Designation : Assistant Professor Address
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- Rama S Verma, Bijesh K. Biswal. Abhilasha, Chithra R and Sugapriya D. M. (2009) Innovation and Challenges in Biotechnology (ICICB-08) ISBN: 978-93-80043-16-6
- RESEARCH INTEREST Project 1
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Dr. Sugapriya. D Mobile No: 00966-532094216 E-mail: sughaphd@gmail.com 1. Designation : Assistant Professor 2. Address : Department of Medical Laboratory Sciences (Haematology/Microbiology), College of Applied Medical Sciences, Salmon Bin Abdulaziz University, Wadi Ad Dawasir, Riyadh, Kingdom of Saudi Arabia. 3. Phone Number’s : 00966-532094216 4. E-mail : sughaphd@gmail.com 5. Date of Birth : 03.09.1977 5. Education (Highest Degree): Degree : Ph. D. (2008) University : University of Madras, India Specialization : Cancer Biology Thesis Title : Biochemical, cellular and molecular studies on the pharmacological effects of Semecarpus anacardium Linn on Bcr-Abl+ experimental leukemic model with special reference to apoptosis. Education Qualification:Apr 2004 - Aug 2008 Doctorate of Philosophy (Ph.D.) in Microbiology with Pathology from University of Madras, TN, India. Jun 2001- Aug 2002 Master of Philosophy (M.Phil) in Microbiology from PRC affiliated to Bharathidasan University, TN, India, Percentage of marks 75.9 and year of passing 2002. Jun 1998- Apr 2000 Master of Microbiology (M.Sc) from V.H.N.S.N College affiliated to Madurai Kamarajar University, TN, India, Percentage of marks 68.9 and year of passing 2000. Jun 1995- Apr 1998 Bachelor of Microbiology (B.Sc) from Muthayammal College affiliated to University of Madras, TN, India, Percentage of marks 68.9 and year of passing 1998. Career Summary:
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ACADEMIC RESEARCH AND TEACHING APPOINTMENTS:
* This work is awarded for travel grant of USD$ 1250 and registration fee (USD$ 600) in 10th International Stem cell Society for Research (ISSCR) at Yokohama, Japan. INTERNATIONAL REFEREED JOURNALS (STARTING FROM RECENT)
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RESEARCH INTERESTProject # 1IIT Madras, Chennai, India Feb 2010 – Feb 2013 Project: Induction of Functional Hepatocyte-Like Cells from Mouse Mesenchymal Stem Cells by FOXA-2 for Liver Development Short Abstract: Induced hepatocyte-like cells have multiple hepatocyte-specific features and aids in reconstituting damaged hepatic tissues after transplantation. To differentiate mouse mesenchymal stem cells into functional hepatic cells using FOXA-2 a master regulator of liver-specific gene expression for liver development. In this study, we examined the role of FOXA-2 in hepatic differentiation from mouse MSCs, which were efficiently generated by transfection and then the expression of hepatic markers of the hepatocyte-like cells were assessed. Outcome: We have developed a robust and efficient method to differentiate mesenchymal stem cells into hepatic like cells, which exhibits characteristics of mouse hepatocytes. Our approach would facilitate the development of hepatocytes for liver engineering and regenerative medicine. Responsibilities:
Experimental platform: Stem Cell, Cloning of gene interest, Immunocytochemistry, Multilineage differentiation, RNA isolation, Real-Time PCR, Western Blot, PAS (Glycogen storage), Biochemical analysis. Project # 2IIT Madras, Chennai, India Feb 2010 – Feb 2013 Project : Development of a Simplified Protocol for Isolation and Characterization of Liver-Derived Stem Cell (LDSCs) from Neonatal Mice Short Abstract: The ability to isolate and expand liver-derived stem cells (LdSCs) is an important step in the development of tissue engineering approaches for liver repair or regeneration for the treatment of liver damage, as well as for their application in plastic or reconstructive surgery. Here, we describe step-by-step procedures on the basis of frequent medium change in primary culture and diminishing the trypsinization time. In this protocol, we have shortened the enzyme digestion period and LdSCs enrichment step. Outcome: The present study describes an improved method for rapid isolation of LDSCs from neonatal mice, as well as the maintenance and propagation of such cultures for the long term. After isolation and culture, these cells behave similarly to those in vivo, including their ability to proliferate, providing an ideal system for the study of hepatic stem cell proliferation and multilineage differentiation. The successful isolation and cultivation of LdSCs will allow to study their unique biological properties which have almost unlimited proliferation capabilities while retaining the potential to differentiate in vitro into various progenitor used for therapeutic approaches. Responsibilities:
