene expression in hepatic tissue culture, in particular emphasis on lipogenic genes. A second area of interest focusses on fungal lipid metabolism, particularly in the oleaginous Yarrowia lipolytica.	scientific
The Chemical Biology Centre in the Department of Chemistry	Imperial College London in association with the Institute of Cancer Research and the London Research Institute of CRUK	[phone]	SW7 2AZ	London	Exhibition Road	United Kingdom	Lipid biophysical characterisation	Biophysical properties of lipids Phase behaviour studied by SAXRD, NMR, SSNMR, polarising microscopy, DSC, high pressure SAXRD Mesophase structure and energetics measured by SAXRD osmometry, SSNMR, ITC, DSC, direct vesicle manipulation and modelling using continuum elastic theories, mesoscopic modelling and full atomistic modelling (QMMD) Dynamics of phase transformations (the lipids role in cell division, fusion etc.) using synchrotron X-ray scattering on rapidly perturbed systems Studies of the short and long range coupling of membrane charge and curvature elasticity Lipid-protein and lipid-drug interactions Studies of the effect of membrane elasticity on enzyme activity, extrinsic membrane protein binding, membrane protein refolding dynamics, drug binding Studies of the coupling of membrane chemistry and biochemistry to lipid elastic properties Studies of lipid liquid ordered phases and protein associations within these domains Studies of membrane protein assembly, distribution and dynamics Development of novel single cell membrane proteomics and lipidomics A recently funded project to handle single cells inoptical traps within microfluidic flows, strip off membrane portions, separate components and analyse using a novel 2D-IR spectroscopy	scientific
Nature Reviews Molecular Cell Biology	NPG	[phone]	N1 9XW	London	4 Crinan St	United Kingdom	lipids	lipids	scientific
Glycobiology Institute	Oxford University	[phone]	OX1 3QU	Oxford	South Parks Rd	United Kingdom	Oligosaccharide analysis, lysosomal storage	Protein and lipid glycosylation and diseases, including the lysosomal storage disorders (Gaucher disease for example). Glycolipid (sphingolipid) analysis by HPLC and mass spectrometry. Chemistry and biology of imino sugars that modulate glycolipid biosynthesis	scientific
Shell Global Solutions (UK)	Shell Global Solutions (UK)	[phone]	CH13SH	Chester	P.O. Box 1	United Kingdom	extraction analysis	Interest in extraction and analysis techniques for lipids	industrial
Refsum disease group	St Thomas Hospital	++442071881256	SE1 7EH	London	Lambeth Palace Road	United Kingdom	peroxisome, alpha-oxidation, omega-oxidation, isoprenoid, phytanic	The group has been interested in the transport and biochemical pathways of phytanic acid metabolism and thus alpha- and omega-oxidation pathways of alpha-methyl isoprenoid fatty acids. This pathway has recently been defined with the cloning of key enzymes in alpha-oxidation including phytanoyl-CoA 2-hydroxylase (PAHX), 2-hydroxyphytanoyl-CoA lyase, alpha-methylacyl-CoA racemase together with confirmation of their localisation in peroxisomes. PAHX, an iron(II) and 2-oxoglutarate dependent oxygenase is located on chromosome 10p13. Mutant forms of PAHX have been shown to be responsible for some, but not all, cases of Refsums Disease. Certain cases have been shown to be atypical mild variants of rhizomelic chondrodysplasia punctata type 1a. Other atypical cases with low plasma phytanic acid may be caused by a-methylacyl-CoA racemase deficiency. A sterol-carrier protein 2 (SCP-2) knockout mouse model shares a similar clinical phenotype to Refsums Disease, but no mutations in SCP-2 have been described to date in man. SCP-2 acts as a solubilistaion factor/intracellular carrier for these hydrophobic fatty acids. Work on the omega-oxidation pathway for these fatty acids has clarified the role of cytochrome 4A1 enzymes in the intitial hydroxylation to dicarboxylic acids and the subsequent peroxisomal beta-oxidation pathway. It has also led to interest in this pathway as a therapeutic option for peroxiosomal diseases. The pathway for synthesis of phytanic acid from phytol in man and possibly other mammals has also been clarified with the identification of the enzymes in the pathway and identification of FALDH-10 - the enzyme deficient in Sjogren-Larsson syndroem as one of the key enzymes in this pathway. The group is also interested in the role of PhyH outside the preroxisome where it may act as protein regulator and the possible importance of phytanic acid as a PPAR-alpha ligand.	scientific
Mass Spectrometry Facility	The School of Pharmacy	++44-20-77535876	WC1N 1AX	London	29/39 Brunswick Square	United Kingdom	sterols, steroids, lipid rafts	The research in the mass spectrometry group at the School of Pharmacy is focused on lipids and their interactions with proteins. Major efforts are being made in the development of new methodology for the mass spectrometric analysis of steroids and sterols. In parrallel with lipidomic studies, we are investigating the protein content of lipid rafts and studying protein-lipid interactions.	scientific