Experimental platform: Liver derived stem cell, Immunophenotyping, Immunocytochemistry, Multilineag differentiation, RNA isolation, RT- PCR. Project # 3IIT Madras, Chennai, India Aug 2008 – Jan 2009 Project: Augmented Sensitivity to Methotrexate by Curcumin induced overexpression of Folate Receptor in KG-1 Cells Short Abstract: Folate receptors are targets of various strategies aimed at efficient delivery of anti-cancer drugs. Therefore, it is important to identify agents which increase expression of folate receptors in cancer cells. The present study aimed to investigate the role of curcumin in augmenting expression of folate receptors in leukemic tumor cells. Outcome: Our results strongly suggest that curcumin’s ability to enhance the cytotoxicity of methotreate in KG-1 leukemic cells, occurs via up-regulation of expression of folate receptor β protein and enhancement of methotreate transport. We hypothesize that administering optimal concentration of curcumin in combination with low-dose of methotreate, could be a promising strategy for treatment of leukemia. This strategy would potentially reduce the genotoxic effects associated with methotreate treatment Responsibilities:
Experimental platform: Cancer cell line, Immunocytochemistry, Real-Time PCR, Western blot, Transport mechanism by liquid santillation Counter. Project # 4University of Madras, Chennai, India April 2004 – April 2008 Ph.D. Thesis Topic: Biochemical, cellular and molecular studies on the pharmacological effects of Semecarpus anacardium Linn on Bcr-Abl+ experimental leukemic model with special reference to apoptosis. Summary of the Thesis: Chronic Myeloid Leukemia (CML) is a malignant clonal disorder of haematopoietic stem cells leading to massive expansion of myeloid lineage cells at all stages of maturation and development. CML is associated with a specific chromosomal translocation known as the “Philadelphia (Ph) chromosome” t (9; 22) (q34; q11), which is a BCR-ABL translocation; this translocation encodes for the BCR-ABL fusion protein, which is a constitutively active tyrosine kinase. A Tyrosine kinase inhibitor, Imatinib (Gleevac) which specifically inhibits BCR-ABL is being used in the chronic phase and in blast crisis. However, imatinib also has side effects. Moreover, the innumerable reports in literature of patients developing resistance to imatinib are of great concern. Thus the search for newer, more effective antileukemic drugs with fewer side effects continues. Semecarpus anacardium Linn.nut milk extract (SA) has already been shown to have anticancer activity in experimental breast cancer and hepatocellular carcinoma. Using the 12B1 BCR-ABL+ murine cell line (in vitro) and a murine model (in vivo) of BCR-ABL+ leukemia, we have evaluated SA for its antileukemic potential, its possible mode of action via the apoptotic pathway and restorative efficacy on the biochemical and apoptotic changes observed in the disease condition. The study included assessment of altered marker and lysosomal enzymes, antioxidant and non antioxidant levels at serum, liver and spleen level, altered in energy metabolism, apoptotic bodies (TEM) study, macromolecular damages like single strand DNA breaks and DNA-protein cross links (in vivo). Drug induced apoptosis was studied in vitro by using 12B1 leukemic cell line. Propidium iodide, DNA fragmentation, and protein and mRNA expression of Bcl-2, Bax, Cytochrome c, p53, caspase-3 and 9 by western blot and RT- PCR and the mRNA expression of BCR-ABL was studied by RT-PCR to confirm that the SA induced apoptosis through mitochondrial mediated pathway and also inhibit the BCR-ABL mRNA. Our study concludes the chemotherapeutic action of SA and suggested it as a major therapeutic value against leukemia. Responsibilities:
Experimental platform: Induction of leukemia in experimental animal, Biochemical enzymatic analysis, TEM analysis, Histopathology, RT-PCR, apoptosis analysis, Western blot. Project # 5PR College, Bharathidasan University CLRI, Chennai Jan 2002 – Aug 2002 M.Phil Project: Perturbation and Dysfunction of Intracellular Ca2+ Homeostasis in Mitochondria by Novel Amyloid Forming Model Peptide Poly (Leucine-Glutamic Acid) Short Abstract: Amyloid forming model peptide Poly (Leucine-Glutamic acid) (LE) toxicity on peripheral blood mononuclear Cells (PBMC) are mediated by oxidative stress inducing apoptosis and macromolecular damage with depletion of GSH, leads to decreases PBMC cell viability. Cell death was prevented by the antioxidant vitamin E shows to be effective in reducing the accumulation of reactive oxygen species (ROS) in cells exposed to poly (LE). Enhanced ROS increases oxidized protein concentration and the leakage of Ca2+ ions from the mitochondria and finally leads to significant increase of intracellular calcium. Based on the above findings, we postulate that poly (LE) fibril directly involves in the disruption of mitochondrial function, contribute to the deficiency of energy metabolism and increased calcium homeostasis leads to DNA fragmentation. Outcome: In conclusion, our studies demonstrate that oxidative stress of poly (LE) fibril toxicity leads to mitochondrial dysfunction responsible for spontaneous generation of intracellular ROS. Present study suggests that vitamin E could be used for prevents the formation of amyloid plaques and we hypothesized that vitamin E may also have some role in reducing the fibril formation of poly (LE) in neurodegenerative diseases such as alzheimer’s and in self-assembly of peptides. Responsibilities:
Experimental platform: MTT assay, Measurement of intracellular ROS, Analysis of mitochondrial membrane potential, Intracellular calcium levels, Quantification of endogenous GSH, DNA fragmentation. Project # 6V.H.N.S.N. College, Madurai Kamarajar University, Nov 1999 – April 2000 M.Sc Project: Production of Streptomycin and compare the individual and combined effect with penicillin for various organisms. Short Abstract: Production and purification of antibiotic from rhizospere isolates and compare the individual and combined effect for various organisms. Isolation of various organisms from rhizospere region for production of streptomycin and penicillin antibiotic. Selection and characterization of strains produces more antibiotic from the isolates. Production and purification of these antibiotics by column chromatography. Compare these antibiotic effects by individual and combination with various organisms. Outcome: In our study the yield of Streptomycin and penicillin production from Rhizosphere isolates is equal to that of ATTC culture. When compare with the individual effects the combined effect is highly sensitive to the penicillin resistant organisms. Responsibilities:
Experimental platform: Column Chromatography, Antibiotic sensitivity assay. Project # 7Muthayammal College, University of Madras, Nov 1997 – Mar 1998 B.Sc Project: Effect of antimicrobial activity on Coleus aromaticus against gram negative organisms. Short Abstract: Study the effect antimicrobial activity of Coleus aromaticus against gram negative enterobacteriaceae. Collection of Coleus aromaticus leaf and stem dried separately in shades. Prepare water extract and methanol extract separately used for further analysis. Enterobacteriaceae organisms were used for the study of antimicrobial assay for methanol and water extract. Outcome: In our study the leaf extract is highly effective against the Enterobacteriaceae when compare with stem extract. Methanol extract is less effective than water extract. Therapeutic efficacy of Coleus aromaticus is effective to Shigella when compare all other Enterobacteriaceae. Responsibilities:
Experimental platform: Extract preparation, Antibiotic sensitivity assay. Technical / Functional (Application) Skills