European application laboratory	Thermo Electron	[phone]	HP2 7GE	Hemel Hempstead	1 Boundary Park	United Kingdom	mass spectrometry, phospholipids, sphingolipids	methods in mass spectrometry for high throughput lipid analysis	industrial
WELLCOME TRUST BIOCENTRE, SCHOOL OF LIFE SCIENCES, UNIV OF DUNDEE	UNIV OF DUNDEE	++44-(0)1382-388688	DD1 5EH	DUNDEE	HAWKHILL	United Kingdom	PROTOZA LIPID BIOSYNTHESIS	MY GROUP WORKS ON PHOSPHO- AND GLYCO- LIPID BIOSYNTHESIS IN PROTOZOAN PARASITES SUCH AS T.BRUCEI, THE CAUSATIVE AGENT OF AFRICAN SLEEPING SICKNESS. WE USE TOOLS SUCH AS FOWARD AND REVESRE GENETICS TO VALIDATE GENES AS DRUG TARGETS PRIOR TO EXPLORING SUBSTRATE AND INHIBITOR SELECTIVITY. WE HAVE START TO SET UP A PARASITE LIPIIDOME DATABASE FOR THE COMMUNITY TO USE AND SUBMIT PROFILES TO.	scientific
Cardiovascular Genetics,	University College London	[phone]	WC1E 6JF	London	5 University St	United Kingdom	genes, apolipoproteins, triglyceridie metabolism	Cardiovascular Genetics	scientific
Aberdeen lipidomix group	University of Aberdeen	44 (0)1224 553020	AB25 2ZD	Aberdeen	Polwarth building, Foresterhill	United Kingdom	inflammation-atherosclerosis-endothelial fonction-diet	function of biologically active lipids in cardiovascular disorders. Effect of dietary components on the expression and secretion of biologically active lipid derivatives in relation to inflammatory disorders.	scientific
Department of Pharmacy & Pharmacology	University of Bath	++-44-1225-3867686	BA2 7AY	Bath	Claverton Down	United Kingdom	Enzyme, phytanic acid, isoprenoids, long-chain fatty alcohols	Enzaymes involved in lipid metabolism, branched-chain lipids and their role in diseases, long-chain fatty alcohols,	scientific
Institute for Cancer Studies	University of Birmingham	44-(0)121-414-3293	B15 2TT	Birmingham	Vincent Drive	United Kingdom	phosphoinositide, mass spectrometry, phospholipase, cancer	1. Analysis of lipid signalling in particular phospholipases D and C and PI-3-kinase. 2. Use of MS methods to analyse lipids in mammalian, dictyostelium, yeast and drosophila cells in order to determine functions of signalling pathways. 3. Analysis of lipids in tumour cells isolated by techniques such as laser capture microdissection. 4. Analysis of changes in lipids in other diseases for example vasculitis. 5. Development of MS methods to quantify all phosphoinositides.	scientific
School of Pharmacy	University of Bradford	++44-1274-224717	BD7 1DP	Bradford	Richmond Road	United Kingdom	eicosanoids, brain, skin, mass spectrometry	eicosanoids and other lipid mediators molecular mechanism of action of omega-3 fatty acids with emphasis on eicosapentaenoic acid eicosanoid-mediated pathways and signalling systems mass spectrometry high field NMR computational lipidomics cardiovascular disease neurodegenerative diseases brain function cancer melanocytes	scientific
Department of Pharmacology	University of Cambridge	44-1223-334032	CB2 1PD	Cambridge	Tennis Court Road	United Kingdom	multidrug transporters, steroids, structure/function	We study the molecular bases of the interactions of ATP-binding cassette transporters of human (e.g. ABCG1 and ABCG2) and bacterial origin (e.g., MsbA and LmrA) with chemotherapeutic drug and steroids and other lipids. We also study the potential physiological roles of multidrug transporters in lipid transport.	scientific
Dept. of Biochemistry	University of Oxford	[phone]	OX1 3QU	Oxford	South Parks Road	United Kingdom	membrane protein	membrane protein/lipid interactions biomolecular simulations	scientific
School of Chemistry	University of Southampton	++44 (0)2380 796161	SO17 1BJ	Southampton	University Road	United Kingdom	phospholipids, mass spectrometry, dynamic lipidomics	Mass spectrometry of lipids Dynamics of phospholipid synthesis Modelling lipid synthetic networks Lipid biomarkers in health and disease Synthesis, composition and function of endonuclear lipids Lung surfactant in health and disease Interaction between genotypic expression and diet in the regulation of the molecular species composition of cell lipids in vivo and in vitro Phospholipase-mediated cell signalling Membrane fusion Lipids in stem cell differentiation Lipids and nutrition Lipids in inflammation Oxidised phospholipids and cardiovascular disease	scientific
Centre for Equine and Animal Science	Writtle College	44 1245 424200	CM1 3RR	Chelmsford	Lordship Road	United Kingdom	poyunsaturated fatty acids, conjugated linoeic acids, immunity, health	Nutritional and biochemical role of fatty acids in the health and performance of farm and companion animals.	scientific
Centre for Equine and Animal Science	Writtle College	44 1245 424200	CM1 3RR	Chelmsford	Lordship Road	United Kingdom	poyunsaturated fatty acids, conjugated linoeic acids, immunity, health	Nutritional and biochemical role of fatty acids in the health and performance of farm and companion animals.	scientific
University of Iowa	Dept. of Molecular Physiology and Biophysics	1-319-335-7874	52246	Iowa City	6-530 Bowen Science Building	United States	multidrug resistance, sphingolipids, mitochondria	We study the interaction of sphingolipids and phospholipids with multidrug transporters in the yeast Saccharomyces cerevisiae. Transcription factors that modulate the expression of membrane transporters, often in the plasma membrane, have recently been found to also control the expression of genes involved in sphingolipid biosynthesis. Our goal is to understand the physiological rationale this coordinate control.	scientific
Indiana Umiversity -Purdue University Indianapolis	Indiana Umiversity -Purdue University Indianapolis	317-274-0593	46202	Indianapolis	723 W. Michigan St.	United States	yeast, sterol, ergosterol	yeast sterol biosynthesis	scientific
Institute of Molecular Medicine and Genetics	Medical College of Georgia	01-706-721-0699	GA 30912	Augusta	1120 15th Street	United States	Sphiogolipids, glycolipids, developmental biology, neurodegenerative diseases, neurochemistry	Analysis of glycolipid structure and metabolism in the nervous system, lipid signaling molecules and signal trnaduction, cell-cell recognition and adhesion, cell migration.	scientific
COBRE in Lipidomics & Pathobiology	Medical University of South Carolina	843-792-4323	29425	Charleston	PO Box 250509	United States	cell growth, cell death, cell aging, inflammation	Define the function of these fatty molecules in human disease, especially cancer, aging, neurologic disease and fungal pathogenesis.	scientific
COBRE in Lipidomics & Pathobiology	Medical University of South Carolina	843-792-4323	29425	Charleston	PO Box 250509	United States	cell growth, cell death, cell aging, inflammation	Define the function of these fatty molecules in human disease, especially cancer, aging, neurologic disease and fungal pathogenesis.	scientific
Center for Developmental Genetics	Stony Brook University	please fill in	11794	Stony Brook	438 CMM	United States	please fill in	please fill in	scientific
The Polt Group	The University of Arizona	++01-520-621-6322	AZ 85721	Tucson	Department of Chemistry	United States	PDMP, glycosphingolipid, sphingosine	Glycosphingolipids. Synthesis and structure elucidation. We are involved with the design and synthesis of glycosidase and glycosyltransferase inhibitors. We use Manduca sexta (tobacco horn worm) as a model system to explore the effects of GSLs on development. http://www.chem.arizona.edu/faculty/profile/profile.php?fid_call=polt	scientific
San Diego Supercomputer Center / LIPID MAPS Bioinformatics Core	University of California San Diego	858-822-3619	92093	La Jolla	9500 Gilman Drive, Mail Code 0412	United States	lipid classification, lipid database, lipid proteome, lipid pathways	The LIPID Metabolites and Pathways Strategy (LIPID MAPS) Consortium represents a multi-institutional effort to develop a detailed understanding of lipid structure and function. As part of this effort, we will develop ‘parts lists’ of lipid metabolites and assemble these into metabolic networks. These networks will then provide an infrastructure for subsequent modeling using quantitative data from LIPID MAPS experiments.	scientific
Nutrition and Genomics	USDA-Human Nutrition Research Center on Aging at Tufts University	++1-617-556-3102	02111	Boston	711 Washington St	United States	genetic polymorphisms, gene-diet interactions, perilipins,	The Nutrition and Genomics Laboratory has been pionnering the study of gene-diet interactions in the area of cardiovascular diseases, utilizing both genetic epidemiology approaches as well as controlled dietary intervention studies. This research involves the investigation of nutrient-gene interactions in large and diverse populations around the world with long-standing collaborations with investigators in Europe, Asia, Australia and the United States. More recently our interest has been focusing on the genetic factors involved in fat metabolism in the adypocite and more specifically the PATS family of genes. In addition, we seek to identify genes involved in longevity and healthy aging and to understand their regulation in response to dietary factors. For this research we use model organisms such as Drosophila and mouse. Further comprehension of the relation between genetic factors, nutrients and the rate of aging will provide better understanding of the pathology of age-related diseases and lead to improved strategies for their prevention.	scientific
Department of Biochemistry	Wake Forest University School of Medicine	please fill in	27157	Winston-Salem	Medicial Center Boulevard	United States	phospholipase D, bicelles, eicosanoids, lipoproteins, lipid kinases, virus assesmbly	The Department of Biochemistry (and affiliated faculty) at Wake Forest University has a working group of faculty members (about 14 faculty members) interested in the role of lipids in signal transduction, atherosclerosis, cancer, host defense, and inflammation. Techniques in use include thin-layer chromatography, HPLC, and mass spectrometry to identify phospholipid and fatty acid species, vesicle and solid-phase lipid binding assays, NMR spectroscopy and other biophysical approaches, coupled with site-directed mutagenesis, to study structural features of lipid:protein interactions, and computational modeling of signaling pathways involving lipids. Professor McPhail represents the lipid signaling working group on the departmental Development Committee, which advises the Chair on departmental development and policy.	scientific