Leadership Experience Over the past eight years I had the blessing to be a small youth group leader to junior project students during my Ph.D. (4 Nos) and in my Postdoctoral work (6 Nos). I meet face to face with them daily for 6 month and most of the time I just talk with them about their research interest, explaining about the research work, problem of their work, solving the problem of contamination in cell culture. During my career as lecturer I was as a mentor for 2 M.Sc students for their research project. I proceeded to tell discuss them about their work and future work plan. I once had that ended badly but affirmed how they dealt with correctly and how they should go about finishing their work strong. During my postdoctoral work I was an organizer committee member for Ist International stem cell Submit 2008 held in IIT-Madras, India. For my postdoctoral work I wrote a project proposal and proposal was sanctioned by Department of Science and Technology, Government of India. Workshop Training “Animal Cell Culture” on IIT Madras QIP Short Term Training Programme 2008-09 on 20-24th July, 2009, held at Indian Institute of Technology Madras, Chennai. Indo-UK Workshop on Designing of Doctoral Research for Ph.D Scholars held on 23 rd March 2005 at Chennai, Tamil nadu, India. International Seminor on Immunotechnology and workshop on immunoenzyme labeling in tissue sections, ELISA, 2D & Pulse Field Gel Electrophoresis and Fermentor held from 20th - 22nd October 2005 at Chennai, Tamil nadu, India. IIIrd SURGICAL PATHOLOGY UPDATE held on 1st February 2008 at Chennai, Tamil nadu, India. Certificate in Bioinformatics – Anna University, MIT Campus, Chennai, India-2005. Summary of Teaching Experience and Pedagogy Brief employment history I have taught for a little over a decade as a Lecturer in private college affiliated to University of Madras.. During this time I have taught at three different Colleges, the Bharathi College of arts and Science, Valliammal College, KRMMC College. While my appointments at KRMMC have been in their Biotechnology Departments and other institutions were as in Microbiology Department. I have also taught in programs related to my research such as Molecular Biology, Animal biotechnology, General microbiology, practical for molecular biology and practical for animal biotechnology in KRMMC College; In Valliammal College I have taught Medical microbiology, Bacteriology and practical for Bacteriology and in Bharathi college I have taught Medical microbiology, Molecular biology, Immunology and practical for molecular biology and immunology. I design my courses with three goals: 1) introduce students to major scholars and themes in the field as it relates to the specific course topic; 2) further develop students’ confidence as intellectuals and thinkers; and 3) further develop students’ practical skills and increase their employability. Of course, proper assessment of these and all other activities must be undertaken to ensure that the desired educational goals are being met. In designing assessment tools (notably exams, term papers and lab reports), I again attempt to challenge students to reach beyond the mere facts and to demonstrate critical thinking and a deeper understanding of the subject matter. For this purpose, I utilize predominantly essay and long answer exams, especially in the upper level and graduate courses. After the exam, I provide the answers (via Moodle) before handing back the exams (or before even posting the grades) in the hopes that students will review the exam as an added reinforcement. More recently, I have invited students to use the posted answers to remember and “grade” their exam answers in advance of receiving the actual graded exam, including of details of how and where points would be taken off. With the enticement of extra credit for this assignment, most of the class took part and I believe that this was an even better reinforcement of the material. Additionally, for all written work handed in, I try to provide constructive criticism so that students are not merely earning a grade on these assignments, but can learn how to create an even better product and gaining from the assessment process. My experiences to date suggest that at least at some level, I am succeeding in my undertakings as teacher. However, I am well aware that there is my room for me to evolve and grow and that I can also actively learn to be a better teacher - from my colleagues and from students’ learning experiences. I believe that my goal of becoming an outstanding teacher is a journey rather than a destination. I am excited to continue on this journey. I declare that all information provided with this application is true to the best of my knowledge and belief,
Signature of Applicant Sugapriya Dhanasekaran Yüklə 81,5 Kb. Dostları ilə paylaş:
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